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Literature DB >> 3031021

Localization of a genetic region involved in McrB restriction by Escherichia coli K-12.

Abstract

A 5,500-base-pair BglII-EcoRI fragment proximal to the hsd genes of Escherichia coli K-12 has been cloned in the plasmid vector pUC9. The resultant hybrid plasmid was shown to complement the mcrB mutation of E. coli K802. The presence of the hybrid plasmid in strain K802 caused an 18.3-fold drop in transformation efficiency with AluI-methylated pACYC184 relative to unmethylated pACYC184. These results indicate that the cloned DNA is involved in the McrB system restriction of 5-methylcytosine DNA.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1987 PMID： 3031021 PMCID： PMC212013 DOI： 10.1128/jb.169.4.1757-1759.1987

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

15 in total

1. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.

Authors: A C Chang; S N Cohen
Journal: J Bacteriol Date: 1978-06 Impact factor: 3.490

2. Transformation in Escherichia coli: cryogenic preservation of competent cells.

Authors: D A Morrison
Journal: J Bacteriol Date: 1977-10 Impact factor: 3.490

Review 3. DNA modification and restriction.

Authors: W Arber
Journal: Prog Nucleic Acid Res Mol Biol Date: 1974

5. Nucleotide sequence of the McrB region of Escherichia coli K-12 and evidence for two independent translational initiation sites at the mcrB locus.

Authors: T K Ross; E C Achberger; H D Braymer
Journal: J Bacteriol Date: 1989-04 Impact factor: 3.490

6. Identification and characterization of a gene responsible for inhibiting propagation of methylated DNA sequences in mcrA mcrB1 Escherichia coli strains.

Authors: P L Kretz; S W Kohler; J M Short
Journal: J Bacteriol Date: 1991-08 Impact factor: 3.490

10. Identification of a second polypeptide required for McrB restriction of 5-methylcytosine-containing DNA in Escherichia coli K12.

Authors: T K Ross; E C Achberger; H D Braymer
Journal: Mol Gen Genet Date: 1989-04

Localization of a genetic region involved in McrB restriction by Escherichia coli K-12.

1. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.

2. Transformation in Escherichia coli: cryogenic preservation of competent cells.

Review 3. DNA modification and restriction.

4. Mitomycin C-induced expression of trpA of Salmonella typhimurium inserted into the plasmid ColE1.

5. Escherichia coli promoter sequences predict in vitro RNA polymerase selectivity.

6. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

7. Sequence diversity among related genes for recognition of specific targets in DNA molecules.

Review 8. DNA restriction and modification mechanisms in bacteria.

9. Escherichia coli K-12 restricts DNA containing 5-methylcytosine.

10. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Review 1. Nucleoside triphosphate-dependent restriction enzymes.

2. The McrBC restriction endonuclease assembles into a ring structure in the presence of G nucleotides.

3. Nomenclature relating to restriction of modified DNA in Escherichia coli.

4. Overproduction and purification of McrC protein from Escherichia coli K-12.

5. Nucleotide sequence of the McrB region of Escherichia coli K-12 and evidence for two independent translational initiation sites at the mcrB locus.

6. Identification and characterization of a gene responsible for inhibiting propagation of methylated DNA sequences in mcrA mcrB1 Escherichia coli strains.

7. Cloning and structural characterization of the mcrA locus of Escherichia coli.

8. McrBs, a modulator peptide for McrBC activity.

Review 9. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

10. Identification of a second polypeptide required for McrB restriction of 5-methylcytosine-containing DNA in Escherichia coli K12.