Literature DB >> 30203285

Engineered Cross-Linking to Study the Pore Architecture of the CRAC Channel.

Guolin Ma1, Lian He1, Ji Jing1, Peng Tan1, Yun Huang1, Yubin Zhou2,3.   

Abstract

ORAI1 constitutes the pore-forming subunit of the calcium release-activated calcium (CRAC) channel, a prototypical store-operated channel that is essential for the activation of cells of the immune system. Here we describe a convenient yet powerful cross-linking approach to examine the pore architecture of CRAC channels using ORAI1 proteins engineered to contain one or two cysteine residues. The generalizable cross-linking in situ approach can also be readily extended to study other integral membrane proteins expressed in various types of cells.

Entities:  

Keywords:  Calcium release-activated calcium channel; Calcium signaling; Cross-linking; Disulfide bond; Lymphocyte activation; Membrane protein; ORAI1; Store-operated calcium entry; Stromal interaction molecule; Structure

Mesh：

Substances：

Year:  2018        PMID： 30203285      PMCID： PMC8935632          DOI： 10.1007/978-1-4939-8704-7_13

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  61 in total

Review 1.  Store-operated CRAC channels: function in health and disease.

Authors:  Anant B Parekh
Journal:  Nat Rev Drug Discov       Date:  2010-04-16       Impact factor: 84.694

2.  STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane.

Authors:  Shenyuan L Zhang; Ying Yu; Jack Roos; J Ashot Kozak; Thomas J Deerinck; Mark H Ellisman; Kenneth A Stauderman; Michael D Cahalan
Journal:  Nature       Date:  2005-10-06       Impact factor: 49.962

3.  Large collective motions regulate the functional properties of glutamate transporter trimers.

Authors:  Jie Jiang; Indira H Shrivastava; Spencer D Watts; Ivet Bahar; Susan G Amara
Journal:  Proc Natl Acad Sci U S A       Date:  2011-08-29       Impact factor: 11.205

Review 4.  CRAC channelopathies.

Authors:  Stefan Feske
Journal:  Pflugers Arch       Date:  2010-01-29       Impact factor: 3.657

5.  STIM1 mutation associated with a syndrome of immunodeficiency and autoimmunity.

Authors:  Capucine Picard; Christie-Ann McCarl; Alexander Papolos; Sara Khalil; Kevin Lüthy; Claire Hivroz; Francoise LeDeist; Frédéric Rieux-Laucat; Gideon Rechavi; Anjana Rao; Alain Fischer; Stefan Feske
Journal:  N Engl J Med       Date:  2009-05-07       Impact factor: 91.245

Review 6.  Calcium signalling in lymphocyte activation and disease.

Authors:  Stefan Feske
Journal:  Nat Rev Immunol       Date:  2007-08-17       Impact factor: 53.106

7.  Determination of transmembrane protein structure by disulfide cross-linking: the Escherichia coli Tar receptor.

Authors:  A A Pakula; M I Simon
Journal:  Proc Natl Acad Sci U S A       Date:  1992-05-01       Impact factor: 11.205

Review 8.  Structure determination of membrane proteins by nuclear magnetic resonance spectroscopy.

Authors:  Stanley J Opella
Journal:  Annu Rev Anal Chem (Palo Alto Calif)       Date:  2013-04-01       Impact factor: 10.745

9.  STIM1 clusters and activates CRAC channels via direct binding of a cytosolic domain to Orai1.

Authors:  Chan Young Park; Paul J Hoover; Franklin M Mullins; Priti Bachhawat; Elizabeth D Covington; Stefan Raunser; Thomas Walz; K Christopher Garcia; Ricardo E Dolmetsch; Richard S Lewis
Journal:  Cell       Date:  2009-02-26       Impact factor: 41.582

Review 10.  ORAI1 and STIM1 deficiency in human and mice: roles of store-operated Ca2+ entry in the immune system and beyond.

Authors:  Stefan Feske
Journal:  Immunol Rev       Date:  2009-09       Impact factor: 12.988
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