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Literature DB >> 3022012

Production of guanidine-resistant and -dependent poliovirus mutants from cloned cDNA: mutations in polypeptide 2C are directly responsible for altered guanidine sensitivity.

Abstract

cDNA fragments representing the region in polypeptide 2C containing mutations in a guanidine-resistant or -dependent mutant were cloned into the wild-type background of an infectious clone. Transfection of COS-1 cells with these plasmids yielded viruses that were either completely resistant to 2.0 mM guanidine hydrochloride or dependent on this concentration of drug for growth.

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Year: 1986 PMID： 3022012 PMCID： PMC288960

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

24 in total

1. Studies of the inhibitory action of guanidine on poliovirus multiplication in cell cultures.

Authors: D CROWTHER; J L MELNICK
Journal: Virology Date: 1961-09 Impact factor: 3.616

2. In vitro phenotypic markers of a poliovirus recombinant constructed from infectious cDNA clones of the neurovirulent Mahoney strain and the attenuated Sabin 1 strain.

Authors: M Kohara; T Omata; A Kameda; B L Semler; H Itoh; E Wimmer; A Nomoto
Journal: J Virol Date: 1985-03 Impact factor: 5.103

3. A poliovirus temperature-sensitive RNA synthesis mutant located in a noncoding region of the genome.

Authors: P Sarnow; H D Bernstein; D Baltimore
Journal: Proc Natl Acad Sci U S A Date: 1986-02 Impact factor: 11.205

4. A chimeric plasmid from cDNA clones of poliovirus and coxsackievirus produces a recombinant virus that is temperature-sensitive.

Authors: B L Semler; V H Johnson; S Tracy
Journal: Proc Natl Acad Sci U S A Date: 1986-03 Impact factor: 11.205

5. Construction of poliovirus intertypic recombinants by use of cDNA.

Authors: G Stanway; P J Hughes; G D Westrop; D M Evans; G Dunn; P D Minor; G C Schild; J W Almond
Journal: J Virol Date: 1986-03 Impact factor: 5.103

6. Recombinants of Mahoney and Sabin strain poliovirus type 1: analysis of in vitro phenotypic markers and evidence that resistance to guanidine maps in the nonstructural proteins.

Authors: E A Emini; J Leibowitz; D C Diamond; J Bonin; E Wimmer
Journal: Virology Date: 1984-08 Impact factor: 3.616

6. Synchronous replication of poliovirus RNA: initiation of negative-strand RNA synthesis requires the guanidine-inhibited activity of protein 2C.

Authors: D J Barton; J B Flanegan
Journal: J Virol Date: 1997-11 Impact factor: 5.103

Production of guanidine-resistant and -dependent poliovirus mutants from cloned cDNA: mutations in polypeptide 2C are directly responsible for altered guanidine sensitivity.

1. Studies of the inhibitory action of guanidine on poliovirus multiplication in cell cultures.

2. In vitro phenotypic markers of a poliovirus recombinant constructed from infectious cDNA clones of the neurovirulent Mahoney strain and the attenuated Sabin 1 strain.

3. A poliovirus temperature-sensitive RNA synthesis mutant located in a noncoding region of the genome.

4. A chimeric plasmid from cDNA clones of poliovirus and coxsackievirus produces a recombinant virus that is temperature-sensitive.

5. Construction of poliovirus intertypic recombinants by use of cDNA.

6. Recombinants of Mahoney and Sabin strain poliovirus type 1: analysis of in vitro phenotypic markers and evidence that resistance to guanidine maps in the nonstructural proteins.

7. Systematic nomenclature of picornavirus proteins.

8. Guanidine-selected mutants of poliovirus: mapping of point mutations to polypeptide 2C.

9. Poliovirus mutant that does not selectively inhibit host cell protein synthesis.

10. Guanidine-resistant poliovirus mutants produce modified 37-kilodalton proteins.

1. Genetic and biochemical studies of poliovirus cis-acting replication element cre in relation to VPg uridylylation.

2. Initiation of poliovirus negative-strand RNA synthesis requires precursor forms of p2 proteins.

3. Gliotoxin: inhibitor of poliovirus RNA synthesis that blocks the viral RNA polymerase 3Dpol.

4. cis-acting lesions targeted to the hydrophobic domain of a poliovirus membrane protein involved in RNA replication.

5. Strand-specific RNA synthesis defects in a poliovirus with a mutation in protein 3A.

6. Synchronous replication of poliovirus RNA: initiation of negative-strand RNA synthesis requires the guanidine-inhibited activity of protein 2C.

7. Poliovirus 2C region functions during encapsidation of viral RNA.

8. Guanidine-resistant mutants of poliovirus have distinct mutations in peptide 2C.

9. Poliovirus protein 3AB forms a complex with and stimulates the activity of the viral RNA polymerase, 3Dpol.

10. Dipyridamole reversibly inhibits mengovirus RNA replication.