Akihisa Kato1, Itaru Naitoh2, Katsuyuki Miyabe1, Kazuki Hayashi1, Michihiro Yoshida1, Yasuki Hori1, Makoto Natsume1, Naruomi Jinno1, Go Asano1, Hiroyuki Kato3, Toshiya Kuno3, Satoru Takahashi3, Hiromi Kataoka1. 1. Department of Gastroenterology and Metabolism, Graduate School of Medical Sciences, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, 467-8601, Japan. 2. Department of Gastroenterology and Metabolism, Graduate School of Medical Sciences, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, 467-8601, Japan. inaito@med.nagoya-cu.ac.jp. 3. Department of Experimental Pathology and Tumor Biology, Graduate School of Medical Sciences, Nagoya City University, Nagoya, Japan.
Abstract
BACKGROUND: The ability of fluorescence in situ hybridization (FISH) assays in endoscopic transpapillary bile duct biopsy specimens to predict the prognosis of cholangiocarcinoma (CCA) has not been elucidated. AIMS: We aimed to clarify the association between the results of UroVysion FISH assays and the prognosis of CCA. METHODS: We retrospectively reviewed 49 specimens obtained by transpapillary forceps biopsy from consecutive patients with CCA. The copy numbers of chromosomes 3, 7, and 17 were evaluated by FISH assay using UroVysion. We compared the overall survival (OS) of CCA patients with and without increased copy numbers of chromosomes 3, 7, and 17. Furthermore, we evaluated the association between OS and the clinicopathological parameters of CCA patients. RESULTS: The OS was significantly shorter in patients with than without an increased chromosome 7 copy number (log-rank p = 0.015; median OS 11.9 vs. 20.7 months). In the univariate analyses, age (p = 0.012), ECOG performance status (p = 0.046), tumor stage (p = 0.046), surgery (p = 0.006), and an increased chromosome 7 copy number (p = 0.017) were significantly associated with OS. The multivariate analysis revealed that an increased chromosome 7 copy number (hazard ratio, 2.46; 95% CI 1.15-5.27; p = 0.021) and advanced clinical stage (hazard ratio, 2.26; 95% CI 1.11-4.63; p = 0.025) were independently predictive of a poor OS. CONCLUSIONS: Detection by FISH assay of an increased chromosome 7 copy number in transpapillary forceps biopsy specimens is predictive of a poor prognosis in CCA patients.
BACKGROUND: The ability of fluorescence in situ hybridization (FISH) assays in endoscopic transpapillary bile duct biopsy specimens to predict the prognosis of cholangiocarcinoma (CCA) has not been elucidated. AIMS: We aimed to clarify the association between the results of UroVysion FISH assays and the prognosis of CCA. METHODS: We retrospectively reviewed 49 specimens obtained by transpapillary forceps biopsy from consecutive patients with CCA. The copy numbers of chromosomes 3, 7, and 17 were evaluated by FISH assay using UroVysion. We compared the overall survival (OS) of CCA patients with and without increased copy numbers of chromosomes 3, 7, and 17. Furthermore, we evaluated the association between OS and the clinicopathological parameters of CCA patients. RESULTS: The OS was significantly shorter in patients with than without an increased chromosome 7 copy number (log-rank p = 0.015; median OS 11.9 vs. 20.7 months). In the univariate analyses, age (p = 0.012), ECOG performance status (p = 0.046), tumor stage (p = 0.046), surgery (p = 0.006), and an increased chromosome 7 copy number (p = 0.017) were significantly associated with OS. The multivariate analysis revealed that an increased chromosome 7 copy number (hazard ratio, 2.46; 95% CI 1.15-5.27; p = 0.021) and advanced clinical stage (hazard ratio, 2.26; 95% CI 1.11-4.63; p = 0.025) were independently predictive of a poor OS. CONCLUSIONS: Detection by FISH assay of an increased chromosome 7 copy number in transpapillary forceps biopsy specimens is predictive of a poor prognosis in CCA patients.
Authors: Benjamin R Kipp; Linda M Stadheim; Shari A Halling; Nicole L Pochron; Scott Harmsen; David M Nagorney; Thomas J Sebo; Terry M Therneau; Gregory J Gores; Piet C de Groen; Todd H Baron; Michael J Levy; Kevin C Halling; Lewis R Roberts Journal: Am J Gastroenterol Date: 2004-09 Impact factor: 10.864
Authors: Arjun Nanda; Jason M Brown; Stephen H Berger; Melinda M Lewis; Emily G Barr Fritcher; Gregory J Gores; Steven A Keilin; Kevin E Woods; Qiang Cai; Field F Willingham Journal: Therap Adv Gastroenterol Date: 2015-03 Impact factor: 4.409
Authors: Tamas A Gonda; Michael P Glick; Amrita Sethi; John M Poneros; Walter Palmas; Shahzad Iqbal; Susana Gonzalez; Subhadra V Nandula; Jean C Emond; Robert S Brown; Vundavalli V Murty; Peter D Stevens Journal: Gastrointest Endosc Date: 2011-11-17 Impact factor: 9.427
Authors: Roongruedee Chaiteerakij; Emily G Barr Fritcher; Phonthep Angsuwatcharakon; Wiriyaporn Ridtitid; Supakarn Chaithongrat; Apinya Leerapun; Todd H Baron; Benjamin R Kipp; Michael R Henry; Kevin C Halling; Rungsun Rerknimitr; Lewis R Roberts Journal: Gastrointest Endosc Date: 2015-12-10 Impact factor: 9.427
Authors: Emily G Barr Fritcher; Jill L Caudill; Joshua E Blue; Kris Djuric; Lesley Feipel; Benard K Maritim; Ameera A Ragheb; Kevin C Halling; Michael R Henry; Amy C Clayton Journal: Am J Clin Pathol Date: 2011-09 Impact factor: 2.493
Authors: Emily G Barr Fritcher; Jesse S Voss; Shannon M Brankley; Michael B Campion; Sarah M Jenkins; Matthew E Keeney; Michael R Henry; Sarah M Kerr; Roongruedee Chaiteerakij; Ekaterina V Pestova; Amy C Clayton; Jun Zhang; Lewis R Roberts; Gregory J Gores; Kevin C Halling; Benjamin R Kipp Journal: Gastroenterology Date: 2015-08-29 Impact factor: 22.682