Literature DB >> 2038304

Stable transformation of the moss Physcomitrella patens.

D Schaefer1, J P Zryd, C D Knight, D J Cove.   

Abstract

We report the stable transformation of Physcomitrella patens to either G418 or hygromycin B resistance following polyethylene glycol-mediated direct DNA uptake by protoplasts. The method described in this paper was used successfully in independent experiments carried out in our two laboratories. Transformation was assessed by the following criteria: selection of antibiotic-resistant plants, mitotic and meiotic stability of phenotypes after removal of selective pressure and stable transmission of the character to the offspring; Southern hybridisation analysis of genomic DNA to show integration of the plasmid DNA; segregation of the resistance gene following crosses with antibiotic-sensitive strains; and finally Southern hybridisation analysis of both resistant and sensitive progeny. In addition to stable transformants, a heterogeneous class of unstable transformants was obtained.

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Year:  1991        PMID： 2038304     DOI： 10.1007/bf00260654

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  19 in total

1.  Genetic analysis by somatic hybridization of cytokinin overproducing developmental mutants of the moss, Physcomitrella patens.

Authors:  D R Featherstone; D J Cove; N W Ashton
Journal:  Mol Gen Genet       Date:  1990-07

Review 2.  Arabidopsis, a useful weed.

Authors:  E M Meyerowitz
Journal:  Cell       Date:  1989-01-27       Impact factor: 41.582

3.  Light requirements for regeneration of protoplasts of the moss Physcomitrella patens.

Authors:  G I Jenkins; D J Cove
Journal:  Planta       Date:  1983-02       Impact factor: 4.116

4.  Phototropism and polarotropism of primary chloronemata of the moss Physcomitrella patens: responses of the wild-type.

Authors:  G I Jenkins; D J Cove
Journal:  Planta       Date:  1983-08       Impact factor: 4.116

5.  Transcriptionally active genome regions are preferred targets for retrovirus integration.

Authors:  U Scherdin; K Rhodes; M Breindl
Journal:  J Virol       Date:  1990-02       Impact factor: 5.103

6.  Analysis of gametophytic development in the moss, Physcomitrella patens, using auxin and cytokinin resistant mutants.

Authors:  N W Ashton; N H Grimsley; D J Cove
Journal:  Planta       Date:  1979-01       Impact factor: 4.116

7.  A genomic DNA segment from Petunia hybrida leads to increased transformation frequencies and simple integration patterns.

Authors:  P Meyer; S Kartzke; I Niedenhof; I Heidmann; K Bussmann; H Saedler
Journal:  Proc Natl Acad Sci U S A       Date:  1988-11       Impact factor: 11.205

8.  ADP-ribosyltransferase activity in cultured hepatocytes. Interactions with DNA repair.

Authors:  F R Althaus; S D Lawrence; G L Sattler; H C Pitot
Journal:  J Biol Chem       Date:  1982-05-25       Impact factor: 5.157

9.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1983-07-01       Impact factor: 3.365

10.  Agarose plating and a bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant species.

Authors:  R D Shillito; J Paszkowski; I Potrykus
Journal:  Plant Cell Rep       Date:  1983-10       Impact factor: 4.570
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  84 in total

Review 1.  A tool for understanding homologous recombination in plants.

Authors:  A Hohe; R Reski
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2.  Programmed Changes in Form during Moss Development.

Authors:  K. S. Schumaker; M. A. Dietrich
Journal:  Plant Cell       Date:  1997-07       Impact factor: 11.277

3.  The Moss Physcomitrella patens, a Model System with Potential for the Study of Plant Reproduction.

Authors:  D. J. Cove; C. D. Knight
Journal:  Plant Cell       Date:  1993-10       Impact factor: 11.277

Review 4.  From axenic spore germination to molecular farming. One century of bryophyte in vitro culture.

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5.  Actin-related protein2/3 complex component ARPC1 is required for proper cell morphogenesis and polarized cell growth in Physcomitrella patens.

Authors:  Phillip A Harries; Aihong Pan; Ralph S Quatrano
Journal:  Plant Cell       Date:  2005-07-08       Impact factor: 11.277

6.  Genetic analysis of a mutant class of Physcomitrella patens in which the polarity of gravitropism is reversed.

Authors:  C D Knight; T S Futers; D J Cove
Journal:  Mol Gen Genet       Date:  1991-11

7.  An LL-diaminopimelate aminotransferase defines a novel variant of the lysine biosynthesis pathway in plants.

Authors:  André O Hudson; Bijay K Singh; Thomas Leustek; Charles Gilvarg
Journal:  Plant Physiol       Date:  2005-12-16       Impact factor: 8.340

8.  Multiple conserved 5' elements are required for high-level pollen expression of the Arabidopsis reproductive actin ACT1.

Authors:  Angela Vitale; Ray J Wu; Zaiquan Cheng; Richard B Meagher
Journal:  Plant Mol Biol       Date:  2003-08       Impact factor: 4.076

9.  Molecular Responses to Abscisic Acid and Stress Are Conserved between Moss and Cereals.

Authors:  C. D. Knight; A. Sehgal; K. Atwal; J. C. Wallace; D. J. Cove; D. Coates; R. S. Quatrano; S. Bahadur; P. G. Stockley; A. C. Cuming
Journal:  Plant Cell       Date:  1995-05       Impact factor: 11.277

10.  RNA interference in the moss Physcomitrella patens.

Authors:  Magdalena Bezanilla; Aihong Pan; Ralph S Quatrano
Journal:  Plant Physiol       Date:  2003-10       Impact factor: 8.340
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