Literature DB >> 30134012

Using MbtH-Like Proteins to Alter the Substrate Profile of a Nonribosomal Peptide Adenylation Enzyme.

Shogo Mori1, Keith D Green1, Ryan Choi2,3, Garry W Buchko3,4,5, Michael G Fried6, Sylvie Garneau-Tsodikova1.   

Abstract

MbtH-like proteins (MLPs) are required for soluble expression and/or optimal activity of some adenylation (A) domains of nonribosomal peptide synthetases. Because A domains can interact with noncognate MLP partners, how the function of an A domain, TioK, involved in the biosynthesis of the bisintercalator thiocoraline, is altered by noncognate MLPs has been investigated. Measuring TioK activity with 12 different MLPs from a variety of bacterial species by using a radiometric assay suggested that the A domain substrate promiscuity could be altered by foreign MLPs. Kinetic studies and bioinformatics analysis expanded the complexity of MLP functions and interactions.
© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  bioinformatics; biosynthesis; enzymes; kinetics; protein-protein interactions

Mesh：

Substances：

Year:  2018        PMID： 30134012      PMCID： PMC6439349          DOI： 10.1002/cbic.201800240

Source DB:  PubMed          Journal:  Chembiochem        ISSN： 1439-4227            Impact factor:   3.164


  39 in total

1.  Effects of deletions of mbtH-like genes on clorobiocin biosynthesis in Streptomyces coelicolor.

Authors:  Manuel Wolpert; Bertolt Gust; Bernd Kammerer; Lutz Heide
Journal:  Microbiology       Date:  2007-05       Impact factor: 2.777

Review 2.  Insights into the chemical logic and enzymatic machinery of NRPS assembly lines.

Authors:  Christopher T Walsh
Journal:  Nat Prod Rep       Date:  2016-02       Impact factor: 13.423

3.  Analyses of MbtB, MbtE, and MbtF suggest revisions to the mycobactin biosynthesis pathway in Mycobacterium tuberculosis.

Authors:  Matthew D McMahon; Jason S Rush; Michael G Thomas
Journal:  J Bacteriol       Date:  2012-03-23       Impact factor: 3.490

4.  MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets.

Authors:  Sudhir Kumar; Glen Stecher; Koichiro Tamura
Journal:  Mol Biol Evol       Date:  2016-03-22       Impact factor: 16.240

5.  The neighbor-joining method: a new method for reconstructing phylogenetic trees.

Authors:  N Saitou; M Nei
Journal:  Mol Biol Evol       Date:  1987-07       Impact factor: 16.240

6.  Structural basis for backbone N-methylation by an interrupted adenylation domain.

Authors:  Shogo Mori; Allan H Pang; Taylor A Lundy; Atefeh Garzan; Oleg V Tsodikov; Sylvie Garneau-Tsodikova
Journal:  Nat Chem Biol       Date:  2018-03-19       Impact factor: 15.040

7.  Directed evolution of the nonribosomal peptide synthetase AdmK generates new andrimid derivatives in vivo.

Authors:  Bradley S Evans; Yunqiu Chen; William W Metcalf; Huimin Zhao; Neil L Kelleher
Journal:  Chem Biol       Date:  2011-05-27

8.  Enzymatic tailoring of ornithine in the biosynthesis of the Rhizobium cyclic trihydroxamate siderophore vicibactin.

Authors:  John R Heemstra; Christopher T Walsh; Elizabeth S Sattely
Journal:  J Am Chem Soc       Date:  2009-10-28       Impact factor: 15.419

9.  Structural basis of the interaction of MbtH-like proteins, putative regulators of nonribosomal peptide biosynthesis, with adenylating enzymes.

Authors:  Dominik A Herbst; Björn Boll; Georg Zocher; Thilo Stehle; Lutz Heide
Journal:  J Biol Chem       Date:  2012-11-28       Impact factor: 5.157

10.  MbtH-like protein-mediated cross-talk between non-ribosomal peptide antibiotic and siderophore biosynthetic pathways in Streptomyces coelicolor M145.

Authors:  Sylvie Lautru; Daniel Oves-Costales; Jean-Luc Pernodet; Gregory L Challis
Journal:  Microbiology (Reading)       Date:  2007-05       Impact factor: 2.777
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  5 in total

Review 1.  Leveraging synthetic biology for producing bioactive polyketides and non-ribosomal peptides in bacterial heterologous hosts.

Authors:  Taylor B Cook; Brian F Pfleger
Journal:  Medchemcomm       Date:  2019-04-25       Impact factor: 3.597

2.  Lessons learned in engineering interrupted adenylation domains when attempting to create trifunctional enzymes from three independent monofunctional ones.

Authors:  Taylor A Lundy; Shogo Mori; Sylvie Garneau-Tsodikova
Journal:  RSC Adv       Date:  2020-09-15       Impact factor: 4.036

3.  TxtH is a key component of the thaxtomin biosynthetic machinery in the potato common scab pathogen Streptomyces scabies.

Authors:  Yuting Li; Jingyu Liu; Damilola Adekunle; Luke Bown; Kapil Tahlan; Dawn R D Bignell
Journal:  Mol Plant Pathol       Date:  2019-07-07       Impact factor: 5.663

4.  The structural basis of N-acyl-α-amino-β-lactone formation catalyzed by a nonribosomal peptide synthetase.

Authors:  Dale F Kreitler; Erin M Gemmell; Jason E Schaffer; Timothy A Wencewicz; Andrew M Gulick
Journal:  Nat Commun       Date:  2019-07-31       Impact factor: 14.919

5.  Functional Cross-Talk of MbtH-Like Proteins During Thaxtomin Biosynthesis in the Potato Common Scab Pathogen Streptomyces scabiei.

Authors:  Yuting Li; Kapil Tahlan; Dawn R D Bignell
Journal:  Front Microbiol       Date:  2020-10-15       Impact factor: 5.640
  5 in total

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