Literature DB >> 30184715

A novel phase-shift-based amplitude detector for a high-speed atomic force microscope.

Atsushi Miyagi1, Simon Scheuring1.   

Abstract

In any atomic force microscope operated in amplitude modulation mode, aka "tapping mode" or "oscillating mode," the most crucial operation is the detection of the cantilever oscillation amplitude. Indeed, it is the change in the cantilever oscillation amplitude that drives the feedback loop, and thus, the accuracy and speed of amplitude detection are of utmost importance for improved atomic force microscopy operation. This becomes even more crucial for the operation of a high-speed atomic force microscope (HS-AFM), where feedback operation on a single or a low number of cantilever oscillation cycles between 500 kHz and 1000 kHz oscillation frequency is desired. So far, the amplitude detection was performed by Fourier analysis of each oscillation, resulting in a single output amplitude value at the end of each oscillation cycle, i.e., 360° phase delay. Here, we present a novel analog amplitude detection circuit with theoretic continuous amplitude detection at 90° phase delay. In factual operation, when exposed to an abrupt amplitude change, our novel amplitude detector circuit reacted with a phase delay of ∼138° compared with the phase delay of ∼682° achieved by the Fourier analysis method. Integrated to a HS-AFM, the novel amplitude detector should allow faster image acquisition with lower invasiveness due to the faster and more accurate detection of cantilever oscillation amplitude change.

Entities:  

Year:  2018        PMID: 30184715     DOI: 10.1063/1.5038095

Source DB:  PubMed          Journal:  Rev Sci Instrum        ISSN: 0034-6748            Impact factor:   1.523


  6 in total

1.  Visualizing the functional 3D shape and topography of long noncoding RNAs by single-particle atomic force microscopy and in-solution hydrodynamic techniques.

Authors:  Tina Uroda; Isabel Chillón; Paolo Annibale; Jean-Marie Teulon; Ombeline Pessey; Manikandan Karuppasamy; Jean-Luc Pellequer; Marco Marcia
Journal:  Nat Protoc       Date:  2020-05-25       Impact factor: 13.491

Review 2.  Advances in high-speed atomic force microscopy (HS-AFM) reveal dynamics of transmembrane channels and transporters.

Authors:  George R Heath; Simon Scheuring
Journal:  Curr Opin Struct Biol       Date:  2019-03-14       Impact factor: 6.809

3.  Localization atomic force microscopy.

Authors:  George R Heath; Ekaterina Kots; Janice L Robertson; Shifra Lansky; George Khelashvili; Harel Weinstein; Simon Scheuring
Journal:  Nature       Date:  2021-06-16       Impact factor: 49.962

4.  High-speed AFM height spectroscopy reveals µs-dynamics of unlabeled biomolecules.

Authors:  George R Heath; Simon Scheuring
Journal:  Nat Commun       Date:  2018-11-26       Impact factor: 14.919

5.  Chemically induced protein cage assembly with programmable opening and cargo release.

Authors:  Izabela Stupka; Yusuke Azuma; Artur P Biela; Motonori Imamura; Simon Scheuring; Elżbieta Pyza; Olga Woźnicka; Daniel P Maskell; Jonathan G Heddle
Journal:  Sci Adv       Date:  2022-01-05       Impact factor: 14.136

6.  Perforin-2 clockwise hand-over-hand pre-pore to pore transition mechanism.

Authors:  Fang Jiao; François Dehez; Tao Ni; Xiulian Yu; Jeremy S Dittman; Robert Gilbert; Christophe Chipot; Simon Scheuring
Journal:  Nat Commun       Date:  2022-08-26       Impact factor: 17.694

  6 in total

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