Michelly Pires Queiroz1, Martiniano da Silva Lima2, Marília Ferreira Frazão Tavares de Melo2, Camila Carolina de Menezes Santos Bertozzo2, Daline Fernandes de Araújo3, Gerlane Coelho Bernardo Guerra3, Rita de Cassia Ramos do Egypto Queiroga4, Juliana Késsia Barbosa Soares5. 1. Program of Food Science and Tecnology, Federal University of Paraiba, Cidade Universitária, s/n - Castelo Branco III, João Pessoa, PB 58051-085, Brazil. Electronic address: queiroz_m.p@hotmail.com. 2. Laboratory of Experimental Nutrition, Department of Nutrition, Federal University of Campina Grande, Cuité, CG, Brazil. 3. Department of Biofísica and Pharmacology, Federal University of Rio Grande do Norte, Natal, RN, Brazil. 4. Program of Food Science and Tecnology, Federal University of Paraiba, Cidade Universitária, s/n - Castelo Branco III, João Pessoa, PB 58051-085, Brazil; Laboratory of Bromatology, Department of Nutrition, Federal University of Paraiba, João Pessoa, PB, Brazil. Electronic address: rcqueiroga@uol.com.br. 5. Program of Food Science and Tecnology, Federal University of Paraiba, Cidade Universitária, s/n - Castelo Branco III, João Pessoa, PB 58051-085, Brazil; Laboratory of Experimental Nutrition, Department of Nutrition, Federal University of Campina Grande, Cuité, CG, Brazil.
Abstract
BACKGROUND: Maternal consumption of fatty acids can alter neuronal membrane function, synaptic connections, and protect the brain from alterations caused by disturbances such as lipid peroxidation and anxiety in the offspring. We aimed to investigate how the maternal consumption of conjugated linoleic acid (CLA) interferes in anxiety behavior of the offspring and cerebral lipid peroxidation. METHODS: Three groups were formed: control (CG) - diet without CLA; CLA1 - diet containing 1% of CLA; and CLA3 - diet containing 3% of CLA. These diets were offered to the mothers from the 7th day of gestation until the end of lactation. The following behavioral tests were used: Elevated plus maze (EPM), Open Field (OF) and Light-dark Box (LDB). Levels of malondialdehyde (MDA) and glutathione were measured in the offspring's brains. Data were analyzed by ANOVA followed by the Holm-Sidak post-test or the Kruskal-Wallis test (p < 0.05). RESULTS: CLA1 and CLA3 showed higher number of entries in the open arms and time spent in the central area in EPM, they translocated and ambulated more in the clear area of the LDB and presented more rearing in the OF compared to CG (p < 0.05); moreover, they presented higher concentration of glutathione and lower MDA in brain tissue (p < 0.05). LIMITATIONS: We evaluated the effect of maternal consumption of CLA on anxiety and lipid peroxidation in rats' offspring, but a similar study should be performed in humans. CONCLUSIONS: Maternal intake of CLA induced a decrease in the parameters of anxiety and cerebral lipid peroxidation in the offspring.
BACKGROUND: Maternal consumption of fatty acids can alter neuronal membrane function, synaptic connections, and protect the brain from alterations caused by disturbances such as lipid peroxidation and anxiety in the offspring. We aimed to investigate how the maternal consumption of conjugated linoleic acid (CLA) interferes in anxiety behavior of the offspring and cerebral lipid peroxidation. METHODS: Three groups were formed: control (CG) - diet without CLA; CLA1 - diet containing 1% of CLA; and CLA3 - diet containing 3% of CLA. These diets were offered to the mothers from the 7th day of gestation until the end of lactation. The following behavioral tests were used: Elevated plus maze (EPM), Open Field (OF) and Light-dark Box (LDB). Levels of malondialdehyde (MDA) and glutathione were measured in the offspring's brains. Data were analyzed by ANOVA followed by the Holm-Sidak post-test or the Kruskal-Wallis test (p < 0.05). RESULTS:CLA1 and CLA3 showed higher number of entries in the open arms and time spent in the central area in EPM, they translocated and ambulated more in the clear area of the LDB and presented more rearing in the OF compared to CG (p < 0.05); moreover, they presented higher concentration of glutathione and lower MDA in brain tissue (p < 0.05). LIMITATIONS: We evaluated the effect of maternal consumption of CLA on anxiety and lipid peroxidation in rats' offspring, but a similar study should be performed in humans. CONCLUSIONS: Maternal intake of CLA induced a decrease in the parameters of anxiety and cerebral lipid peroxidation in the offspring.
Authors: Lorenz S Neuwirth; Michael T Verrengia; Zachary I Harikinish-Murrary; Jessica E Orens; Oscar E Lopez Journal: Front Mol Neurosci Date: 2022-08-17 Impact factor: 6.261