Literature DB >> 3022015

Construction and properties of a viable herpes simplex virus 1 recombinant lacking coding sequences of the alpha 47 gene.

P Mavromara-Nazos, M Ackermann, B Roizman.   

Abstract

The five alpha genes, alpha 0, alpha 4, alpha 22, alpha 27, and alpha 47, are the first set of herpes simplex virus 1 genes to be transcribed and expressed in productively infected cells. We report here the construction of a viral recombinant from which all of the coding sequences of the alpha 47 gene were deleted. In addition to the alpha 47 protein, infected cell lysates did not contain detectable amounts of two polypeptide bands with apparent molecular weights of 18,000 and 21,000 which could be specified by a gene whose regulatory domain and 5' transcribed noncoding sequences overlap with the coding sequences of the alpha 47 gene. The alpha 47- virus grew as well as the wild-type parent virus in Vero, baby hamster kidney, and Rat-1 cell lines.

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Year:  1986        PMID： 3022015      PMCID： PMC288963     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  34 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Anatomy of herpes simplex virus (HSV) DNA. X. Mapping of viral genes by analysis of polypeptides and functions specified by HSV-1 X HSV-2 recombinants.

Authors:  L S Morse; L Pereira; B Roizman; P A Schaffer
Journal:  J Virol       Date:  1978-05       Impact factor: 5.103

3.  A generalized technique for deletion of specific genes in large genomes: alpha gene 22 of herpes simplex virus 1 is not essential for growth.

Authors:  L E Post; B Roizman
Journal:  Cell       Date:  1981-07       Impact factor: 41.582

4.  Molecular engineering of the herpes simplex virus genome: insertion of a second L-S junction into the genome causes additional genome inversions.

Authors:  E S Mocarski; L E Post; B Roizman
Journal:  Cell       Date:  1980-11       Impact factor: 41.582

5.  Cloning of reiterated and nonreiterated herpes simplex virus 1 sequences as BamHI fragments.

Authors:  L E Post; A J Conley; E S Mocarski; B Roizman
Journal:  Proc Natl Acad Sci U S A       Date:  1980-07       Impact factor: 11.205

6.  Fine-structure mapping and functional analysis of temperature-sensitive mutants in the gene encoding the herpes simplex virus type 1 immediate early protein VP175.

Authors:  R A Dixon; P A Schaffer
Journal:  J Virol       Date:  1980-10       Impact factor: 5.103

7.  Genomic location and lack of phosphorylation of the HSV immediate-early polypeptide IE 12.

Authors:  H S Marsden; J Lang; A J Davison; R G Hope; D M MacDonald
Journal:  J Gen Virol       Date:  1982-09       Impact factor: 3.891

8.  Structural features of the herpes simplex virus alpha gene 4, 0, and 27 promoter-regulatory sequences which confer alpha regulation on chimeric thymidine kinase genes.

Authors:  S Mackem; B Roizman
Journal:  J Virol       Date:  1982-12       Impact factor: 5.103

9.  A herpes simplex virus type 1 function continuously required for early and late virus RNA synthesis.

Authors:  R J Watson; J B Clements
Journal:  Nature       Date:  1980-05-29       Impact factor: 49.962

10.  Regulation of alpha genes of herpes simplex virus: expression of chimeric genes produced by fusion of thymidine kinase with alpha gene promoters.

Authors:  L E Post; S Mackem; B Roizman
Journal:  Cell       Date:  1981-05       Impact factor: 41.582
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  32 in total

1.  Multiple immediate-early gene-deficient herpes simplex virus vectors allowing efficient gene delivery to neurons in culture and widespread gene delivery to the central nervous system in vivo.

Authors:  C E Lilley; F Groutsi; Z Han; J A Palmer; P N Anderson; D S Latchman; R S Coffin
Journal:  J Virol       Date:  2001-05       Impact factor: 5.103

2.  Site specificity of the inhibitory effects of oligo(nucleoside methylphosphonate)s complementary to the acceptor splice junction of herpes simplex virus type 1 immediate early mRNA 4.

Authors:  M Kulka; C C Smith; L Aurelian; R Fishelevich; K Meade; P Miller; P O Ts'o
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

3.  The regions important for the activator and repressor functions of herpes simplex virus type 1 alpha protein ICP27 map to the C-terminal half of the molecule.

Authors:  M A Hardwicke; P J Vaughan; R E Sekulovich; R O'Conner; R M Sandri-Goldin
Journal:  J Virol       Date:  1989-11       Impact factor: 5.103

4.  Oncolytic herpes simplex virus vector with enhanced MHC class I presentation and tumor cell killing.

Authors:  T Todo; R L Martuza; S D Rabkin; P A Johnson
Journal:  Proc Natl Acad Sci U S A       Date:  2001-05-15       Impact factor: 11.205

5.  Suppression of the phenotype of gamma(1)34.5- herpes simplex virus 1: failure of activated RNA-dependent protein kinase to shut off protein synthesis is associated with a deletion in the domain of the alpha47 gene.

Authors:  B He; J Chou; R Brandimarti; I Mohr; Y Gluzman; B Roizman
Journal:  J Virol       Date:  1997-08       Impact factor: 5.103

6.  The herpes simplex virus 1 RNA binding protein US11 is a virion component and associates with ribosomal 60S subunits.

Authors:  R J Roller; B Roizman
Journal:  J Virol       Date:  1992-06       Impact factor: 5.103

7.  UL34, the target of the herpes simplex virus U(S)3 protein kinase, is a membrane protein which in its unphosphorylated state associates with novel phosphoproteins.

Authors:  F C Purves; D Spector; B Roizman
Journal:  J Virol       Date:  1992-07       Impact factor: 5.103

8.  ONCOLYTIC HERPES SIMPLEX VIRUS 1 (HSV-1) VECTORS: INCREASING TREATMENT EFFICACY AND RANGE THROUGH STRATEGIC VIRUS DESIGN.

Authors:  J Carson; D Haddad; M Bressman; Y Fong
Journal:  Drugs Future       Date:  2010       Impact factor: 0.148

9.  Infection of polarized MDCK cells with herpes simplex virus 1: two asymmetrically distributed cell receptors interact with different viral proteins.

Authors:  A E Sears; B S McGwire; B Roizman
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-15       Impact factor: 11.205

10.  Synergistic antiviral activities of oligonucleoside methylphosphonates complementary to herpes simplex virus type 1 immediate-early mRNAs 4, 5, and 1.

Authors:  M Kulka; C C Smith; J Levis; R Fishelevich; J C Hunter; C D Cushman; P S Miller; P O Ts'o; L Aurelian
Journal:  Antimicrob Agents Chemother       Date:  1994-04       Impact factor: 5.191
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