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Literature DB >> 3010906

Cleavage maps of human cytomegalovirus genome (strain Towne) determined by the use of cosmid cloning system.

Abstract

Virion DNA of human cytomegalovirus strain Towne was partially digested with endonuclease Hind III and fragments larger than 29 kbp were ligated to cosmid pHC79. The whole viral DNA sequence has been cloned in large overlapping segments carried by 32 recombinant cosmid clones (a pIT series). A whole set of Hind III fragments has also been cloned (a pHI series). By using these, we have constructed corrected cleavage maps of strain Towne DNA for Hind III, Bam H I, EcoR I and Xba I.

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Year: 1986 PMID： 3010906 DOI： 10.1007/bf01310878

Source DB: PubMed Journal: Arch Virol ISSN： 0304-8608 Impact factor: 2.574

15 in total

1. DNA of human cytomegalovirus: size heterogeneity and defectiveness resulting from serial undiluted passage.

Authors: M F Stinski; E S Mocarski; D R Thomsen
Journal: J Virol Date: 1979-07 Impact factor: 5.103

2. Size and complexity of human cytomegalovirus DNA.

Authors: J M DeMarchi; M L Blankenship; G D Brown; A S Kaplan
Journal: Virology Date: 1978-09 Impact factor: 3.616

3. Human cytomegalovirus DNA. I. Molecular weight and infectivity.

Authors: J L Geelen; C Walig; P Wertheim; J van der Noordaa
Journal: J Virol Date: 1978-06 Impact factor: 5.103

4. Temperature-sensitive mutants of human cytomegalovirus: isolation and partial characterization of DNA-minus mutants.

Authors: S Ihara; K Hirai; Y Watanabe
Journal: Virology Date: 1978-01 Impact factor: 3.616

5. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

6. A small cosmid for efficient cloning of large DNA fragments.

Authors: B Hohn; J Collins
Journal: Gene Date: 1980-11 Impact factor: 3.688

7. Human cytomegalovirus DNA: restriction enzyme cleavage maps and map locations for immediate-early, early, and late RNAs.

Authors: J M Demarchi
Journal: Virology Date: 1981-10-15 Impact factor: 3.616

Cleavage maps of human cytomegalovirus genome (strain Towne) determined by the use of cosmid cloning system.

1. DNA of human cytomegalovirus: size heterogeneity and defectiveness resulting from serial undiluted passage.

2. Size and complexity of human cytomegalovirus DNA.

3. Human cytomegalovirus DNA. I. Molecular weight and infectivity.

4. Temperature-sensitive mutants of human cytomegalovirus: isolation and partial characterization of DNA-minus mutants.

5. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

6. A small cosmid for efficient cloning of large DNA fragments.

7. Human cytomegalovirus DNA: restriction enzyme cleavage maps and map locations for immediate-early, early, and late RNAs.

8. Cloning of the complete human cytomegalovirus genome in cosmids.

9. Plasmids useable as gene-cloning vectors in an in vitro packaging by coliphage lambda: "cosmids".

10. Use of recombinant plasmids to investigate the structure of the human cytomegalovirus genome.

1. Insertion and deletion mutagenesis of the human cytomegalovirus genome.

2. Identification of mutation sites of a temperature-sensitive mutant of HCMV DNA polymerase activity.

3. Cosmid cloning and restriction endonuclease mapping of the herpesvirus of turkeys (HVT) genome.