| Literature DB >> 30232692 |
Hitoshi Takemae1, Chaerul Basri2, Ni Luh Putu Ika Mayasari2, Ronald Tarigan1,2, Hiroshi Shimoda3, Tsutomu Omatsu4, Didik Pramono2, Danang Dwi Cahyadi2, Ryosuke Kobayashi1, Keisuke Iida1, Tetsuya Mizutani4, Ken Maeda3, Srihadi Agungpriyono2, Eiichi Hondo5.
Abstract
Flying foxes belonging to the genus Pteropus are known to be reservoirs of zoonotic viruses. In this study, we describe the isolation of Pteropine orthoreovirus (PRV) from rectal swab samples of Pteropus vampyrus in Indonesia. PRV is an emerging zoonotic respiratory virus that can be transmitted from bats to humans. Rectal swabs (n = 91) were screened by PCR for PRV and 10 (11%) were positive. Phylogenetic analysis based on nucleotide sequences indicated that the S2, S3, S4, M3, L2, and L3 segments of one isolate (Garut-69) were closely related to previously isolated strains in Indonesia. The remaining gene segments showed both similarity and genetic divergence with other PRV strains, suggesting that re-assortment events had occurred. This is the first report of PRV infection to P. vampyrus in West Java, Indonesia.Entities:
Keywords: Indonesia; PCR; Phylogenetic analysis; Pteropine orthoreovirus; Pteropus vampyrus
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Year: 2018 PMID： 30232692 DOI： 10.1007/s11262-018-1603-y
Source DB: PubMed Journal: Virus Genes ISSN： 0920-8569 Impact factor: 2.332