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Literature DB >> 302439

The quantitation of fluorescence by photography.

Abstract

A method based on theory has been developed for the photographic quantitation of fluorescent substances. DNA stained with ethidium in agarose gels is used as an example of an application of this method. In the course of developing this method we have demonstrated that the empirical methods employed by others authors can give rise to large systematic errors. We have also developed an approximate method based on photographic theory, avoiding the use of digital integration which is required by the rigorous method.

Entities: Chemical

Mesh：

Substances：

Year: 1977 PMID： 302439 PMCID： PMC343764 DOI： 10.1093/nar/4.5.1409

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

11 in total

1. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.

Authors: R A Laskey; A D Mills
Journal: Eur J Biochem Date: 1975-08-15

2. Action of nicking-closing enzyme on supercoiled and nonsupercoiled closed circular DNA: formation of a Boltzmann distribution of topological isomers.

Authors: D E Pulleyblank; M Shure; D Tang; J Vinograd; H P Vosberg
Journal: Proc Natl Acad Sci U S A Date: 1975-11 Impact factor: 11.205

3. Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structures.

Authors: A Dugaiczyk; H W Boyer; H M Goodman
Journal: J Mol Biol Date: 1975-07-25 Impact factor: 5.469

4. Location of histones on simian virus 40 DNA.

Authors: B Polisky; B McCarthy
Journal: Proc Natl Acad Sci U S A Date: 1975-08 Impact factor: 11.205

5. Conformational fluctuations of DNA helix.

Authors: D E Depew; J C Wang
Journal: Proc Natl Acad Sci U S A Date: 1975-11 Impact factor: 11.205

6. The number of superhelical turns in native virion SV40 DNA and minicol DNA determined by the band counting method.

Authors: M Shure; J Vinograd
Journal: Cell Date: 1976-06 Impact factor: 41.582

7. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors: P A Sharp; B Sugden; J Sambrook
Journal: Biochemistry Date: 1973-07-31 Impact factor: 3.162

8. A fluorescence assay for DNA with covalently linked complementary sequences.

Authors: A R Morgan; V Paetkau
Journal: Can J Biochem Date: 1972-02

9. A map of the sites on bacteriophage PM2 DNA for the restriction endonucleases HindIII and HpaII.

Authors: C Brack; H Eberle; T A Bickle; R Yuan
Journal: J Mol Biol Date: 1976-06-14 Impact factor: 5.469

10. Electrophoretic analysis of covalently closed SV40 DNA: Boltzmann distributions of DNA species.

Authors: R J DeLeys; D A Jackson
Journal: Nucleic Acids Res Date: 1976-03 Impact factor: 16.971

13 in total

1. Deletion analysis of the F plasmid oriT locus.

Authors: Y H Fu; M M Tsai; Y N Luo; R C Deonier
Journal: J Bacteriol Date: 1991-02 Impact factor: 3.490

2. Quantitative analysis of electrophoresis data: novel curve fitting methodology and its application to the determination of a protein-DNA binding constant.

Authors: S E Shadle; D F Allen; H Guo; W K Pogozelski; J S Bashkin; T D Tullius
Journal: Nucleic Acids Res Date: 1997-02-15 Impact factor: 16.971

10. Mapping the in vivo arrangement of nucleosomes on simian virus 40 chromatin by the photoaddition of radioactive hydroxymethyltrimethylpsoralen.

Authors: G W Robinson; L M Hallick
Journal: J Virol Date: 1982-01 Impact factor: 5.103

The quantitation of fluorescence by photography.

1. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.

2. Action of nicking-closing enzyme on supercoiled and nonsupercoiled closed circular DNA: formation of a Boltzmann distribution of topological isomers.

3. Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structures.

4. Location of histones on simian virus 40 DNA.

5. Conformational fluctuations of DNA helix.

6. The number of superhelical turns in native virion SV40 DNA and minicol DNA determined by the band counting method.

7. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

8. A fluorescence assay for DNA with covalently linked complementary sequences.

9. A map of the sites on bacteriophage PM2 DNA for the restriction endonucleases HindIII and HpaII.

10. Electrophoretic analysis of covalently closed SV40 DNA: Boltzmann distributions of DNA species.

1. Deletion analysis of the F plasmid oriT locus.

2. Quantitative analysis of electrophoresis data: novel curve fitting methodology and its application to the determination of a protein-DNA binding constant.

3. In vitro recombination of bacteriophage T7 DNA detected by a direct physical assay.

4. DNA double-strand breaks and alkali-labile bonds produced by bleomycin.

5. Nucleotide sequence of a highly repetitive component of rat DNA.

6. Chromosomal initiation in Bacillus subtilis may involve two closely linked origins.

7. The effect of Escherichia coli Uvr protein binding on the topology of supercoiled DNA.

8. Yeast RNA polymerase I binds preferentially to A+T-rich linkers in rDNA.

9. The distribution of quinacrine on chromosomes as determined by X-ray microanalysis. I. Q-bands on CHO chromosomes.

10. Mapping the in vivo arrangement of nucleosomes on simian virus 40 chromatin by the photoaddition of radioactive hydroxymethyltrimethylpsoralen.