Literature DB >> 3031590

Nucleotide sequence of the melA gene, coding for alpha-galactosidase in Escherichia coli K-12.

P L Liljeström, P Liljeström.   

Abstract

Melibiose uptake and hydrolysis in E.coli is performed by the MelB and MelA proteins, respectively. We report the cloning and sequencing of the melA gene. The nucleotide sequence data showed that melA codes for a 450 amino acid long protein with a molecular weight of 50.6 kd. The sequence data also supported the assumption that the mel locus forms an operon with melA in proximal position. A comparison of MelA with alpha-galactosidase proteins from yeast and human origin showed that these proteins have only limited homology, the yeast and human proteins being more related. However, regions common to all three proteins were found indicating sequences that might comprise the active site of alpha-galactosidase.

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Year:  1987        PMID： 3031590      PMCID： PMC340628          DOI： 10.1093/nar/15.5.2213

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  29 in total

1.  A SECOND PERMEASE FOR METHYL-THIO-BETA-D-GALACTOSIDE IN ESCHERICHIA COLI.

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Journal:  Biochim Biophys Acta       Date:  1965-05-04

Review 2.  Prediction of the secondary structure of proteins from their amino acid sequence.

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Authors:  T Ikemura
Journal:  J Mol Biol       Date:  1981-02-15       Impact factor: 5.469

4.  A simple method for displaying the hydropathic character of a protein.

Authors:  J Kyte; R F Doolittle
Journal:  J Mol Biol       Date:  1982-05-05       Impact factor: 5.469

5.  Analysis of melibiose mutants deficient in alpha-galactosidase and thiomethylgalactoside permease II in Escherichia coli K-12.

Authors:  R Schmitt
Journal:  J Bacteriol       Date:  1968-08       Impact factor: 3.490

6.  Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: a proposal for a synonymous codon choice that is optimal for the E. coli translational system.

Authors:  T Ikemura
Journal:  J Mol Biol       Date:  1981-09-25       Impact factor: 5.469

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Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

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Authors:  H Grosjean; W Fiers
Journal:  Gene       Date:  1982-06       Impact factor: 3.688

9.  The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.

Authors:  J Shine; L Dalgarno
Journal:  Proc Natl Acad Sci U S A       Date:  1974-04       Impact factor: 11.205

10.  Role of inducer exclusion in preferential utilization of glucose over melibiose in diauxic growth of Escherichia coli.

Authors:  T Okada; K Ueyama; S Niiya; H Kanazawa; M Futai; T Tsuchiya
Journal:  J Bacteriol       Date:  1981-06       Impact factor: 3.490
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  20 in total

1.  Purification and characterization of the recombinant Thermus sp. strain T2 alpha-galactosidase expressed in Escherichia coli.

Authors:  M Ishiguro; S Kaneko; A Kuno; Y Koyama; S Yoshida; G G Park; Y Sakakibara; I Kusakabe; H Kobayashi
Journal:  Appl Environ Microbiol       Date:  2001-04       Impact factor: 4.792

2.  Overexpression, purification and characterization of the Escherichia coli MelR transcription activator protein.

Authors:  R Caswell; J Williams; A Lyddiatt; S Busby
Journal:  Biochem J       Date:  1992-10-15       Impact factor: 3.857

3.  Cloning and sequencing of the melB gene encoding the melibiose permease of Salmonella typhimurium LT2.

Authors:  K Mizushima; S Awakihara; M Kuroda; T Ishikawa; M Tsuda; T Tsuchiya
Journal:  Mol Gen Genet       Date:  1992-07

4.  Cosmid-derived map of E. coli strain BHB2600 in comparison to the map of strain W3110.

Authors:  R P Birkenbihl; W Vielmetter
Journal:  Nucleic Acids Res       Date:  1989-07-11       Impact factor: 16.971

5.  6-phospho-alpha-D-glucosidase from Fusobacterium mortiferum: cloning, expression, and assignment to family 4 of the glycosylhydrolases.

Authors:  C L Bouma; J Reizer; A Reizer; S A Robrish; J Thompson
Journal:  J Bacteriol       Date:  1997-07       Impact factor: 3.490

Review 6.  Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Authors:  M K Berlyn
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

Review 7.  Linkage map of Escherichia coli K-12, edition 8.

Authors:  B J Bachmann
Journal:  Microbiol Rev       Date:  1990-06

8.  Nucleotide sequences and operon structure of plasmid-borne genes mediating uptake and utilization of raffinose in Escherichia coli.

Authors:  C Aslanidis; K Schmid; R Schmitt
Journal:  J Bacteriol       Date:  1989-12       Impact factor: 3.490

9.  alpha-Galactoside uptake in Rhizobium meliloti: isolation and characterization of agpA, a gene encoding a periplasmic binding protein required for melibiose and raffinose utilization.

Authors:  D J Gage; S R Long
Journal:  J Bacteriol       Date:  1998-11       Impact factor: 3.490

10.  Cloning and high-level expression of alpha-galactosidase cDNA from Penicillium purpurogenum.

Authors:  H Shibuya; H Nagasaki; S Kaneko; S Yoshida; G G Park; I Kusakabe; H Kobayashi
Journal:  Appl Environ Microbiol       Date:  1998-11       Impact factor: 4.792
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