| Literature DB >> 30127505 |
Isaac Canals1,2,3, Aurélie Ginisty1,2,3, Ella Quist1,2,3, Raissa Timmerman1,2,3, Jonas Fritze1,2,3, Giedre Miskinyte2,3,4, Emanuela Monni2,3,4, Marita G Hansen3, Isabel Hidalgo3,5, David Bryder3,5, Johan Bengzon3,6, Henrik Ahlenius7,8,9.
Abstract
The derivation of astrocytes from human pluripotent stem cells is currently slow and inefficient. We demonstrate that overexpression of the transcription factors SOX9 and NFIB in human pluripotent stem cells rapidly and efficiently yields homogeneous populations of induced astrocytes. In our study these cells exhibited molecular and functional properties resembling those of adult human astrocytes and were deemed suitable for disease modeling. Our method provides new possibilities for the study of human astrocytes in health and disease.Entities:
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Year: 2018 PMID： 30127505 DOI： 10.1038/s41592-018-0103-2
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547