| Literature DB >> 30122351 |
Zbigniew Warkocki1, Paweł S Krawczyk2, Dorota Adamska2, Krystian Bijata2, Jose L Garcia-Perez3, Andrzej Dziembowski4.
Abstract
LINE-1 retrotransposition is tightly restricted by layers of regulatory control, with epigenetic pathways being the best characterized. Looking at post-transcriptional regulation, we now show that LINE-1 mRNA 3' ends are pervasively uridylated in various human cellular models and in mouse testes. TUT4 and TUT7 uridyltransferases catalyze the modification and function in cooperation with the helicase/RNPase MOV10 to counteract the RNA chaperone activity of the L1-ORF1p retrotransposon protein. Uridylation potently restricts LINE-1 retrotransposition by a multilayer mechanism depending on differential subcellular localization of the uridyltransferases. We propose that uridine residues added by TUT7 in the cytoplasm inhibit initiation of reverse transcription of LINE-1 mRNAs once they are reimported to the nucleus, whereas uridylation by TUT4, which is enriched in cytoplasmic foci, destabilizes mRNAs. These results provide a model for the post-transcriptional restriction of LINE-1, revealing a key physiological role for TUT4/7-mediated uridylation in maintaining genome stability.Entities:
Keywords: 3′ RACE-seq; L1-ORF1p; L1-ORF2p; LINE-1; MOV10; TUT4; TUT7; poly(A); retrotransposition; uridylation
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Year: 2018 PMID： 30122351 PMCID： PMC6191937 DOI： 10.1016/j.cell.2018.07.022
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582