Literature DB >> 3027117

An ELISA for the detection of rhinovirus specific antibody in serum and nasal secretion.

W S Barclay, W Al-Nakib.   

Abstract

An enzyme-linked immunosorbent assay (ELISA) which detects rhinovirus specific antibody in human sera and nasal secretions, has been developed. This sandwich ELISA utilizes a rabbit antirhinovirus hyperimmune serum as the capture antibody and was found to be very sensitive, detecting rhinovirus specific antibody in the serum at dilutions of 1:10(6) and 1:10(3.5) for IgG and IgA immunoglobulins, respectively. Thus, this new assay is 10(2)-10(4) times more sensitive than our standard neutralization test. Furthermore, this increase in sensitivity has enabled us to reliably detect rhinovirus specific immunoglobulins in unconcentrated nasal washings, which are thought to be particularly important for protection against rhinovirus reinfection. A preliminary study of the immune response in human volunteers challenged with rhinovirus using this new ELISA system is presented and further applications and potential of the method are also discussed.

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Year:  1987        PMID： 3027117      PMCID： PMC7119497          DOI： 10.1016/0166-0934(87)90048-6

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  10 in total

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Authors:  J P Fox; M K Cooney; C E Hall
Journal:  Am J Epidemiol       Date:  1975-02       Impact factor: 4.897

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Journal:  Antimicrob Agents Chemother       Date:  1985-05       Impact factor: 5.191

3.  Antigenic determinants of infective and inactivated human rhinovirus type 2.

Authors:  K Lonberg-Holm; F H Yin
Journal:  J Virol       Date:  1973-07       Impact factor: 5.103

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Authors:  T R Cate; R D Rossen; R G Douglas; W T Butler; R B Couch
Journal:  Am J Epidemiol       Date:  1966-09       Impact factor: 4.897

5.  Solid phase antibody assay by means of enzyme conjugated to anti-immunoglobulin.

Authors:  P Leinikki; S Pässilä
Journal:  J Clin Pathol       Date:  1976-12       Impact factor: 3.411

6.  Diagnosis of parainfluenza virus infection in children and older patients by detection of specific IgM antibody.

Authors:  J T van der Logt; A M van Loon; F W Heessen; J van der Veen
Journal:  J Med Virol       Date:  1985-06       Impact factor: 2.327

7.  Enzyme-linked immunosorbent assay for detection of antibody in volunteers experimentally infected with human coronavirus strain 229 E.

Authors:  C A Kraaijeveld; S E Reed; M R Macnaughton
Journal:  J Clin Microbiol       Date:  1980-10       Impact factor: 5.948

8.  Hemagglutination-inhibition test in rhinovirus infections of volunteers.

Authors:  S E Reed; T S Hall
Journal:  Infect Immun       Date:  1973-07       Impact factor: 3.441

9.  Use of the enzyme-linked immunosorbent assay to detect serum antibody responses of volunteers who received attenuated influenza A virus vaccines.

Authors:  B R Murphy; E L Tierney; B A Barbour; R H Yolken; D W Alling; H P Holley; R E Mayner; R M Chanock
Journal:  Infect Immun       Date:  1980-08       Impact factor: 3.441

10.  Alteration in antibody reactivity with foot-and-mouth disease virus (FMDV) 146S antigen before and after binding to a solid phase or complexing with specific antibody.

Authors:  K C McCullough; J R Crowther; R N Butcher
Journal:  J Immunol Methods       Date:  1985-09-03       Impact factor: 2.303
  10 in total
  8 in total

1.  The time course of the humoral immune response to rhinovirus infection.

Authors:  W S Barclay; W al-Nakib; P G Higgins; D A Tyrrell
Journal:  Epidemiol Infect       Date:  1989-12       Impact factor: 2.451

2.  Smoking, alcohol consumption, and susceptibility to the common cold.

Authors:  S Cohen; D A Tyrrell; M A Russell; M J Jarvis; A P Smith
Journal:  Am J Public Health       Date:  1993-09       Impact factor: 9.308

3.  Signs and symptoms in common colds.

Authors:  D A Tyrrell; S Cohen; J E Schlarb
Journal:  Epidemiol Infect       Date:  1993-08       Impact factor: 2.451

4.  Some recent work at the Common Cold Unit, Salisbury.

Authors:  D A Tyrrell
Journal:  Infection       Date:  1988 Sep-Oct       Impact factor: 3.553

Review 5.  Some advances in the diagnosis of respiratory virus infections.

Authors:  D A Tyrrell
Journal:  J R Coll Physicians Lond       Date:  1987-07

6.  Evaluation of an enzyme-linked immunosorbent assay that measures rhinovirus-specific antibodies in human sera and nasal secretions.

Authors:  W S Barclay; K A Callow; M Sergeant; W al-Nakib
Journal:  J Med Virol       Date:  1988-08       Impact factor: 2.327

Review 7.  Epidemiology, pathogenesis, and treatment of the common cold.

Authors:  R B Turner
Journal:  Ann Allergy Asthma Immunol       Date:  1997-06       Impact factor: 6.347

Review 8.  Microfluidic-based virus detection methods for respiratory diseases.

Authors:  E Alperay Tarim; Betul Karakuzu; Cemre Oksuz; Oyku Sarigil; Melike Kizilkaya; Mahmoud Khatib A A Al-Ruweidi; Huseyin Cagatay Yalcin; Engin Ozcivici; H Cumhur Tekin
Journal:  Emergent Mater       Date:  2021-03-25
  8 in total

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