Literature DB >> 3016642

Floral tissue of Petunia hybrida (V30) expresses only one member of the chalcone synthase multigene family.

R E Koes, C E Spelt, H J Reif, P J van den Elzen, E Veltkamp, J N Mol.   

Abstract

Twenty independent, petal-specific chalcone synthase (CHS) cDNA clones have been isolated from Petunia hybrida variety Violet 30 (V30). Sequence analysis shows that the largest of these clones contains the entire coding sequence. Using this clone in Southern blot analysis reveals the presence of multiple CHS gene copies in the genome of Petunia hybrida V30. Hybridization and sequence analysis of the CHS cDNA clones shows that they are all copied from a single mRNA species. This indicates the presence of only one transcriptionally active CHS gene in petals. Finally we report the identification, cloning and partial characterization of this gene.

Entities:  

Mesh:

Substances:

Year:  1986        PMID: 3016642      PMCID: PMC311537          DOI: 10.1093/nar/14.13.5229

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN: 0305-1048            Impact factor:   16.971


  25 in total

1.  In vitro packaging of lambda and cosmid DNA.

Authors:  B Hohn
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

2.  EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system.

Authors:  P Leder; D Tiemeier; L Enquist
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

3.  A simple and very efficient method for generating cDNA libraries.

Authors:  U Gubler; B J Hoffman
Journal:  Gene       Date:  1983-11       Impact factor: 3.688

4.  Lambda replacement vectors carrying polylinker sequences.

Authors:  A M Frischauf; H Lehrach; A Poustka; N Murray
Journal:  J Mol Biol       Date:  1983-11-15       Impact factor: 5.469

5.  "Nonrandom" DNA sequence analysis in bacteriophage M13 by the dideoxy chain-termination method.

Authors:  M Poncz; D Solowiejczyk; M Ballantine; E Schwartz; S Surrey
Journal:  Proc Natl Acad Sci U S A       Date:  1982-07       Impact factor: 11.205

6.  Coordinated regulation of 4-coumarate:CoA ligase and phenylalanine ammonia-lyase mRNAs in cultured plant cells.

Authors:  H Ragg; D N Kuhn; K Hahlbrock
Journal:  J Biol Chem       Date:  1981-10-10       Impact factor: 5.157

7.  Concomitant induction of phenylalanine ammonia-lyase and flavanone synthase mRNAs in irradiated plant cells.

Authors:  J Schröder; F Kreuzaler; E Schäfer; K Hahlbrock
Journal:  J Biol Chem       Date:  1979-01-10       Impact factor: 5.157

8.  Light-inducible and tissue-specific expression of a chimaeric gene under control of the 5'-flanking sequence of a pea chlorophyll a/b-binding protein gene.

Authors:  J Simpson; M P Timko; A R Cashmore; J Schell; M V Montagu; L Herrera-Estrella
Journal:  EMBO J       Date:  1985-11       Impact factor: 11.598

9.  Photoregulated expression of a pea rbcS gene in leaves of transgenic plants.

Authors:  F Nagy; G Morelli; R T Fraley; S G Rogers; N H Chua
Journal:  EMBO J       Date:  1985-12-01       Impact factor: 11.598

10.  Coding and 3' non-coding nucleotide sequence of chalcone synthase mRNA and assignment of amino acid sequence of the enzyme.

Authors:  U Reimold; M Kröger; F Kreuzaler; K Hahlbrock
Journal:  EMBO J       Date:  1983       Impact factor: 11.598

View more
  35 in total

1.  Posttranscriptional gene silencing of gn1 in tobacco triggers accumulation of truncated gn1-derived RNA species.

Authors:  K Litière; G J van Eldik; J J Jacobs; M Van Montagu; M Cornelissen
Journal:  RNA       Date:  1999-10       Impact factor: 4.942

2.  Leaf-Mediated Light Responses in Petunia Flowers.

Authors:  S. Moscovici; D. Moalem-Beno; D. Weiss
Journal:  Plant Physiol       Date:  1996-04       Impact factor: 8.340

3.  Codon usage in plant genes.

Authors:  E E Murray; J Lotzer; M Eberle
Journal:  Nucleic Acids Res       Date:  1989-01-25       Impact factor: 16.971

4.  Expression of chalcone synthase and chalcone isomerase proteins in Arabidopsis seedlings.

Authors:  C C Cain; D E Saslowsky; R A Walker; B W Shirley
Journal:  Plant Mol Biol       Date:  1997-10       Impact factor: 4.076

5.  Regulation of chalcone flavanone isomerase (CHI) gene expression inPetunia hybrida: the use of alternative promoters in corolla, anthers and pollen.

Authors:  A J van Tunen; S A Hartman; L A Mur; J N Mol
Journal:  Plant Mol Biol       Date:  1989-05       Impact factor: 4.076

6.  Expression of chalcone synthase, dihydroflavonol reductase, and flavanone-3-hydroxylase in mutants of barley deficient in anthocyanin and proanthocyanidin biosynthesis.

Authors:  M Meldgaard
Journal:  Theor Appl Genet       Date:  1992-04       Impact factor: 5.699

7.  Expression of small heat-shock proteins at low temperatures. A possible role in protecting against chilling injuries.

Authors:  A Sabehat; S Lurie; D Weiss
Journal:  Plant Physiol       Date:  1998-06       Impact factor: 8.340

8.  Sugar-Dependent Gibberellin-Induced Chalcone Synthase Gene Expression in Petunia Corollas.

Authors:  D. Moalem-Beno; G. Tamari; Y. Leitner-Dagan; A. Borochov; D. Weiss
Journal:  Plant Physiol       Date:  1997-02       Impact factor: 8.340

9.  Identification of Endogenous Gibberellins in Petunia Flowers (Induction of Anthocyanin Biosynthetic Gene Expression and the Antagonistic Effect of Abscisic Acid).

Authors:  D. Weiss; A. Van Der Luit; E. Knegt; E. Vermeer; JNM. Mol; J. M. Kooter
Journal:  Plant Physiol       Date:  1995-03       Impact factor: 8.340

10.  Differential expression of two MADS box genes in wild-type and mutant petunia flowers.

Authors:  G C Angenent; M Busscher; J Franken; J N Mol; A J van Tunen
Journal:  Plant Cell       Date:  1992-08       Impact factor: 11.277

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.