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Literature DB >> 3022239

Tn7 transposition: a multigene process. Identification of a regulatory gene product.

Abstract

Tn7 transposition is abolished by the deletion of a 2.2kb HindIII fragment from the central region of the transposon. Transposition is restored when the fragment is present in trans. When this fragment is present in trans with wild-type Tn7, transposition frequencies are stimulated 10-100-fold. The DNA sequence of this fragment has been determined and found to contain one long open reading frame coding for a protein of molecular weight 61 187. We have visualised this protein using a DNA-directed prokaryotic transcription-translation system. This gene may fill a regulatory role in the mechanism of Tn7 transposition. When present in trans the 2.2kb HindIII fragment alleviates a transcriptional block of promoter activity detected in Tn7.

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Year: 1986 PMID： 3022239 PMCID： PMC311824 DOI： 10.1093/nar/14.20.7915

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

21 in total

1. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.

Authors: A C Chang; S N Cohen
Journal: J Bacteriol Date: 1978-06 Impact factor: 3.490

2. Genetic analysis of Tn7 transposition.

Authors: A Ouartsi; D Borowski; J Brevet
Journal: Mol Gen Genet Date: 1985

3. In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals.

Authors: M J Casadaban; J Chou; S N Cohen
Journal: J Bacteriol Date: 1980-08 Impact factor: 3.490

4. Transposition of a deoxyribonucleic acid sequence encoding trimethoprim and streptomycin resistances from R483 to other replicons.

Authors: P T Barth; N Datta; R W Hedges; N J Grinter
Journal: J Bacteriol Date: 1976-03 Impact factor: 3.490

3. Structural and functional analysis of Tn4430: identification of an integrase-like protein involved in the co-integrate-resolution process.

Authors: J Mahillon; D Lereclus
Journal: EMBO J Date: 1988-05 Impact factor: 11.598

3 in total

Tn7 transposition: a multigene process. Identification of a regulatory gene product.

1. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.

2. Genetic analysis of Tn7 transposition.

3. In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals.

4. Transposition of a deoxyribonucleic acid sequence encoding trimethoprim and streptomycin resistances from R483 to other replicons.

5. Massive airway hemorrhage after transtracheal aspiration.

6. Transposition of Tn7 in Pseudomonas aeruginosa and isolation of alk::Tn7 mutations.

7. A system for shotgun DNA sequencing.

8. Trans-complementable copy-number mutants of plasmid ColE1.

9. DNA sequencing with chain-terminating inhibitors.

10. Mutagenesis by insertion of drug resistance transposon Tn7 into a vibrio species.

1. Purification and characterization of TnsC, a Tn7 transposition protein that binds ATP and DNA.

2. DNA sequence analysis of five genes; tnsA, B, C, D and E, required for Tn7 transposition.

3. Structural and functional analysis of Tn4430: identification of an integrase-like protein involved in the co-integrate-resolution process.