Huseyin Eren1, Hasan Riza Aydin2, Levent Tumkaya3, Ilke Onur Kazaz4, Yildiray Kalkan3, Seher Nazli Kazaz5, Tolga Mercantepe3, Mustafa Ozan Horsanali6, Adnan Yilmaz7. 1. a Urology Department , Recep Tayyip Erdogan University School of Medicine , Rize , Turkey. 2. b Urology Department, Kanuni Training and Research Hospital , Medical Science University School of Medicine , Trabzon , Turkey. 3. c Histology and Embryology Department , Recep Tayyip Erdogan University School of Medicine , Rize , Turkey. 4. d Urology Department, School of Medicine , Karadeniz Technical University , Trabzon , Turkey. 5. e Medical Oncology Department , Kanuni Training and Research Hospital , Trabzon , Turkey. 6. f Urology Department , Recep Tayyip Erdogan University Training and Research Hospital , Rize , Turkey. 7. g Biochemistry Department , Recep Tayyip Erdogan University School of Medicine , Rize , Turkey.
Abstract
PURPOSE: This study investigated the antioxidant effects of whortleberry against cisplatin-induced nephrotoxicity in rats. MATERIAL AND METHODS: This study included 48 female Sprague-Dawley rats weighing 263.68 ± 8.29 g. The rats were divided into the following six groups, with eight rats in each group: control, ethanol control, whortleberry control, cisplatin control, 16 mg/kg cisplatin +100 mg/kg whortleberry, and 16 mg/kg cisplatin +200 mg/kg whortleberry groups. Biochemical analysis was performed by measuring total oxidant status and total antioxidant status, histopathological analysis was performed by calculating proximal and distal tubule areas (μm2), and immunohistochemical analysis was performed by determining anti-Caspase-3 immunostaining. Differences among the groups were examined using one-way analysis of variance, and p < .05 was considered statistically significant. RESULTS: Cisplatin treatment decreased the total antioxidant status and increased the total oxidant status and Caspase-3 level. Moreover, it resulted in the dilatation, vacuolization and loss of tubular epithelial cells; and glomerular degeneration and edema in the kidney tissues (p < .05). Treatment with 100 and 200 mg whortleberries increased the total antioxidant status; decreased the total oxidant status and Caspase-3 level and ameliorated distal and proximal tubule degeneration, glomerular degeneration and edema in the kidney tissues (p < .05). CONCLUSIONS: Our results indicate that the antioxidant effects of the whortleberry decrease cisplatin-associated nephrotoxicity.
PURPOSE: This study investigated the antioxidant effects of whortleberry against cisplatin-induced nephrotoxicity in rats. MATERIAL AND METHODS: This study included 48 female Sprague-Dawley rats weighing 263.68 ± 8.29 g. The rats were divided into the following six groups, with eight rats in each group: control, ethanol control, whortleberry control, cisplatin control, 16 mg/kg cisplatin +100 mg/kg whortleberry, and 16 mg/kg cisplatin +200 mg/kg whortleberry groups. Biochemical analysis was performed by measuring total oxidant status and total antioxidant status, histopathological analysis was performed by calculating proximal and distal tubule areas (μm2), and immunohistochemical analysis was performed by determining anti-Caspase-3 immunostaining. Differences among the groups were examined using one-way analysis of variance, and p < .05 was considered statistically significant. RESULTS:Cisplatin treatment decreased the total antioxidant status and increased the total oxidant status and Caspase-3 level. Moreover, it resulted in the dilatation, vacuolization and loss of tubular epithelial cells; and glomerular degeneration and edema in the kidney tissues (p < .05). Treatment with 100 and 200 mg whortleberries increased the total antioxidant status; decreased the total oxidant status and Caspase-3 level and ameliorated distal and proximal tubule degeneration, glomerular degeneration and edema in the kidney tissues (p < .05). CONCLUSIONS: Our results indicate that the antioxidant effects of the whortleberry decrease cisplatin-associated nephrotoxicity.
Entities:
Keywords:
Cisplatin; nephrotoxicity; kidney; total antioxidant status; total oxidant status; whortleberry
Authors: A Yilmaz; L Tumkaya; K Akyildiz; Y Kalkan; A F Bodur; F Sargin; H Efe; H A Uydu; Z A Yazici Journal: J Matern Fetal Neonatal Med Date: 2016-08-10