The past decade has witnessed an explosion in the use of super-resolution fluorescence microscopy methods in biology and other fields. Single-molecule localization microscopy (SMLM) is one of the most widespread of these methods and owes its success in large part to the ability to control the on-off state of fluorophores through various chemical, photochemical, or binding-unbinding mechanisms. We provide here a comprehensive overview of switchable fluorophores in SMLM including a detailed review of all major classes of SMLM fluorophores, and we also address strategies for labeling specimens, considerations for multichannel and live-cell imaging, potential pitfalls, and areas for future development.
The past decade has witnessed an explopan class="Chemical">sion in the use of super-resolution fluorescence microscopy methods in biology and other fields. Single-molecule localization microscopy (SMLM) is one of the most widespread of these methods and owes its success in large part to the ability to control the on-off state of fluorophores through various chemical, photochemical, or binding-unbinding mechanisms. We provide here a comprehensive overview of switchable fluorophores in SMLM including a detailed review of all major classes of SMLM fluorophores, and we also address strategies for labeling specimens, considerations for multichannel and live-cell imaging, potential pitfalls, and areas for future development.
Authors: X Michalet; F F Pinaud; L A Bentolila; J M Tsay; S Doose; J J Li; G Sundaresan; A M Wu; S S Gambhir; S Weiss Journal: Science Date: 2005-01-28 Impact factor: 47.728
Authors: Alexandros Pertsinidis; Konark Mukherjee; Manu Sharma; Zhiping P Pang; Sang Ryul Park; Yunxiang Zhang; Axel T Brunger; Thomas C Südhof; Steven Chu Journal: Proc Natl Acad Sci U S A Date: 2013-07-02 Impact factor: 11.205
Authors: Leila Nahidiazar; Alexandra V Agronskaia; Jorrit Broertjes; Bram van den Broek; Kees Jalink Journal: PLoS One Date: 2016-07-08 Impact factor: 3.240
Authors: Elena A Protasova; Alexander S Mishin; Konstantin A Lukyanov; Eugene G Maksimov; Alexey M Bogdanov Journal: Photochem Photobiol Sci Date: 2021-06-04 Impact factor: 3.982
Authors: Jun Chen; Katriel E Cho; Daria Skwarzynska; Shaylyn Clancy; Nicholas J Conley; Sarah M Clinton; Xiaokun Li; Li Lin; J Julius Zhu Journal: J Neurosci Date: 2021-02-24 Impact factor: 6.167
Authors: Paula K Zhu; W Sharon Zheng; Peng Zhang; Miao Jing; Philip M Borden; Farhan Ali; Kaiming Guo; Jiesi Feng; Jonathan S Marvin; Yali Wang; Jinxia Wan; Li Gan; Alex C Kwan; Li Lin; Loren L Looger; Yulong Li; Yajun Zhang Journal: Nano Lett Date: 2020-05-12 Impact factor: 11.189