Literature DB >> 3029095

Purification of the receptor for platelet-derived growth factor from porcine uterus.

L Rönnstrand, M P Beckmann, B Faulders, A Ostman, B Ek, C H Heldin.   

Abstract

The receptor for platelet-derived growth factor has been purified to homogeneity on a large scale from porcine uterus. The purification procedure utilizes solubilization of uterus membranes by Triton X-100, followed by sequential chromatographies on wheat germ agglutinin-Sepharose, fast protein liquid chromatography Mono-Q, and anti-phosphotyrosine-Sepharose. About 160 micrograms of homogeneous and functionally active 170-kDa receptor could be purified from 5 kg of uterus tissue. The pure receptor responded to platelet-derived growth factor stimulation by autophosphorylation, indicating that the receptor has a kinase domain as an integral part of the molecule. A rabbit antiserum was produced against the pure receptor, which specifically recognizes the intact 170-kDa receptor.

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Year:  1987        PMID： 3029095

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  19 in total

Review 1.  Molecular aspects of pathological processes in the artery wall.

Authors:  J W van Neck; H P Bloemers
Journal:  Mol Biol Rep       Date:  1992-11       Impact factor: 2.316

2.  Platelet-derived growth factor (PDGF)-dependent association of phospholipase C-gamma with the PDGF receptor signaling complex.

Authors:  D K Morrison; D R Kaplan; S G Rhee; L T Williams
Journal:  Mol Cell Biol       Date:  1990-05       Impact factor: 4.272

3.  Deletion of the kinase insert sequence of the platelet-derived growth factor beta-receptor affects receptor kinase activity and signal transduction.

Authors:  L Severinsson; B Ek; K Mellström; L Claesson-Welsh; C H Heldin
Journal:  Mol Cell Biol       Date:  1990-02       Impact factor: 4.272

4.  cDNA cloning and expression of the human A-type platelet-derived growth factor (PDGF) receptor establishes structural similarity to the B-type PDGF receptor.

Authors:  L Claesson-Welsh; A Eriksson; B Westermark; C H Heldin
Journal:  Proc Natl Acad Sci U S A       Date:  1989-07       Impact factor: 11.205

5.  Biosynthesis and intracellular transport of the receptor for platelet-derived growth factor.

Authors:  L Claesson-Welsh; L Rönnstrand; C H Heldin
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

6.  The A-type receptor for platelet-derived growth factor mediates protein tyrosine phosphorylation, receptor transmodulation and a mitogenic response.

Authors:  A Hammacher; M Nistér; C H Heldin
Journal:  Biochem J       Date:  1989-11-15       Impact factor: 3.857

7.  Characterization of a novel anti-peptide antibody that recognizes a specific conformation of the platelet-derived growth factor receptor.

Authors:  S Bishayee; S Majumdar; C D Scher; S Khan
Journal:  Mol Cell Biol       Date:  1988-09       Impact factor: 4.272

8.  cDNA cloning and expression of a human platelet-derived growth factor (PDGF) receptor specific for B-chain-containing PDGF molecules.

Authors:  L Claesson-Welsh; A Eriksson; A Morén; L Severinsson; B Ek; A Ostman; C Betsholtz; C H Heldin
Journal:  Mol Cell Biol       Date:  1988-08       Impact factor: 4.272

9.  Inhibition of glycosylation processing alters the growth parameters of cells transformed by the oncogene of simian sarcoma virus.

Authors:  A Hadwiger-Fangmeier; H Niemann; T Tamura
Journal:  Arch Virol       Date:  1989       Impact factor: 2.574

10.  Aberrant expression of receptors for platelet-derived growth factor in an anaplastic thyroid carcinoma cell line.

Authors:  N E Heldin; B Gustavsson; L Claesson-Welsh; A Hammacher; J Mark; C H Heldin; B Westermark
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205
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