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Literature DB >> 2831536

An endogenous activator of the Ca2+-dependent proteinase of human neutrophils that increases its affinity for Ca2+.

S Pontremoli¹, E Melloni, M Michetti, F Salamino, B Sparatore, B L Horecker.

Abstract

An endogenous activator of the Ca2+-dependent proteinase (calpain) has been identified in human neutrophils. In the presence of the activator, the affinity of calpain for Ca2+ is increased by greater than 100-fold and maximum catalytic activity is observed with Ca2+ concentration below 1 microM. The activator is a heat-stable protein having an apparent molecular mass of approximately equal to 40 kDa. It appears to be associated with the cytoskeletal fraction of human neutrophils. Neutrophils also contain an endogenous cytosolic calpain inhibitor (calpastatin), which is readily separated from the activator by size-exclusion chromatography. The effects of the activator and inhibitor appear to be antagonistic and may constitute a physiological mechanism for modulating intracellular calpain activity.

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Year: 1988 PMID： 2831536 PMCID： PMC279854 DOI： 10.1073/pnas.85.6.1740

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

25 in total

1. A NEUTRAL, CALCIUM-ACTIVATED PROTEINASE FROM THE SOLUBLE FRACTION OF RAT BRAIN.

Authors: G GUROFF
Journal: J Biol Chem Date: 1964-01 Impact factor: 5.157

2. Studies of a calcium-activated neutral protease from chicken skeletal muscle. I. Purification and characterization.

Authors: S Ishiura; H Murofushi; K Suzuki; K Imahori
Journal: J Biochem Date: 1978-07 Impact factor: 3.387

3. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors: M M Bradford
Journal: Anal Biochem Date: 1976-05-07 Impact factor: 3.365

4. Binding to erythrocyte membrane is the physiological mechanism for activation of Ca2+-dependent neutral proteinase.

Authors: S Pontremoli; E Melloni; B Sparatore; F Salamino; M Michetti; O Sacco; B L Horecker
Journal: Biochem Biophys Res Commun Date: 1985-04-16 Impact factor: 3.575

5. Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range.

Authors: S Udenfriend; S Stein; P Böhlen; W Dairman; W Leimgruber; M Weigele
Journal: Science Date: 1972-11-24 Impact factor: 47.728

6. Ca2+-dependent neutral proteinase from human erythrocytes: activation by Ca2+ ions and substrate and regulation by the endogenous inhibitor.

Authors: E Melloni; F Salamino; B Sparatore; M Michetti; S Pontremoli
Journal: Biochem Int Date: 1984-04

7. Two cytosolic, Ca2+-dependent, neutral proteinases from rabbit liver: purification and properties of the proenzymes.

Authors: E Melloni; S Pontremoli; F Salamino; B Sparatore; M Michetti; B L Horecker
Journal: Arch Biochem Biophys Date: 1984-08-01 Impact factor: 4.013

8. Purification and characterization of calcium-dependent proteases from rat heart.

Authors: D E Croall; G N DeMartino
Journal: J Biol Chem Date: 1983-05-10 Impact factor: 5.157

9. Activation by hemoglobin of the Ca2+-requiring neutral proteinase of human erythrocytes: structural requirements.

Authors: S Pontremoli; B Sparatore; E Melloni; M Michetti; B L Horecker
Journal: Biochem Biophys Res Commun Date: 1984-08-30 Impact factor: 3.575

10. The cytosol of human erythrocytes contains a highly Ca2+-sensitive thiol protease (calpain I) and its specific inhibitor protein (calpastatin).

Authors: T Murakami; M Hatanaka; T Murachi
Journal: J Biochem Date: 1981-12 Impact factor: 3.387

15 in total

1. Activation of calpain I in thrombin-stimulated platelets is regulated by the initial elevation of the cytosolic Ca2+ concentration.

Authors: H Ishii; Y Suzuki; M Kuboki; M Morikawa; M Inoue; M Kazama
Journal: Biochem J Date: 1992-06-15 Impact factor: 3.857

2. Nonselective autophagy of cytosolic enzymes by isolated rat hepatocytes.

Authors: J Kopitz; G O Kisen; P B Gordon; P Bohley; P O Seglen
Journal: J Cell Biol Date: 1990-09 Impact factor: 10.539

3. Independence of neutrophil respiratory burst oxidant generation from the early cytosolic calcium response after stimulation with unopsonized Candida albicans hyphae.

Authors: D R Wysong; C A Lyman; R D Diamond
Journal: Infect Immun Date: 1989-05 Impact factor: 3.441

An endogenous activator of the Ca2+-dependent proteinase of human neutrophils that increases its affinity for Ca2+.

1. A NEUTRAL, CALCIUM-ACTIVATED PROTEINASE FROM THE SOLUBLE FRACTION OF RAT BRAIN.

2. Studies of a calcium-activated neutral protease from chicken skeletal muscle. I. Purification and characterization.

3. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

4. Binding to erythrocyte membrane is the physiological mechanism for activation of Ca2+-dependent neutral proteinase.

5. Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range.

6. Ca2+-dependent neutral proteinase from human erythrocytes: activation by Ca2+ ions and substrate and regulation by the endogenous inhibitor.

7. Two cytosolic, Ca2+-dependent, neutral proteinases from rabbit liver: purification and properties of the proenzymes.

8. Purification and characterization of calcium-dependent proteases from rat heart.

9. Activation by hemoglobin of the Ca2+-requiring neutral proteinase of human erythrocytes: structural requirements.

10. The cytosol of human erythrocytes contains a highly Ca2+-sensitive thiol protease (calpain I) and its specific inhibitor protein (calpastatin).

1. Activation of calpain I in thrombin-stimulated platelets is regulated by the initial elevation of the cytosolic Ca2+ concentration.

2. Nonselective autophagy of cytosolic enzymes by isolated rat hepatocytes.

3. Independence of neutrophil respiratory burst oxidant generation from the early cytosolic calcium response after stimulation with unopsonized Candida albicans hyphae.

Review 4. High molecular mass intracellular proteases.

5. Site-directed activation of calpain is promoted by a membrane-associated natural activator protein.

6. PEST motifs are not required for rapid calpain-mediated proteolysis of c-fos protein.

7. Redox-Dependent Calpain Signaling in Airway and Pulmonary Vascular Remodeling in COPD.

8. Calcium dependent thiol protease caldonopain and its specific endogenous inhibitor in Leishmania donovani.

9. Cell surface topology creates high Ca2+ signalling microdomains.

10. Reovirus-induced apoptosis is preceded by increased cellular calpain activity and is blocked by calpain inhibitors.