Literature DB >> 3021627

DNA sequence and product analysis of the virF locus responsible for congo red binding and cell invasion in Shigella flexneri 2a.

T Sakai, C Sasakawa, S Makino, M Yoshikawa.   

Abstract

The DNA sequence of virF, a locus associated with virulence and the ability to bind Congo red in Shigella flexneri 2a that is located on a 140-megadalton (230-kilobase) plasmid, was determined and analyzed. It was rich in A and T. The direction of transcription of virF was determined by using a chloramphenicol resistance cartridge. An open reading frame readable in this direction was found. Three proteins, 30, 27, and 21 kilodaltons, all corresponding to those predicted from the above sequence, were produced in minicells containing the virF locus. The three proteins were expressed only weakly in minicells with the 230-kilobase plasmid.

Entities:  

Mesh：

Substances：

Year:  1986        PMID： 3021627      PMCID： PMC260174          DOI： 10.1128/iai.54.2.395-402.1986

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  27 in total

Review 1.  Regulatory sequences involved in the promotion and termination of RNA transcription.

Authors:  M Rosenberg; D Court
Journal:  Annu Rev Genet       Date:  1979       Impact factor: 16.830

2.  Isolation of a small DNA fragment carrying the gene for a dihydrofolate reductase from a trimethoprim resistance factor.

Authors:  J W Zolg; U J Hänggi; H G Zachau
Journal:  Mol Gen Genet       Date:  1978-08-04

3.  Transformation in Escherichia coli: cryogenic preservation of competent cells.

Authors:  D A Morrison
Journal:  J Bacteriol       Date:  1977-10       Impact factor: 3.490

4.  IS50-mediated inverse transposition. Discrimination between the two ends of an IS element.

Authors:  C Sasakawa; D E Berg
Journal:  J Mol Biol       Date:  1982-08-05       Impact factor: 5.469

5.  Construction and characterization of the chloramphenicol-resistance gene cartridge: a new approach to the transcriptional mapping of extrachromosomal elements.

Authors:  T J Close; R L Rodriguez
Journal:  Gene       Date:  1982-12       Impact factor: 3.688

6.  Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.

Authors:  M J Casadaban; S N Cohen
Journal:  J Mol Biol       Date:  1980-04       Impact factor: 5.469

7.  Molecular alteration of the 140-megadalton plasmid associated with loss of virulence and Congo red binding activity in Shigella flexneri.

Authors:  C Sasakawa; K Kamata; T Sakai; S Y Murayama; S Makino; M Yoshikawa
Journal:  Infect Immun       Date:  1986-02       Impact factor: 3.441

8.  Plasmid replication functions. II. Cloning analysis of the repA replication region of antibiotic resistance plasmid R6-5.

Authors:  I Andrés; P M Slocombe; F Cabello; J K Timmis; R Lurz; H J Burkardt; K N Timmis
Journal:  Mol Gen Genet       Date:  1979-01-05

9.  Loss of pigmentation in Shigella flexneri 2a is correlated with loss of virulence and virulence-associated plasmid.

Authors:  A T Maurelli; B Blackmon; R Curtiss
Journal:  Infect Immun       Date:  1984-01       Impact factor: 3.441

10.  Involvement of a plasmid in the invasive ability of Shigella flexneri.

Authors:  P J Sansonetti; D J Kopecko; S B Formal
Journal:  Infect Immun       Date:  1982-03       Impact factor: 3.441
View more
  40 in total

1.  In vitro identification of Rns-regulated genes.

Authors:  George P Munson; Lisa G Holcomb; Heather L Alexander; June R Scott
Journal:  J Bacteriol       Date:  2002-02       Impact factor: 3.490

2.  virG, a plasmid-coded virulence gene of Shigella flexneri: identification of the virG protein and determination of the complete coding sequence.

Authors:  M C Lett; C Sasakawa; N Okada; T Sakai; S Makino; M Yamada; K Komatsu; M Yoshikawa
Journal:  J Bacteriol       Date:  1989-01       Impact factor: 3.490

Review 3.  Genetic basis of virulence in Shigella species.

Authors:  T L Hale
Journal:  Microbiol Rev       Date:  1991-06

4.  Thermoregulation of Shigella and Escherichia coli EIEC pathogenicity. A temperature-dependent structural transition of DNA modulates accessibility of virF promoter to transcriptional repressor H-NS.

Authors:  M Falconi; B Colonna; G Prosseda; G Micheli; C O Gualerzi
Journal:  EMBO J       Date:  1998-12-01       Impact factor: 11.598

Review 5.  Type III protein secretion systems in bacterial pathogens of animals and plants.

Authors:  C J Hueck
Journal:  Microbiol Mol Biol Rev       Date:  1998-06       Impact factor: 11.056

Review 6.  Arac/XylS family of transcriptional regulators.

Authors:  M T Gallegos; R Schleif; A Bairoch; K Hofmann; J L Ramos
Journal:  Microbiol Mol Biol Rev       Date:  1997-12       Impact factor: 11.056

Review 7.  Fungal virulence genes as targets for antifungal chemotherapy.

Authors:  J R Perfect
Journal:  Antimicrob Agents Chemother       Date:  1996-07       Impact factor: 5.191

8.  sar Genetic determinants necessary for transcription of RNAII and RNAIII in the agr locus of Staphylococcus aureus.

Authors:  A L Cheung; M G Bayer; J H Heinrichs
Journal:  J Bacteriol       Date:  1997-06       Impact factor: 3.490

9.  H-NS regulation of virulence gene expression in enteroinvasive Escherichia coli harboring the virulence plasmid integrated into the host chromosome.

Authors:  B Colonna; M Casalino; P A Fradiani; C Zagaglia; S Naitza; L Leoni; G Prosseda; A Coppo; P Ghelardini; M Nicoletti
Journal:  J Bacteriol       Date:  1995-08       Impact factor: 3.490

10.  Congo red-mediated regulation of levels of Shigella flexneri 2a membrane proteins.

Authors:  K Sankaran; V Ramachandran; Y V Subrahmanyam; S Rajarathnam; S Elango; R K Roy
Journal:  Infect Immun       Date:  1989-08       Impact factor: 3.441
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.