Juan Zhao1, Zhuoya Wan2, Chuchu Zhou1, Qin Yang1, Jianxia Dong3, Xu Song4, Tao Gong1. 1. Key Laboratory of Drug Targeting and Drug Delivery Systems, Ministry of Education, Sichuan University, No. 17, Block 3 Southern Renmin Road, Chengdu, 610041, China. 2. School of Pharmacy, University of Pittsburgh, Pittsburgh, Pennsylvania, 15217, USA. 3. Department of Clinical Pharmacy, West China Hospital of Sichuan University, Chengdu, 610041, Sichuan Province, People's Republic of China. 4. Key Laboratory of Drug Targeting and Drug Delivery Systems, Ministry of Education, Sichuan University, No. 17, Block 3 Southern Renmin Road, Chengdu, 610041, China. xusong2016@scu.edu.cn.
Abstract
PURPOSE: The aim of this study was to design hyaluronic acid (HA) layer-by-layer (LbL) nanoparticles, which carried paclitaxel (PTX) and Indocyanine green (ICG) to both tumor cells and tumor associated cells to achieve synergistic chemo-photothermal therapeutic effect. METHODS: The LbL-engineered nanoparticles (PDIH) were prepared by dopamine self-polymerization on PTX nanocrystal to form thin, surface-adherent polydopamine (PDA) films, which subsequently absorbed ICG and HA. The tumor cell and tumor associated cell targeting and antitumor efficacy of PDIH were investigated both in vitro an in vivo using 4 T1 murine mammary cancer cell lines and mice bearing orthotopic 4 T1 breast tumor. RESULTS: PDIH presented a long-rod shape in TEM and showed enhanced photothermal effect and cytotoxicity upon NIR laser irradiation both in vitro and in vivo. PDIH also displayed high target ability to CD44 overexpressed tumor cells and tumor associated cells mediated by HA. In vivo antitumor study indicated that PDIH therapeutic strategy could achieve remarkable antitumor efficacy. CONCLUSION: PDIH showed excellent tumor-targeting property and chemo-photothermal therapeutic efficacy.
PURPOSE: The aim of this study was to design hyaluronic acid (HA) layer-by-layer (LbL) nanoparticles, which carried paclitaxel (PTX) and Indocyanine green (ICG) to both tumor cells and tumor associated cells to achieve synergistic chemo-photothermal therapeutic effect. METHODS: The LbL-engineered nanoparticles (PDIH) were prepared by dopamine self-polymerization on PTX nanocrystal to form thin, surface-adherent polydopamine (PDA) films, which subsequently absorbed ICG and HA. The tumor cell and tumor associated cell targeting and antitumor efficacy of PDIH were investigated both in vitro an in vivo using 4 T1 murine mammary cancer cell lines and mice bearing orthotopic 4 T1 breast tumor. RESULTS: PDIH presented a long-rod shape in TEM and showed enhanced photothermal effect and cytotoxicity upon NIR laser irradiation both in vitro and in vivo. PDIH also displayed high target ability to CD44 overexpressed tumor cells and tumor associated cells mediated by HA. In vivo antitumor study indicated that PDIH therapeutic strategy could achieve remarkable antitumor efficacy. CONCLUSION: PDIH showed excellent tumor-targeting property and chemo-photothermal therapeutic efficacy.
Authors: Yuan Ma; Sifan Yu; Shuaijian Ni; Baoxian Zhang; Angela Chun Fai Kung; Jin Gao; Aiping Lu; Ge Zhang Journal: Front Cell Dev Biol Date: 2021-03-29