Ender Anılır1, Filiz Özen2, İbrahim Ali Özemir3, İbrahim Halil Yıldırım4, Çağrı Bilgiç5, Orhan Alimoğlu3. 1. Clinic of General Surgery, Amerikan Hospital, İstanbul, Turkey. 2. Department of Medical Genetics, İstanbul Medeniyet University School of Medicine, İstanbul, Turkey. 3. Department of General Surgery, İstanbul Medeniyet University School of Medicine, İstanbul, Turkey. 4. Department of Genetics, Dicle Üniversitesi School of Veterinary, Diyarbakır, Turkey. 5. Department of General Surgery, Koç University School of Medicine, İstanbul, Turkey.
Abstract
OBJECTIVES: There has been coverage of Toll-like receptor 4 and Toll-like receptor 2 gene polymorphisms in inflammatory episodes in a number of studies. In view of the inflammatory nature of acute pancreatitis, we aimed to determine the predictive value of mutations in Asp299Gly and Thr399Ile of the Toll-like receptor 4 gene, and the intron 2 microsatellite polymorphism of the Toll-like receptor 2 gene on the occurrence of acute biliary pancreatitis. MATERIAL AND METHODS: The study included 86 patients for the Toll-like receptor 4 Thr399Ile polymorphism, 100 patients for the Toll-like receptor 4 Asp299Gly polymorphism with acute biliary pancreatitis, and 101 healthy volunteers. At the same time, 93 patients and 92 healthy volunteers were included in the study to research the Toll-like receptor 2 intron 2 microsatellite polymorphism. Genotypes were determined using the restriction fragment length polymorphism analysis of PCR products and by an allele-specific PCR. RESULTS: The Toll-like receptor 4 Thr399Ile homozygotes mutant variants (p=0.005) and Toll-like receptor 2 MM genotype (p<0.001) were detected with a significantly higher frequency in patients with acute biliary pancreatitis than in the healthy blood donors. CONCLUSION: The Toll-like receptor 4 Asp299Gly and Thr399Ile polymorphisms and the Toll-like receptor 2 intron 2 microsatellite polymorphism are statistically associated with ABP.
OBJECTIVES: There has been coverage of Toll-like receptor 4 and Toll-like receptor 2 gene polymorphisms in inflammatory episodes in a number of studies. In view of the inflammatory nature of acute pancreatitis, we aimed to determine the predictive value of mutations in Asp299Gly and Thr399Ile of the Toll-like receptor 4 gene, and the intron 2 microsatellite polymorphism of the Toll-like receptor 2 gene on the occurrence of acute biliary pancreatitis. MATERIAL AND METHODS: The study included 86 patients for the Toll-like receptor 4 Thr399Ile polymorphism, 100 patients for the Toll-like receptor 4 Asp299Gly polymorphism with acute biliary pancreatitis, and 101 healthy volunteers. At the same time, 93 patients and 92 healthy volunteers were included in the study to research the Toll-like receptor 2 intron 2 microsatellite polymorphism. Genotypes were determined using the restriction fragment length polymorphism analysis of PCR products and by an allele-specific PCR. RESULTS: The Toll-like receptor 4 Thr399Ile homozygotes mutant variants (p=0.005) and Toll-like receptor 2 MM genotype (p<0.001) were detected with a significantly higher frequency in patients with acute biliary pancreatitis than in the healthy blood donors. CONCLUSION: The Toll-like receptor 4 Asp299Gly and Thr399Ile polymorphisms and the Toll-like receptor 2 intron 2 microsatellite polymorphism are statistically associated with ABP.
Authors: Annett Guenther; Ali Aghdassi; Venkata Muddana; Bettina Rau; Hans-Ulrich Schulz; Julia Mayerle; Matthias Kraft; David C Whitcomb; Markus M Lerch; Frank U Weiss Journal: Gut Date: 2010-06-29 Impact factor: 23.059
Authors: Armando Gabbrielli; Raffaele Pezzilli; Generoso Uomo; Alessandro Zerbi; Luca Frulloni; Paolo De Rai; Laura Castoldi; Guido Costamagna; Claudio Bassi; Valerio Di Carlo Journal: World J Gastrointest Endosc Date: 2010-09-16