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Literature DB >> 30108705

Discovery of selective cystathionine β-synthase inhibitors by high-throughput screening with a fluorescent thiol probe.

Weining Niu¹, Ping Wu¹, Fei Chen¹, Jun Wang¹, Xiaoya Shang¹, Chunlan Xu¹.

Abstract

A high-throughput assay was developed to identify inhibitors of cystathionine β-synthase (CBS), which is one of three key enzymes involved in H2S biosynthesis. Screening of 6491 natural compounds identified several selective CBS inhibitors, which suppressed the proliferation of HT29 cancer cells, with IC50 values of less than 10 μM.

Entities: Chemical Disease Gene

Year: 2016 PMID： 30108705 PMCID： PMC6072345 DOI： 10.1039/c6md00493h

Source DB: PubMed Journal: Medchemcomm ISSN： 2040-2503 Impact factor: 3.597

22 in total

Review 1. Cystathionine beta-synthase: structure, function, regulation, and location of homocystinuria-causing mutations.

Authors: Edith Wilson Miles; Jan P Kraus
Journal: J Biol Chem Date: 2004-04-15 Impact factor: 5.157

2. Cytotoxic biflavones from Stellera chamaejasme.

Authors: Zhi-xin Wang; Meng-chun Cheng; Xiao-zhe Zhang; Zhi-lai Hong; Ming-zhe Gao; Xiao-xi Kan; Qi Li; Ya-jie Wang; Xiao-xin Zhu; Hong-bin Xiao
Journal: Fitoterapia Date: 2014-10-12 Impact factor: 2.882

3. Tumor-derived hydrogen sulfide, produced by cystathionine-β-synthase, stimulates bioenergetics, cell proliferation, and angiogenesis in colon cancer.

Authors: Csaba Szabo; Ciro Coletta; Celia Chao; Katalin Módis; Bartosz Szczesny; Andreas Papapetropoulos; Mark R Hellmich
Journal: Proc Natl Acad Sci U S A Date: 2013-07-08 Impact factor: 11.205

Review 4. Cystathionine beta-synthase mutations in homocystinuria.

Authors: J P Kraus; M Janosík; V Kozich; R Mandell; V Shih; M P Sperandeo; G Sebastio; R de Franchis; G Andria; L A Kluijtmans; H Blom; G H Boers; R B Gordon; P Kamoun; M Y Tsai; W D Kruger; H G Koch; T Ohura; M Gaustadnes
Journal: Hum Mutat Date: 1999 Impact factor: 4.878

Review 5. Enzymology of H2S biogenesis, decay and signaling.

Authors: Omer Kabil; Ruma Banerjee
Journal: Antioxid Redox Signal Date: 2013-06-07 Impact factor: 8.401

6. Purification and characterization of the wild type and truncated human cystathionine beta-synthase enzymes expressed in E. coli.

Authors: Nina Frank; Jana O Kent; Markus Meier; Jan P Kraus
Journal: Arch Biochem Biophys Date: 2007-11-17 Impact factor: 4.013

7. S-glutathionylation enhances human cystathionine β-synthase activity under oxidative stress conditions.

Authors: Wei-Ning Niu; Pramod Kumar Yadav; Jiri Adamec; Ruma Banerjee
Journal: Antioxid Redox Signal Date: 2014-07-29 Impact factor: 8.401

8. Structural basis for the inhibition mechanism of human cystathionine gamma-lyase, an enzyme responsible for the production of H(2)S.

Authors: Qingxiang Sun; Ruairi Collins; Shufen Huang; Lovisa Holmberg-Schiavone; Ganesh S Anand; Choon-Hong Tan; Susanne van-den-Berg; Lih-Wen Deng; Philip K Moore; Tobias Karlberg; J Sivaraman
Journal: J Biol Chem Date: 2008-11-19 Impact factor: 5.157

9. Identification of cystathionine β-synthase inhibitors using a hydrogen sulfide selective probe.

Authors: Megan K Thorson; Tomas Majtan; Jan P Kraus; Amy M Barrios
Journal: Angew Chem Int Ed Engl Date: 2013-03-19 Impact factor: 15.336

10. Selectivity of commonly used pharmacological inhibitors for cystathionine β synthase (CBS) and cystathionine γ lyase (CSE).

Authors: Antonia Asimakopoulou; Panagiotis Panopoulos; Christos T Chasapis; Ciro Coletta; Zongmin Zhou; Giuseppe Cirino; Athanassios Giannis; Csaba Szabo; Georgios A Spyroulias; Andreas Papapetropoulos
Journal: Br J Pharmacol Date: 2013-06 Impact factor: 8.739

8 in total

Review 1. Cystathionine-β-Synthase: Molecular Regulation and Pharmacological Inhibition.

Authors: Karim Zuhra; Fiona Augsburger; Tomas Majtan; Csaba Szabo
Journal: Biomolecules Date: 2020-04-30

Review 2. Emerging roles of cystathionine β-synthase in various forms of cancer.

Authors: Kelly Ascenção; Csaba Szabo
Journal: Redox Biol Date: 2022-05-10 Impact factor: 10.787

3. The CBS-H₂S axis promotes liver metastasis of colon cancer by upregulating VEGF through AP-1 activation.

Authors: Shihao Guo; Jichang Li; Zhihao Huang; Taohua Yue; Jing Zhu; Xin Wang; Yucun Liu; Pengyuan Wang; Shanwen Chen
Journal: Br J Cancer Date: 2021-12-24 Impact factor: 9.075

4. Screening Pyridine Derivatives against Human Hydrogen Sulfide-synthesizing Enzymes by Orthogonal Methods.

Authors: Karim Zuhra; Pedro M F Sousa; Giulia Paulini; Ana Rita Lemos; Zenta Kalme; Imants Bisenieks; Egils Bisenieks; Brigita Vigante; Gunars Duburs; Tiago M Bandeiras; Luciano Saso; Alessandro Giuffrè; João B Vicente
Journal: Sci Rep Date: 2019-01-24 Impact factor: 4.379

Discovery of selective cystathionine β-synthase inhibitors by high-throughput screening with a fluorescent thiol probe.

Review 1. Cystathionine beta-synthase: structure, function, regulation, and location of homocystinuria-causing mutations.

2. Cytotoxic biflavones from Stellera chamaejasme.

3. Tumor-derived hydrogen sulfide, produced by cystathionine-β-synthase, stimulates bioenergetics, cell proliferation, and angiogenesis in colon cancer.

Review 4. Cystathionine beta-synthase mutations in homocystinuria.

Review 5. Enzymology of H2S biogenesis, decay and signaling.

6. Purification and characterization of the wild type and truncated human cystathionine beta-synthase enzymes expressed in E. coli.

7. S-glutathionylation enhances human cystathionine β-synthase activity under oxidative stress conditions.

8. Structural basis for the inhibition mechanism of human cystathionine gamma-lyase, an enzyme responsible for the production of H(2)S.

9. Identification of cystathionine β-synthase inhibitors using a hydrogen sulfide selective probe.

10. Selectivity of commonly used pharmacological inhibitors for cystathionine β synthase (CBS) and cystathionine γ lyase (CSE).

Review 1. Cystathionine-β-Synthase: Molecular Regulation and Pharmacological Inhibition.

Review 2. Emerging roles of cystathionine β-synthase in various forms of cancer.

3. The CBS-H₂S axis promotes liver metastasis of colon cancer by upregulating VEGF through AP-1 activation.

4. Screening Pyridine Derivatives against Human Hydrogen Sulfide-synthesizing Enzymes by Orthogonal Methods.

5. Therapeutic potential of endogenous hydrogen sulfide inhibition in breast cancer (Review).

Review 6. Methods for Suppressing Hydrogen Sulfide in Biological Systems.

Review 7. The Hidden Role of Hydrogen Sulfide Metabolism in Cancer.

Review 8. Moving Past Quinone-Methides: Recent Advances Toward Minimizing Electrophilic Byproducts from COS/H₂S Donors.

Review 1. Cystathionine beta-synthase: structure, function, regulation, and location of homocystinuria-causing mutations.

Review 4. Cystathionine beta-synthase mutations in homocystinuria.

Review 5. Enzymology of H2S biogenesis, decay and signaling.

Review 1. Cystathionine-β-Synthase: Molecular Regulation and Pharmacological Inhibition.

Review 2. Emerging roles of cystathionine β-synthase in various forms of cancer.

Review 6. Methods for Suppressing Hydrogen Sulfide in Biological Systems.

Review 7. The Hidden Role of Hydrogen Sulfide Metabolism in Cancer.

Review 8. Moving Past Quinone-Methides: Recent Advances Toward Minimizing Electrophilic Byproducts from COS/H2S Donors.

Review 8. Moving Past Quinone-Methides: Recent Advances Toward Minimizing Electrophilic Byproducts from COS/H₂S Donors.