Literature DB >> 301453

Lack of subclass selection during density purification of human lymphocytes.

P Häyry, T H Tötterman, A Ranki.   

Abstract

The percentage of acid alpha-naphthyl acetate esterase (ANAE) marker-carrying lymphocytes (T-lymphocytes) was determined directly from the blood, after density purification of lymphocytes over Ficoll-Isopaque and removal of monocytes by iron powder plus magnet. The effect of Ficoll-Isopaque centrifugation was insignificant; neither did the iron powder plus magnet procedure increase the relative proportion of the T cells in these preparations. We therefore conclude that in optimal conditions there is no subclass-specific enrichment of T or B lymphocytes during the purification procedure.

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Year:  1977        PMID： 301453      PMCID： PMC1540756     

Source DB:  PubMed          Journal:  Clin Exp Immunol        ISSN： 0009-9104            Impact factor:   4.330


  10 in total

1.  Enumeration of absolute numbers of T and B lymphocytes in human blood.

Authors:  G Brown; M F Greaves
Journal:  Scand J Immunol       Date:  1974       Impact factor: 3.487

2.  Evidence for individual human peripheral blood lymphocytes bearing both B and T cell markers.

Authors:  H B Dickler; N F Adkinson; W D Terry
Journal:  Nature       Date:  1974-01-25       Impact factor: 49.962

3.  Cell surface markers for human T and B lymphocytes.

Authors:  G Brown; M F Greaves
Journal:  Eur J Immunol       Date:  1974-04       Impact factor: 5.532

4.  A subpopulation of T cells bearing Fc receptors.

Authors:  A Basten; J F Miller; N L Warner; R Abraham; E Chia; J Gamble
Journal:  J Immunol       Date:  1975-10       Impact factor: 5.422

5.  Identification of mouse T and B lymphocytes from cytocentrifuged cell smears.

Authors:  A Ranki; T H Tötterman; P Häyry
Journal:  Clin Exp Immunol       Date:  1976-12       Impact factor: 4.330

6.  Identification of resting human T and B lymphocytes by acid alpha-naphthyl acetate esterase staining combined with rosette formation with Staphylococcus aureus strain Cowan 1.

Authors:  A Ranki; T H Tötterman; P Häyry
Journal:  Scand J Immunol       Date:  1976       Impact factor: 3.487

7.  Immunoglobulins as cell receptors.

Authors:  B Pernis; L Forni; L Amante
Journal:  Ann N Y Acad Sci       Date:  1971-12-31       Impact factor: 5.691

8.  Receptors for complement and immunoglobulin on human leukemic cells and human lymphoblastoid cell lines.

Authors:  E M Shevach; R Herberman; M M Frank; I Green
Journal:  J Clin Invest       Date:  1972-08       Impact factor: 14.808

9.  Interaction of aggregated -globulin with B lymphocytes.

Authors:  H B Dickler; H G Kunkel
Journal:  J Exp Med       Date:  1972-07-01       Impact factor: 14.307

10.  Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells.

Authors:  M Jondal; G Holm; H Wigzell
Journal:  J Exp Med       Date:  1972-08-01       Impact factor: 14.307
  10 in total
  4 in total

1.  In vitro T- and B-cell reactivity in cartilage hair hypoplasia.

Authors:  A Ranki; J Perheentupa; L C Andersson; P Häyry
Journal:  Clin Exp Immunol       Date:  1978-05       Impact factor: 4.330

2.  Characterization of the human peripheral effector cells mediating antibody dependent cellular cytotoxicity against allogenic cells.

Authors:  M Donner; C Raffoux; F Streiff
Journal:  Clin Exp Immunol       Date:  1979-12       Impact factor: 4.330

3.  Blood and thyroid-infiltrating lymphocyte subclasses in juvenile autoimmune thyroiditis.

Authors:  T H Tötterman; J Mäenpää; A Gordin; T Mäkinen; E Taskinens; L C Andersson; P Häyry
Journal:  Clin Exp Immunol       Date:  1977-11       Impact factor: 4.330

4.  A ganglioside antigen on the rat pancreatic B cell surface identified by monoclonal antibody R2D6.

Authors:  R Alejandro; F L Shienvold; S A Hajek; M Pierce; R Paul; D H Mintz
Journal:  J Clin Invest       Date:  1984-07       Impact factor: 14.808
  4 in total

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