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Literature DB >> 3020087

Viral isolation versus immune staining of infected cell cultures for the laboratory diagnosis of herpes simplex virus infections.

Abstract

The Difco Laboratories Cellmatics kit, an immune staining method for the detection of herpes simplex virus after amplification in culture, was found to be 94.6% (138 of 146) sensitive and 100% (302 of 302) specific. However, conventional culturing detected 10 (6.4%) additional viruses that were not herpes simplex. For a full-service diagnostic laboratory, viral isolation is important.

Entities: Disease

Mesh：

Substances：
Reagent Kits, Diagnostic

Year: 1986 PMID： 3020087 PMCID： PMC268946 DOI： 10.1128/jcm.24.3.487-489.1986

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

18 in total

1. Use of immunoperoxidase on brain tissue for the rapid diagnosis of herpes encephalitis.

Authors: D R Benjamin; C G Ray
Journal: Am J Clin Pathol Date: 1975-10 Impact factor: 2.493

2. Use of immunoperoxidase for rapid diagnosis of mucocutaneous herpes simplex virus infection.

Authors: D R Benjamin
Journal: J Clin Microbiol Date: 1977-12 Impact factor: 5.948

3. Detection of herpes simplex virus infection of female genitalia by the peroxidase-antiperoxidase method alone or in conjunction with the Papanicolaou stain.

Authors: N S Pearson; G Fleagle; J J Docherty
Journal: J Clin Microbiol Date: 1979-11 Impact factor: 5.948

4. Rapid herpes simplex virus detection in clinical samples submitted to a state virology laboratory.

Authors: D R Mayo; T Brennan; S H Egbertson; D F Moore
Journal: J Clin Microbiol Date: 1985-05 Impact factor: 5.948

5. Retrospective evaluation of the isolation and identification of herpes simplex virus with Cultureset and human fibroblasts.

Authors: L E Phillips; R A Magliolo; M L Stehlik; P A Whiteman; S Faro; T E Rogers
Journal: J Clin Microbiol Date: 1985-08 Impact factor: 5.948

6. Experiences in laboratory diagnosis of herpes simplex, varicella-zoster, and vaccinia virus infections in routine medical practice.

Authors: E C Herrmann
Journal: Mayo Clin Proc Date: 1967-11 Impact factor: 7.616

7. Evaluation of an enzyme-linked immunosorbent assay for the detection of herpes simplex virus antigen.

Authors: M A Morgan; T F Smith
Journal: J Clin Microbiol Date: 1984-06 Impact factor: 5.948

8. Comparison of Cultureset and primary rabbit kidney cell culture for the detection of herpes simplex virus.

Authors: S J Rubin; S Rogers
Journal: J Clin Microbiol Date: 1984-06 Impact factor: 5.948

9. Comparison of guinea pig embryo cells, rabbit kidney cells, and human embryonic lung fibroblast cell strains for isolation of herpes simplex virus.

Authors: M L Landry; D R Mayo; G D Hsiung
Journal: J Clin Microbiol Date: 1982-05 Impact factor: 5.948

10. Comparison of viral isolation, direct immunofluorescence, and indirect immunoperoxidase techniques for detection of genital herpes simplex virus infection.

Authors: R C Moseley; L Corey; D Benjamin; C Winter; M L Remington
Journal: J Clin Microbiol Date: 1981-05 Impact factor: 5.948

7 in total

1. Comparison of standard culture methods, a shell vial assay, and a DNA probe for the detection of herpes simplex virus.

Authors: L A Seal; P S Toyama; K M Fleet; K S Lerud; S R Heth; A J Moorman; J C Woods; R B Hill
Journal: J Clin Microbiol Date: 1991-03 Impact factor: 5.948

7. Impact of cell culture sensitivity and virus concentration on rapid detection of herpes simplex virus by cytopathic effects and immunoperoxidase staining.

Authors: L S Zhao; M L Landry; E S Balkovic; G D Hsiung
Journal: J Clin Microbiol Date: 1987-08 Impact factor: 5.948