C Montalva1,2, J J Silva1, L F N Rocha1,3, C Luz1, R A Humber1,4. 1. Laboratório de Patologia de Invertebrados, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Goiás, Brazil. 2. Facultad de Ciencias, Instituto de Bioquímica y Microbiología, Universidad Austral de Chile (Uach), Valdivia, Chile. 3. Instituto Federal de Educação, Ciência e Tecnologia de Goiás, Aparecida de Goiânia, Goiás, Brazil. 4. USDA-ARS Emerging Pests and Pathogens Research Unit, Robert W. Holley Center for Agriculture and Health, Ithaca, NY, USA.
Abstract
AIMS: To survey and to characterize entomopathogenic fungi as natural enemies of mosquitoes in Central Brazil. METHODS AND RESULTS: Tolypocladium cylindrosporum (Hypocreales, Ophiocordycipitaceae) was isolated for the first time in South America by using Aedes aegypti (Diptera, Culicidae) as sentinel larvae in a stagnant mosquito breeding site in a secondary tropical forest. Two isolates were morphologically and molecularly identified, and their activity tested against A. aegypti eggs, larvae and adults. CONCLUSIONS: Findings emphasize the importance of T. cylindrosporum as a natural fungal antagonist of mosquitoes. SIGNIFICANCE AND IMPACT OF THE STUDY: Both isolates affected larvae and adults but were less effective against eggs; both have potential for development as a mycoinsecticide especially against larvae of A. aegypti the main vector of dengue, chikungunya, Zika and urban yellow fever.
AIMS: To survey and to characterize entomopathogenic fungi as natural enemies of mosquitoes in Central Brazil. METHODS AND RESULTS:Tolypocladium cylindrosporum (Hypocreales, Ophiocordycipitaceae) was isolated for the first time in South America by using Aedes aegypti (Diptera, Culicidae) as sentinel larvae in a stagnant mosquito breeding site in a secondary tropical forest. Two isolates were morphologically and molecularly identified, and their activity tested against A. aegypti eggs, larvae and adults. CONCLUSIONS: Findings emphasize the importance of T. cylindrosporum as a natural fungal antagonist of mosquitoes. SIGNIFICANCE AND IMPACT OF THE STUDY: Both isolates affected larvae and adults but were less effective against eggs; both have potential for development as a mycoinsecticide especially against larvae of A. aegypti the main vector of dengue, chikungunya, Zika and urban yellow fever.
Authors: Manuel E Rueda Páramo; Karine R Dos Santos; Marcos D G Filgueiras; Éverton K K Fernandes; Cristian Montalva; Richard A Humber; Christian Luz Journal: Neotrop Entomol Date: 2022-05-16 Impact factor: 1.434
Authors: Juscelino Rodrigues; Luiz F N Rocha; Juan M Martinez; Cristian Montalva; Richard A Humber; Christian Luz Journal: Parasitol Res Date: 2022-08-22 Impact factor: 2.383