Özlen Güzel-Akdemir1, Andrea Angeli2, Kübra Demir1, Claudiu T Supuran2, Atilla Akdemir3. 1. a Department of Pharmaceutical Chemistry, Faculty of Pharmacy , Istanbul University , Istanbul , Turkey. 2. b Department of NEUROFARBA , Sezione di Scienze Farmaceutiche Universita degli Studi di Firenze , Sesto Fiorentino , Florence , Italy. 3. c Computer-Aided Drug Discovery Laboratory, Department of Pharmacology, Faculty of Pharmacy , Bezmialem Vakif University , Istanbul , Turkey.
Abstract
A small collection of 26 structurally novel thiazolidinone-containing compounds, without the well-known sulphonamide zinc-binding group, were synthesised and tested in enzyme inhibition assays against the tumour-associated hCA IX enzyme. Inhibition constants in the lower micromolar region (KI < 25 μM) have been measured for 17 of the 26 compounds. Even though the KI values are relatively weak, the fact that they do not contain a sulphonamide moiety suggests that these compounds do not interact with the active site zinc ion. Therefore, docking studies and molecular dynamics simulations have been performed to suggest binding poses for these structurally novel inhibitors.
A small collection of 26 structurally novel thiazolidinone-containing compounds, without the well-known sulphonamide zinc-binding group, were synthesised and tested in enzyme inhibition assays against the tumour-associated hCA IX enzyme. Inhibition constants in the lower micromolar region (KI < 25 μM) have been measured for 17 of the 26 compounds. Even though the KI values are relatively weak, the fact that they do not contain a sulphonamide moiety suggests that these compounds do not interact with the active site zinc ion. Therefore, docking studies and molecular dynamics simulations have been performed to suggest binding poses for these structurally novel inhibitors.
Carbonic anhydrases (CAs; EC 4.2.1.1) are a structurally diverse family of metallo-enzymes that catalyse the reversible hydration of carbon dioxide to bicarbonate and protons. Due to this physiologically important reaction, CAs are important in controlling pH values and supplying bicarbonate ions for various processes. The humanCA isoform IX (hCA IX), which belongs to the α-subfamily of CAs (αCAs), is expressed in the stomach and peritoneal lining. More interestingly, hCA IX is upregulated in many solid hypoxic tumours and it helps the tumourcell to function in an acidic environment. This provides an advantage over healthy cells in the tumour microenvironment. As such, the development of selective hCA IX inhibitors may provide novel compounds for the cancerchemotherapy.Many inhibitors of hCA IX has been synthesised and tested in the last decade, including substituted-phenylacetamido aromatic sulphonamides, open saccharin analogues, probenecid analogues, and isatin analogues. Many CA inhibitors (including hCA IX inhibitors) bind to the active site zinc ion via a so called zinc-binding group (ZBG), which is a sulphonamide in most cases, and thereby block the reversible hydration of carbon dioxide. One such example is compound SLC-0111, a sulphonamide-containing potent CA inhibitor, which is currently in clinical trials. Other binding modes of inhibitors are also possible, including binding to allosteric sites and binding to the active site but without a direct interaction with the zinc ion.Recently, we focussed our attention to the 4-thiazolidinone moiety, which in general provides good safety profiles, bioavailabilities, and various biological activities. Several studies report that thiazolidinone-based agents show diverse pharmacological properties including antifungal, antiparasitic, antimicrobial, antioxidant, anticonvulsant, anti-HIV, anti-inflammatory, anti-tuberculosis, and anti-tumour activities.In this work, we synthesised and tested 26 structurally novel thiazolidinone-containing compounds, without the well-known sulphonamide ZBG, in enzyme inhibition assays against hCA IX. Inhibition constants were obtained in the lower micromolar region. Subsequently, docking studies and molecular dynamics simulations were performed to suggest binding poses for these compounds.
Materials and methods
Chemistry
All reagents and solvents were purchased from Sigma-Aldrich (Darmstadt, Germany) and used without further purification. Melting points were measured in open capillary tubes with a Buchi 530 melting point apparatus and are uncorrected. IR (KBr) spectra were recorded using a Perkin-Elmer 1600 FTIR spectrophotometer. 1H-NMR, HSQC, and C-NMR (proton decoupled) spectra were recorded on a VarianUNITYINOVA 500 MHz spectrometer and Agilent VNMRS 600 MHz NMR spectrometer. Elemental analyses were performed on a Carlo Erba Model 1106 elemental analyser. Mass spectra (LC/MS-APCI) were recorded on a FinniganTM LCQTM Mass Spectrometer in the negative ionisation mode.
(RS)-2-hydroxy-2-phenylacetohydrazide (1),
(RS)-methyl 2-hydroxy-2-phenylacetate (0.05 mol) and 12 ml hydrazine hydrate (98%) were heated under reflux for 12 h. The reaction mixture was transferred to a crystallising dish and left aside until crystallisation. The crude product thus obtained was recrystallised from ethanol.
General procedure for synthesis of (RS)-1–(2-hydroxy-2-phenylacetyl)-4-substituted thiosemicarbazides (2)
To a solution of 0.005 mol 1 in 30 ml EtOH, 0.005 mol of an appropriate isothiocyanate were added. The resulting mixture was heated under reflux for 3 h. After cooling, the precipitate was separated and purified by washing with hot EtOH or recrystallisation from EtOH.
General procedure for synthesis of (RS)-N'-[3–(4-substitutedphenyl)-4-oxo-1,3-thiazolidin-2-ylidene]-2-hydroxy-2-phenylacetohydrazide (3)/(RS)-N'-[3–(4-substitutedphenyl)-5-methyl-4-oxo-1,3-thiazolidin-2-ylidene]-2-hydroxy-2-phenylacetohydrazide (4)
A mixture of 0.005 mol 2, 0.005 mol of ethyl α-bromoacetate/ethyl α-bromopropionate, and 0.02 mol of fused sodium acetate in 25 ml anhydrous EtOH was heated under reflux for 3 h. The reaction mixture was cooled, diluted with water, and allowed to stand overnight. The precipitate was filtered, dried, and recrystallised from EtOH.
A stopped-flow instrument (SX.18 MV-R Applied Photophysics model) was used for assaying the CA-catalysed CO2 hydration activity. Inhibitor and enzyme were pre-incubated for 15 min for allowing the complete formation of the enzyme-inhibitor adduct. IC50 values were obtained from dose response curves working at seven different concentrations of test compound (from 0.1 nM to 50 µM), by fitting the curves using PRISM (www.graphpad.com) and non-linear least squares methods, the obtained values representing the mean of at least three different determinations. The inhibition constants (KI) were derived from the IC50 values by using the Cheng-Prusoff equation, as follows: KI = IC50/(1 + [S]/Km) where [S] represents the CO2concentration at which the measurement was carried out, and Km the concentration of substrate at which the enzyme activity is at half maximal. All enzymes used were recombinant, produced in Escherichia coli as reported earlier. The concentration of hCA IX used in the assay was 12.1 nM.
Preparation of compound series 3 and 4 for docking studies
The three-dimensional structures of all ligands, including all possible stereoisomers, were prepared (MOE software package, v2016.08, Chemical Computing Group, Inc, Montreal, Canada). Afterwards, the ligands were energy minimised using the MMFF94x force field.
Preparation of hCA IX structure
The crystal structure of the catalytic domain of hCA IX in complex with acetazolamide (pdb: 3iai; 2.20 Å) was obtained from the RCSB protein databank. The structure was protonated using the Protonate3D tool of the MOE software package and subsequently the obtained structure was energy-minimised using the AMBER12:EHT force field. The protein atoms of subunit A and the corresponding active site zinc ion were retained and all other atoms were omitted.
Docking protocol
The GOLD Suite software package (v5.6.1, CCDC, Cambridge, UK) and the ChemScore scoring function were used to dock the compounds into the hCA IX structures (25 dockings per ligand). The binding pocket was defined as all residues within 13 Å of a centroid corresponding to the location of the acetazolamideC2 atom. The best three docked poses were retained for each ligand.
Molecular dynamics simulations
All molecular dynamics simulations were performed using the NAMD software package (v2.12, Theoretical and Computational Biophysics group, NIH Center for Macromolecular Modeling and Bioinformatics, The Beckman Institute, University of Illinois at Urbana-Champaign). The select docked poses (ligand-enzyme complexes) were first placed into the centre of a box with periodic boundary conditions (minimal distance of 10 Å between protein and boundary). Afterwards, both water molecules (Tip3) and counter ions (NaCl) were added to generate a solvated and neutral system. After a steepest-descent energy minimisation (AMBER12:EHT), the system was first heated from 0 to 300 K during 100 ps followed by an 100 ps equilibration simulation (position restraints on all protein and ligand heavy atoms). Finally, the system was simulated for 1 ns at constant temperature (300 K, Langevin, default values) and pressure (1 bar, Nosé-Hoover Langevin, default values), without any position restrains. The only restraints applied were distance restraints to keep the zinc ion in the correct orientation towards His94, His96, and His119 (distance restraints between Zn and N atom of histidine: 1.8 Å; default settings). The timestep was set to 0.002 fs and all bonds were constrained using the ShakeH algorithm.
Results and discussion
The chemical synthesis of 3a-o and 4a-k compounds is outlined in Scheme 1. The synthesis of several intermediate thiosemicarbazide derivatives except 2g and 2h were previously reported elsewhere,. 4-Thiazolidinones were prepared starting from 2-hydroxy-2-phenylacetohydrazide (1) which afforded intermediate thiosemicarbazides (2) on reaction with aryl isothiocyanates. The thiosemicarbazides in turn furnished 3 and 4 with ethyl α-bromoacetate and ethyl α-bromopropionate, respectively.
Scheme 1.
General synthesis of 3a-o and 4a-k. Reagents and conditions: (i) hydrazine hydrate, EtOH, reflux, 6 h; (ii) EtOH, reflux, 3 h; and (iii) sodium acetate, anhydrous EtOH, reflux, 3 h.
General synthesis of 3a-o and 4a-k. Reagents and conditions: (i) hydrazine hydrate, EtOH, reflux, 6 h; (ii) EtOH, reflux, 3 h; and (iii) sodium acetate, anhydrous EtOH, reflux, 3 h.The structures of 2g, 2h, 3a-o, and 4a-k were confirmed by analytical and spectral (IR, 1HNMR, C-NMR (proton decoupled), HSQC-2D, and LCMS-APCI) data.The IR spectra exhibited O-H/N-H and C=O bands in the 3226–3567 cm−1 and 1684–1705 cm−1 regions attributed to the common CONH functions of 2, 3, and 4,. Observation of new endocyclicC=O bands (1718–1766 cm−1) characteristic for such structures besides C=O amide bands (1684–1705 cm−1) in the IR spectra of 3 and 4 supported the aimed cyclisation,.The 1H-NMR spectra of 3 and 4 displayed two singlets and two quartettes attributed to the methylene (SCH2) and methane (SCHCH3) ring protons at 5-position of the 4-thiazolidinone system at about δ 4.13–4.24 and 4.39–4.53 ppm, respectively. The C-OH and CONH protons were observed at about δ 6.46–6.81 and δ 10.81–11.55 ppm, respectively,.HSQC 2D NMR experiments of compounds 3f, 3m, 4g, and 4k allowed explicit assignments for the proton and carbonchemical shifts. The spectra substantiated the expected conversion and revealed the typical 4-thiazolidinoneC5 (compound 3), and C5 (compound 4) resonances at δ 30.48–30.63, and 40.11–40.48 ppm, respectively. Existence of cross peaks connecting C5 (δ 30.48–30.63 ppm) with the singlet at δ 4.13–4.18 ppm provided evidence for unambiguous assignment for compounds 3f and 3m. Existence of cross peaks connecting C5-CH3 (δ 19.74–21.65 ppm) with the doublet at δ 1.53–1.55 ppm and C5 (δ 40.11–40.48 ppm) with the quartette at δ 4.41–4.49 ppm allowed for unambiguous assignment for compounds 4g and 4k. CNMR data of compounds 3d, 3f, 3m, 3o, 4c, 4e, 4g, and 4k also support the structure of 4-thiazolidinone ring via C2, C4, and C5 resonances that appeared at 150.30–153.75, 170.50–171.63, and 29.76–30.05 ppm (19.10–19.26 ppm for compound 4), respectively. The CONH resonance of compounds 3 and 4 was observed at δ 168.22–170.80 ppm,.In the mass spectra of 3c and 4g (M-H)− peaks were observed which confirmed their molecular weights. Further spectral details have already been presented in the “Materials and methods” section.
hCA IX enzyme inhibition studies
A small collection of 26 thiazolidinonecompounds have been tested in enzyme inhibition assays against hCA IX and 17 compounds were identified with an inhibition constant (KI) lower than 25 μM (Table 1). A subset of five compounds show KI values lower than 10 μM (3d: 1.4 μM; 3f: 1.2 μM; 3o: 1.1 μM; 4c: 2.4 μM; 4e: 7.7 μM). The measured KI values for these ligands are at least 44-fold lower compared to the KI value of the potent hCA inhibitor acetazolamide (25 nM). However, it should be noted that these compounds do not contain a sulphonamide moiety that functions as a ZBG, which is present in many inhibitors of hCAs.
Table 1.
Inhibition constants (KI; μM) for hCA IX of compound series 3 and 4.
3,4
cmp
R1
R2
hCA IX
3a
2-F
H
22.8
3b
3-F
H
>100
3c
4-F
H
18.2
3d
2-Cl
H
1.4
3e
3-Cl
H
13.2
3f
4-Cl
H
1.2
3g
2-Br
H
>100
3h
3-Br
H
17.3
3i
4-Br
H
>100
3j
2-CH3
H
>100
3k
3-CH3
H
13.7
3l
4-CH3
H
>100
3m
4-OCH3
H
20.4
3n
4-NO2
H
22.5
3o
3-CF3
H
1.1
4a
3-F
CH3
16.7
4b
4-F
CH3
11.4
4c
3-Cl
CH3
2.4
4d
4-Cl
CH3
>100
4e
3-Br
CH3
7.7
4f
4-Br
CH3
12.5
4g
3-CH3
CH3
>100
4h
4-CH3
CH3
>100
4i
4-OCH3
CH3
>100
4j
4-NO2
CH3
21.3
4k
3-CF3
CH3
17.3
AAZ
–
–
0.025
Mean from three different assays, by a stopped flow technique (errors were in the range of ±5–10% of the reported values).
Inhibition constants (KI; μM) for hCA IX of compound series 3 and 4.Mean from three different assays, by a stopped flow technique (errors were in the range of ±5–10% of the reported values).It is difficult to draw conclusive structure-activity relationships from these data since the KI values are relatively close to each other.Compounds 3n and 4j show KI values of 22.5 and 21.3 μM, respectively. These two compounds have nitro groups on the para positions of their phenyl moieties. This may indicate the presence of a direct interaction between the nitrooxygen atoms and the zinc ion of the hCA IX active site. The other compounds may form interactions with the zinc ion via their hydroxyl or carbonyl groups.Docking studies and molecular dynamics simulations have been performed to suggest possible binding poses for these compounds.
Docking studies into the active site of hCA IX
All 26 thiazolidinone-containing molecules were docked into the active site of hCA IX, with and without a zinc-bound water molecule.Docking studies without a zinc-bound water molecule suggests that only compounds with para-nitro substituents (compounds 3n and 4j; pose 1) may interact directly with the active site zinc ion (Figure 1). The carbonyl group of the thiazolidinone ring forms a hydrogen bond with the side chain of Gln92, while the other carbonyl group forms a hydrogen bond to the side chain of Trp5. The unsubstituted phenyl group forms hydrophobic interactions with the side chain of Pro202. An additional hydrogen bond is formed between the hydroxyl group (R isomer) with the backbone of Ser3. In the S isomer, this hydroxyl group may interact with the backbone carbonyl group of Pro201 or point to the solvent. The methyl group at the R2 position (R isomer) may form a hydrophobic interaction with the side chain of Val131.
Figure 1.
Representation of docking pose 1, showing compound 4j (purple; OH in R isomer; turquoise OH in S isomer) in the active site of hCA IX. Hydrogen bonds and interactions to the Zn2+-ion are depicted in red dashed lines. A similar docked pose has been obtained for compound 3n.
Representation of docking pose 1, showing compound 4j (purple; OH in R isomer; turquoise OH in S isomer) in the active site of hCA IX. Hydrogen bonds and interactions to the Zn2+-ion are depicted in red dashed lines. A similar docked pose has been obtained for compound 3n.The docking studies with a zinc-bound water molecule in the active site suggest docked poses in which the ligand interacts directly with the water molecule (pose 2; Figure 2).
Figure 2.
Representation of docking pose 2, showing compound 3d (turquoise; OH in R isomer; purple OH in S isomer) in the active site of hCA IX. Hydrogen bonds and interactions to the Zn2+-ion are depicted in red dashed lines.
Representation of docking pose 2, showing compound 3d (turquoise; OH in R isomer; purple OH in S isomer) in the active site of hCA IX. Hydrogen bonds and interactions to the Zn2+-ion are depicted in red dashed lines.In pose 2, the unsubstituted phenyl moiety forms a hydrophobic interaction with the side chain of His94 (Figure 2). The carbonyl group forms a hydrogen bond to the side chain of Gln92 and the substituted phenyl ring is located in a hydrophobic pocket formed by Leu91, Val121, Val131, Leu135, Leu141, and Leu198. The ligands sulphur atom may form interactions with the backbone carbonyl of Pro201. The thiazolidinonecarbonyl group is solvent exposed. The hydroxyl group of the ligand in the R isomer (turquoise, Figure 2) forms a direct hydrogen bond with the zinc-bound water molecule, while this is not observed for the hydroxyl group in the S isomer (purple, Figure 2). However, the distance and orientation of this hydroxyl group are such that it might be possible after slight conformational changes in this area.Docking pose 2, with the hydroxyl group in either S or R orientation, was observed for all compounds 3 and 4, as the hydrophobiccavity formed by Leu91, Val121, Val131, Leu135, Leu141, and Leu198 seems to be large enough to accept the substituted phenyl group of the ligand.Molecular dynamics simulations have been performed to investigate whether the suggested docked poses 1 and 2 would be stable during molecular dynamics simulations. To this end, the ligand-enzyme complex was first placed into a box with periodic boundary conditions. Afterwards, both water molecules and counter ions (NaCl) were added to generate a solvated and neutral system. After a steepest-descent energy minimisation (AMBER12:EHT), the system was first heated from 0 to 300 K during 100 ps followed by a 100-ps equilibration simulation (position restraints on all protein and ligand heavy atoms). Finally, the system was simulated for 1 ns at constant temperature (300 K) and pressure (1 bar), without any position restrains. The only restraints applied were dihedral and distance restraints to keep the zinc ion in the correct orientation towards His94, His96, and His119.
MD simulation of hCA IX-4j complex (pose 1)
The simulation of compound 4j (with hydroxyl in R isomer) in the active site of hCA IX (docked pose 1; purple; Figure 1) indicates that the interaction between the oxygen atom of the nitro group and the zinc ion is not stable. The direct interaction is lost and a water molecule comes in between (Figure 3). The newly obtained pose of compound 4j is remarkably similar to its original docked pose (0 ps), with the exception of the bridging water molecule and the absence of an interaction with Gln92 (Figure 3). Hydrogen bonds are being formed with the side chain of Trp5 (84.3% of 1 ns simulation) and Thr199 (81.8% of 1 ns simulation) and the backbone of Ser3 (65.4% of 1 ns simulation).
Figure 3.
Snapshot of docking pose 1 after a 1 ns MD simulation, showing compound 4j (OH in R isomer) in the active site of hCA IX. Hydrogen bonds and interactions to the Zn2+-ion are depicted in red dashed lines.
Snapshot of docking pose 1 after a 1 ns MD simulation, showing compound 4j (OH in R isomer) in the active site of hCA IX. Hydrogen bonds and interactions to the Zn2+-ion are depicted in red dashed lines.A similar result has been observed for the molecular dynamics simulation of compound 4j with the hydroxyl group in the S isomer (turquoise; Figure 1). Again the nitro group forms interactions with a zinc-bound water molecule and the backbone of Thr199 (89.2% of 1 ns simulation) or Thr200 (9.2% of 1 ns simulation). In addition, hydrogen bonds are formed with the backbone of Ser3 (12.2% of 1 ns simulation) and the side chain of Trp5 (86.1% of 1 ns simulation).
MD simulation of hCA IX-3d complex (pose 2)
The simulation of compound 3d (R) reveals that the hydrogen bond between the hydroxyl group of the ligand (R isomer) and the zinc-bound water molecule is not stable and this bond is lost very early in the simulation (Figure 4). New hydrogen bonds are formed between the ligand and the backbone of Thr199 (69.3% of 1 ns simulation) or the side chain of Thr200 (45.0% of 1 ns simulation). An additional hydrogen bond is formed between the ligand and the side chain of Gln92 (16.0% of 1 ns simulation). One of the hydrogen atoms of the zinc-bound water molecule projects to the centroid of the unsubstituted phenyl moiety of the ligand. In addition, this unsubstituted phenyl group forms hydrophobic interactions with His94. The substituted phenyl group is located in the hydrophobic pocket formed by the side chains of Leu91, Val121, Val131, and Leu141. The two carbonyl groups and the sulphur atom form hydrogen bonds with other water molecules that have entered the active site.
Figure 4.
Snapshot of docking pose 1 after a 1 ns MD simulation, showing compound 3d (OH in R isomer) in the active site of hCA IX. Hydrogen bonds and interactions to the Zn2+-ion are depicted in red dashed lines.
Snapshot of docking pose 1 after a 1 ns MD simulation, showing compound 3d (OH in R isomer) in the active site of hCA IX. Hydrogen bonds and interactions to the Zn2+-ion are depicted in red dashed lines.The start conformation for the simulation of compound 3d (hydroxyl in S isomer; Figure 2, purple) indicates that no hydrogen bond between the ligand and the zinc-bound water molecule is formed. The hydrogen bond with the side chain of Gln92 seems to be stable (97.7% of 1 ns simulation), whereas the interaction between the ligand sulphur and the backbone carbonyl of Pro201 is less frequently observed (4.6% of 1 ns simulation). Occasionally, the ligand forms a hydrogen bond with the side chain of Thr200 (15.7% of 1 ns simulation). The substituted phenyl group of the ligand is located in the hydrophobic pocket lined by Leu91, Val121, Val131, and Leu141 during the whole simulation.
Conclusions
Thiazolidinone-containing compounds, without the well-known sulphonamide ZBG, inhibit the tumour-associated hCA IX enzyme with KI values in the lower micromolar range. Docking studies in combination with molecular dynamics simulations suggested several binding poses for these compounds, in which the ligand form no direct interactions with the active site zinc ion. These structurally novel compounds, without the well-known sulphonamide ZBG, may be interesting candidates as novel classes as CAIs.
Authors: Antonio Mastrolorenzo; Stefano Rusconi; Andrea Scozzafava; Giuseppe Barbaro; Claudiu T Supuran Journal: Curr Med Chem Date: 2007 Impact factor: 4.530
Authors: Alice Kunzler; Patrícia D Neuenfeldt; Adriana M das Neves; Claudio M P Pereira; Gabriela H Marques; Patrícia S Nascente; Maureen H V Fernandes; Silvia O Hübner; Wilson Cunico Journal: Eur J Med Chem Date: 2013-03-26 Impact factor: 6.514
Authors: Kerim Babaoglu; Mark A Page; Victoria C Jones; Michael R McNeil; Changjiang Dong; James H Naismith; Richard E Lee Journal: Bioorg Med Chem Lett Date: 2003-10-06 Impact factor: 2.823
Authors: Erik O Pettersen; Peter Ebbesen; Roben G Gieling; Kaye J Williams; Ludwig Dubois; Philippe Lambin; Carol Ward; James Meehan; Ian H Kunkler; Simon P Langdon; Anne H Ree; Kjersti Flatmark; Heidi Lyng; Maria J Calzada; Luis Del Peso; Manuel O Landazuri; Agnes Görlach; Hubert Flamm; Jochen Kieninger; Gerald Urban; Andreas Weltin; Dean C Singleton; Syed Haider; Francesca M Buffa; Adrian L Harris; Andrea Scozzafava; Claudiu T Supuran; Isabella Moser; Gerhard Jobst; Morten Busk; Kasper Toustrup; Jens Overgaard; Jan Alsner; Jacques Pouyssegur; Johanna Chiche; Nathalie Mazure; Ibtissam Marchiq; Scott Parks; Afshan Ahmed; Margaret Ashcroft; Silvia Pastorekova; Yihai Cao; Kasper M Rouschop; Brad G Wouters; Marianne Koritzinsky; Hilda Mujcic; Dan Cojocari Journal: J Enzyme Inhib Med Chem Date: 2014-10-27 Impact factor: 5.051
Authors: Mohammad M Al-Sanea; Ahmed Elkamhawy; Sora Paik; Silvia Bua; So Ha Lee; Mohamed A Abdelgawad; Eun Joo Roh; Wagdy M Eldehna; Claudiu T Supuran Journal: J Enzyme Inhib Med Chem Date: 2019-12 Impact factor: 5.051
Scheme 1.
Figure 1.
Figure 2.
Figure 3.
Figure 4.