OBJECTIVE: Autologous chondrocyte implantation is a necessary procedure for the repair of articular cartilage defects; however, isolated chondrocyte implantation requires a 2-step procedure (for harvesting and implantation) and is limited by cytotoxicity due to enzymatic digestion. Therefore, in this in vitro study, we evaluated the possible benefit of using minced cartilage embedded in a 3-dimensional culture scaffold and fixed with fibrin glue, in comparison with isolated chondrocytes in atelocollagen, to induce cell migration, proliferation, and matrix production, using cartilage from patients with knee joint osteoarthritis. DESIGN: Cartilage fragments were obtained from 7 female patients with knee osteoarthritis (OA) and embedded in atelocollagen gels. As a control, chondrocytes were isolated and embedded in gels in the same manner. These composites were cultured for 3 weeks, and cell proliferation and matrix production were evaluated using histology and immunochemistry. RESULTS: Histologically, minced cartilage showed cell migration from the cartilage fragments into the gel, with the Bern score and cell count in the minced cartilage group being significantly higher than those in the control group. Immunohistochemistry revealed that the number of Ki67-positive cells, the expression of LECT-1 and TGF-β, and the glycosaminoglycan content were significantly higher in the minced cartilage than in the control group. Minced cartilage exhibited superior cell migration, proliferation, and glycosaminoglycan content than isolated chondrocytes. CONCLUSION: Our findings support that minced cartilage has a favorable potential for cell proliferation and matrix production compared with the isolated chondrocytes after enzymatic treatment.
OBJECTIVE: Autologous chondrocyte implantation is a necessary procedure for the repair of articular cartilage defects; however, isolated chondrocyte implantation requires a 2-step procedure (for harvesting and implantation) and is limited by cytotoxicity due to enzymatic digestion. Therefore, in this in vitro study, we evaluated the possible benefit of using minced cartilage embedded in a 3-dimensional culture scaffold and fixed with fibrin glue, in comparison with isolated chondrocytes in atelocollagen, to induce cell migration, proliferation, and matrix production, using cartilage from patients with knee joint osteoarthritis. DESIGN:Cartilage fragments were obtained from 7 female patients with knee osteoarthritis (OA) and embedded in atelocollagen gels. As a control, chondrocytes were isolated and embedded in gels in the same manner. These composites were cultured for 3 weeks, and cell proliferation and matrix production were evaluated using histology and immunochemistry. RESULTS: Histologically, minced cartilage showed cell migration from the cartilage fragments into the gel, with the Bern score and cell count in the minced cartilage group being significantly higher than those in the control group. Immunohistochemistry revealed that the number of Ki67-positive cells, the expression of LECT-1 and TGF-β, and the glycosaminoglycan content were significantly higher in the minced cartilage than in the control group. Minced cartilage exhibited superior cell migration, proliferation, and glycosaminoglycan content than isolated chondrocytes. CONCLUSION: Our findings support that minced cartilage has a favorable potential for cell proliferation and matrix production compared with the isolated chondrocytes after enzymatic treatment.
Authors: J A Buckwalter; S L Woo; V M Goldberg; E C Hadley; F Booth; T R Oegema; D R Eyre Journal: J Bone Joint Surg Am Date: 1993-10 Impact factor: 5.284
Authors: Davide Edoardo Bonasia; James A Martin; Antongiulio Marmotti; Richard L Amendola; Joseph A Buckwalter; Roberto Rossi; Davide Blonna; Huston Davis Adkisson; Annunziato Amendola Journal: Am J Sports Med Date: 2011-08-09 Impact factor: 6.202
Authors: Ning Wang; Sibylle Grad; Martin J Stoddart; Philipp Niemeyer; Kilian Reising; Hagen Schmal; Norbert P Südkamp; Mauro Alini; Gian M Salzmann Journal: Int Orthop Date: 2013-11-28 Impact factor: 3.075
Authors: S Muraki; H Oka; T Akune; A Mabuchi; Y En-yo; M Yoshida; A Saika; T Suzuki; H Yoshida; H Ishibashi; S Yamamoto; K Nakamura; H Kawaguchi; N Yoshimura Journal: Osteoarthritis Cartilage Date: 2009-04-17 Impact factor: 6.576
Authors: Clara Levinson; Emma Cavalli; Dolman Mostafa Sindi; Benjamin Kessel; Marcy Zenobi-Wong; Stefan Preiss; Gian Salzmann; Philipp Neidenbach Journal: Orthop J Sports Med Date: 2019-09-10