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Literature DB >> 3025869

Phosphorylation by protein kinase C of a 20-kDa cytoskeletal polypeptide enhances its susceptibility to digestion by calpain.

S Pontremoli, E Melloni, M Michetti, B Sparatore, F Salamino, O Sacco, B L Horecker.

Abstract

Incubation of the cytoskeletal fraction from human neutrophils with the proteolytically activated form of protein kinase C results in the phosphorylation of several components, including a 20-kDa polypeptide, probably consisting of myosin light chains. The 20-kDa polypeptide is also specifically phosphorylated by activated protein kinase C in a solubilized 20-kDa/80-kDa complex that was obtained after sonication of the insoluble cytoskeletal fraction. Phosphorylation of this polypeptide, in either the insoluble cytoskeletal fraction or the soluble 20-kDa/80-kDa complex, greatly enhances its susceptibility to digestion by the Ca2+-requiring proteinase (calpain, EC 3.4.22.17) of human neutrophils. Thus, signals that activate calpain by mobilizing intracellular calcium would lead to proteolytic activation of protein kinase C, phosphorylation of cytoskeletal proteins, and remodeling of the cytoskeleton by proteolysis of at least one cytoskeletal component.

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Year: 1987 PMID： 3025869 PMCID： PMC304214 DOI： 10.1073/pnas.84.2.398

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

37 in total

1. Binding of protein kinase C to neutrophil membranes in the presence of Ca2+ and its activation by a Ca2+-requiring proteinase.

Authors: E Melloni; S Pontremoli; M Michetti; O Sacco; B Sparatore; F Salamino; B L Horecker
Journal: Proc Natl Acad Sci U S A Date: 1985-10 Impact factor: 11.205

Review 2. The phorbol ester receptor: a phospholipid-regulated protein kinase.

Authors: C L Ashendel
Journal: Biochim Biophys Acta Date: 1985-09-09

3. Regulation of fructose-1,6-bisphosphatase in yeast by phosphorylation/dephosphorylation.

Authors: D Müller; H Holzer
Journal: Biochem Biophys Res Commun Date: 1981-12-15 Impact factor: 3.575

Review 4. Contractile proteins in leukocyte function.

Authors: F S Southwick; T P Stossel
Journal: Semin Hematol Date: 1983-10 Impact factor: 3.851

5. The involvement of calpain in the activation of protein kinase C in neutrophils stimulated by phorbol myristic acid.

Authors: E Melloni; S Pontremoli; M Michetti; O Sacco; B Sparatore; B L Horecker
Journal: J Biol Chem Date: 1986-03-25 Impact factor: 5.157

6. Phorbol ester-induced phosphorylation of a transmembrane glycoprotein (GP 180) in human blood platelets.

Authors: L Y Bourguignon; G Walker; G J Bourguignon
Journal: J Biol Chem Date: 1985-09-25 Impact factor: 5.157

7. A model for intracellular translocation of protein kinase C involving synergism between Ca2+ and phorbol esters.

Authors: M Wolf; H LeVine; W S May; P Cuatrecasas; N Sahyoun
Journal: Nature Date: 1985 Oct 10-16 Impact factor: 49.962

8. Phorbol myristate acetate mediates redistribution of protein kinase C in human neutrophils: potential role in the activation of the respiratory burst enzyme.

Authors: M Wolfson; L C McPhail; V N Nasrallah; R Snyderman
Journal: J Immunol Date: 1985-09 Impact factor: 5.422

9. Reversible activation of human neutrophil calpain promoted by interaction with plasma membranes.

Authors: S Pontremoli; B Sparatore; F Salamino; M Michetti; O Sacco; E Melloni
Journal: Biochem Int Date: 1985-07

10. Effects of chemotactic factors and other agents on the amounts of actin and a 65,000-mol-wt protein associated with the cytoskeleton of rabbit and human neutrophils.

Authors: R Yassin; J Shefcyk; J R White; W Tao; M Volpi; T F Molski; P H Naccache; M B Feinstein; R I Sha'afi
Journal: J Cell Biol Date: 1985-07 Impact factor: 10.539

9 in total

1. Respiratory burst oxidase and three of four oxidase-related polypeptides are associated with the cytoskeleton of human neutrophils.

Authors: R C Woodman; J M Ruedi; A J Jesaitis; N Okamura; M T Quinn; R M Smith; J T Curnutte; B M Babior
Journal: J Clin Invest Date: 1991-04 Impact factor: 14.808

2. Phosphorylation and proteolytic modification of specific cytoskeletal proteins in human neutrophils stimulated by phorbol 12-myristate 13-acetate.

Authors: S Pontremoli; E Melloni; M Michetti; B Sparatore; F Salamino; O Sacco; B L Horecker
Journal: Proc Natl Acad Sci U S A Date: 1987-06 Impact factor: 11.205

9. Binding of monoclonal antibody AA4 to gangliosides on rat basophilic leukemia cells produces changes similar to those seen with Fc epsilon receptor activation.

Authors: C Oliver; N Sahara; S Kitani; A R Robbins; L M Mertz; R P Siraganian
Journal: J Cell Biol Date: 1992-02 Impact factor: 10.539