Literature DB >> 6286417

Cloning of the complete human cytomegalovirus genome in cosmids.

B Fleckenstein, I Müller, J Collins.   

Abstract

Purified virion DNA (155 X 10(6) Mr) of human cytomegalovirus (CMV) strain Ad169 was partially cleaved with restriction endonucleases HindIII and EcoRI and cloned in the respective cleavage sites of cosmid pHC79. A complete gene library was established in a set of clones containing the viral DNA in long overlapping segments. Restriction maps for HindIII (29 fragments) and EcoRI (36 fragments) were constructed from the linkage of cosmid-cloned fragments, from double digestions of cloned DNA, and from blot hybridization of labeled cloned viral DNA with restriction fragments of virion DNA and singly or doubly cleaved cosmid clones.

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Year:  1982        PMID： 6286417     DOI： 10.1016/0378-1119(82)90054-3

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  91 in total

1.  Recombinant green fluorescent protein-expressing human cytomegalovirus as a tool for screening antiviral agents.

Authors:  M Marschall; M Freitag; S Weiler; G Sorg; T Stamminger
Journal:  Antimicrob Agents Chemother       Date:  2000-06       Impact factor: 5.191

2.  A cytomegalovirus-encoded mitochondria-localized inhibitor of apoptosis structurally unrelated to Bcl-2.

Authors:  V S Goldmacher; L M Bartle; A Skaletskaya; C A Dionne; N L Kedersha; C A Vater; J W Han; R J Lutz; S Watanabe; E D Cahir McFarland; E D Kieff; E S Mocarski; T Chittenden
Journal:  Proc Natl Acad Sci U S A       Date:  1999-10-26       Impact factor: 11.205

3.  A cytomegalovirus-encoded inhibitor of apoptosis that suppresses caspase-8 activation.

Authors:  A Skaletskaya; L M Bartle; T Chittenden; A L McCormick; E S Mocarski; V S Goldmacher
Journal:  Proc Natl Acad Sci U S A       Date:  2001-06-26       Impact factor: 11.205

4.  Fast screening procedures for random transposon libraries of cloned herpesvirus genomes: mutational analysis of human cytomegalovirus envelope glycoprotein genes.

Authors:  U Hobom; W Brune; M Messerle; G Hahn; U H Koszinowski
Journal:  J Virol       Date:  2000-09       Impact factor: 5.103

5.  Low predictive value of polymerase chain reaction for diagnosis of cytomegalovirus disease in liver transplant recipients.

Authors:  R Delgado; C Lumbreras; C Alba; M A Pedraza; J R Otero; R Gómez; E Moreno; A R Noriega; C V Payá
Journal:  J Clin Microbiol       Date:  1992-07       Impact factor: 5.948

6.  Large-scale screening of human sera with cytomegalovirus recombinant antigens.

Authors:  M P Landini; M X Guan; G Jahn; W Lindenmaier; M Mach; A Ripalti; A Necker; T Lazzarotto; B Plachter
Journal:  J Clin Microbiol       Date:  1990-06       Impact factor: 5.948

7.  Cloning and characterization of major antigenic determinants of human cytomegalovirus Ad169 seen by the human immune system.

Authors:  W Lindenmaier; A Necker; S Krause; R Bonewald; J Collins
Journal:  Arch Virol       Date:  1990       Impact factor: 2.574

8.  UL69 of human cytomegalovirus, an open reading frame with homology to ICP27 of herpes simplex virus, encodes a transactivator of gene expression.

Authors:  M Winkler; S A Rice; T Stamminger
Journal:  J Virol       Date:  1994-06       Impact factor: 5.103

9.  The Abundant Tegument Protein pUL25 of Human Cytomegalovirus Prevents Proteasomal Degradation of pUL26 and Supports Its Suppression of ISGylation.

Authors:  Christine Zimmermann; Nicole Büscher; Steffi Krauter; Nadine Krämer; Uwe Wolfrum; Elisabeth Sehn; Stefan Tenzer; Bodo Plachter
Journal:  J Virol       Date:  2018-11-27       Impact factor: 5.103

10.  Cosmid cloning and restriction endonuclease mapping of the herpesvirus of turkeys (HVT) genome.

Authors:  W Lindenmaier; H J Bauer
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574
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