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Literature DB >> 3016719

Mechanism of DNA polymerase I: exonuclease/polymerase activity switch and DNA sequence dependence of pyrophosphorolysis and misincorporation reactions.

Abstract

Mechanistic features of several processes involved in the idling-turnover reaction catalyzed by the large (Klenow) fragment of Escherichia coli DNA polymerase I have been established. The exonuclease----polymerase activity switch involved in the excision/incorporation mode of idling-turnover occurs without an intervening dissociation of the enzyme from its DNA substrate. Comparative studies on the pyrophosphorolysis kinetics of related DNA substrates indicate a significant dependence of the reaction rate upon the DNA sequence within the duplex region upstream of the primer-template junction. Finally, a gel electrophoretic analysis of the products of the idling-turnover reaction has provided direct evidence for an alternative DNA sequence-dependent misincorporation/excision pathway.

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Year: 1986 PMID： 3016719 PMCID： PMC386376 DOI： 10.1073/pnas.83.16.5769

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

13 in total

1. Mechanism of primer-template-dependent conversion of dNTP leads to dNMP by T5 DNA polymerase.

Authors: S K Das; R K Fujimura
Journal: J Biol Chem Date: 1980-08-10 Impact factor: 5.157

2. A study of the mechanism of DNA polymerase I from Escherichia coli with diastereomeric phosphorothioate analogs of deoxyadenosine triphosphate.

Authors: P M Burgers; F Eckstein
Journal: J Biol Chem Date: 1979-08-10 Impact factor: 5.157

3. Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases.

Authors: D Brutlag; A Kornberg
Journal: J Biol Chem Date: 1972-01-10 Impact factor: 5.157

4. Enzymatic synthesis of deoxyribonucleic acid. 28. The pyrophosphate exchange and pyrophosphorolysis reactions of deoxyribonucleic acid polymerase.

Authors: M P Deutscher; A Kornberg
Journal: J Biol Chem Date: 1969-06-10 Impact factor: 5.157

5. Stereochemical course of the 3'----5'-exonuclease activity of DNA polymerase I.

Authors: A P Gupta; S J Benkovic
Journal: Biochemistry Date: 1984-11-20 Impact factor: 3.162

6. Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP.

Authors: D L Ollis; P Brick; R Hamlin; N G Xuong; T A Steitz
Journal: Nature Date: 1985 Feb 28-Mar 6 Impact factor: 49.962

7. Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli.

Authors: C M Joyce; N D Grindley
Journal: Proc Natl Acad Sci U S A Date: 1983-04 Impact factor: 11.205

8. Elementary steps in the DNA polymerase I reaction pathway.

Authors: F R Bryant; K A Johnson; S J Benkovic
Journal: Biochemistry Date: 1983-07-19 Impact factor: 3.162

9. Rate-limiting steps in the DNA polymerase I reaction pathway.

Authors: V Mizrahi; R N Henrie; J F Marlier; K A Johnson; S J Benkovic
Journal: Biochemistry Date: 1985-07-16 Impact factor: 3.162

10. Kinetics of base misinsertion by DNA polymerase I of Escherichia coli.

Authors: A R Fersht; J P Shi; W C Tsui
Journal: J Mol Biol Date: 1983-04-25 Impact factor: 5.469

15 in total

1. Effects of 2-chloroadenine substitution in DNA on restriction endonuclease cleavage reactions.

Authors: P Hentosh; J C McCastlain
Journal: Nucleic Acids Res Date: 1991-06-11 Impact factor: 16.971

2. Exploring the roles of nucleobase desolvation and shape complementarity during the misreplication of O(6)-methylguanine.

Authors: Delia Chavarria; Andrea Ramos-Serrano; Ichiro Hirao; Anthony J Berdis
Journal: J Mol Biol Date: 2011-07-23 Impact factor: 5.469

Mechanism of DNA polymerase I: exonuclease/polymerase activity switch and DNA sequence dependence of pyrophosphorolysis and misincorporation reactions.

1. Mechanism of primer-template-dependent conversion of dNTP leads to dNMP by T5 DNA polymerase.

2. A study of the mechanism of DNA polymerase I from Escherichia coli with diastereomeric phosphorothioate analogs of deoxyadenosine triphosphate.

3. Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases.

4. Enzymatic synthesis of deoxyribonucleic acid. 28. The pyrophosphate exchange and pyrophosphorolysis reactions of deoxyribonucleic acid polymerase.

5. Stereochemical course of the 3'----5'-exonuclease activity of DNA polymerase I.

6. Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP.

7. Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli.

8. Elementary steps in the DNA polymerase I reaction pathway.

9. Rate-limiting steps in the DNA polymerase I reaction pathway.

10. Kinetics of base misinsertion by DNA polymerase I of Escherichia coli.

1. Effects of 2-chloroadenine substitution in DNA on restriction endonuclease cleavage reactions.

2. Exploring the roles of nucleobase desolvation and shape complementarity during the misreplication of O(6)-methylguanine.

3. The carboxyl terminus of the bacteriophage T4 DNA polymerase is required for holoenzyme complex formation.

4. Factors affecting fidelity of DNA synthesis during PCR amplification of d(C-A)n.d(G-T)n microsatellite repeats.

5. Synthesis and application of derivatizable oligonucleotides.

6. Extrachromosomal recombination in mammalian cells as studied with single- and double-stranded DNA substrates.

7. Rapid assembly of the bacteriophage T4 core replication complex on a linear primer/template construct.

8. Effect of DNA sequence changes on UV mutability of a purine anticodon triplet of glyU cloned on M13 phage.

9. Mechanism and dynamics of translesion DNA synthesis catalyzed by the Escherichia coli Klenow fragment.

10. Development of a 'clickable' non-natural nucleotide to visualize the replication of non-instructional DNA lesions.