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Literature DB >> 3014501

Replication of a plasmid bearing a human Alu-family repeat in monkey COS-7 cells.

Abstract

Monkey COS-7 cells were transformed with BLUR8 DNA, a pBR322 plasmid containing a human Alu-family sequence at the BamHI site. Within 24 hr of transformation 2-5% of the BLUR8 molecules recovered resisted cleavage by Dpn I, indicating they had replicated. Electron microscopy revealed appropriately sized circular molecules with replication bubbles whose centers were mapped to the Alu insert. A 16-base-pair deletion within the Alu sequence prevented replication. The results indicate that certain Alu sequences can serve as origins of replication in COS-7 cells.

Entities: Species

Mesh：

Substances：
Antigens, Viral, Tumor

Year: 1986 PMID： 3014501 PMCID： PMC323801 DOI： 10.1073/pnas.83.13.4660

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

30 in total

1. DNA infectivity and the induction of host DNA synthesis with temperature-sensitive mutants of simian virus 40.

Authors: J Y Chou; R G Martin
Journal: J Virol Date: 1975-01 Impact factor: 5.103

2. The genome of simian virus 40.

Authors: V B Reddy; B Thimmappaya; R Dhar; K N Subramanian; B S Zain; J Pan; P K Ghosh; M L Celma; S M Weissman
Journal: Science Date: 1978-05-05 Impact factor: 47.728

3. Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation.

Authors: S Lacks; B Greenberg
Journal: J Mol Biol Date: 1977-07 Impact factor: 5.469

4. Monomers through trimers of large tumor antigen bind in region I and monomers through tetramers bind in region II of simian virus 40 origin of replication DNA as stable structures in solution.

Authors: I A Mastrangelo; P V Hough; V G Wilson; J S Wall; J F Hainfeld; P Tegtmeyer
Journal: Proc Natl Acad Sci U S A Date: 1985-06 Impact factor: 11.205

5. Properties of some monkey DNA sequences obtained by a procedure that enriches for DNA replication origins.

Authors: M Zannis-Hadjopoulos; G Kaufmann; S S Wang; R L Lechner; E Karawya; J Hesse; R G Martin
Journal: Mol Cell Biol Date: 1985-07 Impact factor: 4.272

6. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.

Authors: G M Wahl; M Stern; G R Stark
Journal: Proc Natl Acad Sci U S A Date: 1979-08 Impact factor: 11.205

7. Selective extraction of polyoma DNA from infected mouse cell cultures.

Authors: B Hirt
Journal: J Mol Biol Date: 1967-06-14 Impact factor: 5.469

8. A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors: F L Graham; A J van der Eb
Journal: Virology Date: 1973-04 Impact factor: 3.616

9. A ubiquitous family of repeated DNA sequences in the human genome.

Authors: C M Houck; F P Rinehart; C W Schmid
Journal: J Mol Biol Date: 1979-08-15 Impact factor: 5.469

10. Sequence-dependent DNA replication in preimplantation mouse embryos.

Authors: D O Wirak; L E Chalifour; P M Wassarman; W J Muller; J A Hassell; M L DePamphilis
Journal: Mol Cell Biol Date: 1985-11 Impact factor: 4.272

23 in total

1. Organization of specific DNA sequence elements in the region of the replication origin and matrix attachment site in the chicken alpha-globin gene domain.

Authors: W A Krajewski; S V Razin
Journal: Mol Gen Genet Date: 1992-11

9. The Chinese hamster dihydrofolate reductase replication origin beta is active at multiple ectopic chromosomal locations and requires specific DNA sequence elements for activity.

Authors: A L Altman; E Fanning
Journal: Mol Cell Biol Date: 2001-02 Impact factor: 4.272

10. Mapping initiation sites of DNA replication in vivo using polymerase chain reaction amplification of nascent strand segments.

Authors: L Vassilev; E M Johnson
Journal: Nucleic Acids Res Date: 1989-10-11 Impact factor: 16.971

Replication of a plasmid bearing a human Alu-family repeat in monkey COS-7 cells.

1. DNA infectivity and the induction of host DNA synthesis with temperature-sensitive mutants of simian virus 40.

2. The genome of simian virus 40.

3. Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation.

4. Monomers through trimers of large tumor antigen bind in region I and monomers through tetramers bind in region II of simian virus 40 origin of replication DNA as stable structures in solution.

5. Properties of some monkey DNA sequences obtained by a procedure that enriches for DNA replication origins.

6. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.

7. Selective extraction of polyoma DNA from infected mouse cell cultures.

8. A new technique for the assay of infectivity of human adenovirus 5 DNA.

9. A ubiquitous family of repeated DNA sequences in the human genome.

10. Sequence-dependent DNA replication in preimplantation mouse embryos.

1. Organization of specific DNA sequence elements in the region of the replication origin and matrix attachment site in the chicken alpha-globin gene domain.

2. Transfer RNA-like structure of the human Alu family: implications of its generation mechanism and possible functions.

3. Free left arms as precursor molecules in the evolution of Alu sequences.

Review 4. Emergence of master sequences in families of retroposons derived from 7sl RNA.

5. Autonomous replication of a DNA fragment containing the chromosomal replication origin of the human c-myc gene.

6. Evaluation of autonomous plasmid replication in transfected mammalian cells.

7. Asbestos fibers mediate transformation of monkey cells by exogenous plasmid DNA.

8. Alu-family repeat binding protein from HeLa cells which interacts with regulatory region of SV40 virus genome.

9. The Chinese hamster dihydrofolate reductase replication origin beta is active at multiple ectopic chromosomal locations and requires specific DNA sequence elements for activity.

10. Mapping initiation sites of DNA replication in vivo using polymerase chain reaction amplification of nascent strand segments.