Yuhan Liu1,1, Juan Zhang2,1, Cuihong Xing3, Shuxin Wei4, Na Guo5, Yanli Wang6. 1. The First Department of Orthopedics, Qingdao West Coast New Area Central Hospital, Qingdao, Shandong, China. 2. Outpatient Department, Yantai Yeda Hospital, Yantai, Shandong, China. 3. Department of Internal Medicine Ward, The People's Hospital of Zhangqiu Area, Jinan, Shandong, China. 4. Department of Anesthesiology, The People's Hospital of Zhangqiu Area, Jinan, Shandong, China. 5. Department of Blood Transfusion, The People's Hospital of Zhangqiu Area, Jinan, Shandong, China. 6. Department of Oncology, Jining No. 1 People's Hospital, Jining, Shandong, China.
Abstract
OBJECTIVE: Osteosarcoma is the most common malignant tumor of bone with high recurrent rate. miR-486 was downregulated and acted as a tumor suppressor in plenty of tumors. The purpose of this study was to explore how miR-486 worked in osteosarcoma on cell invasion and EMT. RESULTS: miR-486 was low expressed in osteosarcoma while PIM1 was overexpressed, and it had negative correlation between miR-486 and PIM1. miR-486 upregulation or PIM1 downregulation could inhibit osteosarcoma cell invasion and EMT. Meanwhile, miR-486 mediated PIM1 expression through binding to PIM1 mRNA 3'-UTR. PIM1 could reveal partial function of miR-486 on osteosarcoma invasion. In addition, miR-486 low expression or PIM1 overexpression predicted poor prognosis of osteosarcoma patients. CONCLUSION: miR-486 regulated osteosarcoma cell invasion and EMT through targeting to PIM1. miR-486 low expression or PIM1 overexpression predicted poor prognosis of osteosarcoma patients. The newly identified miR-486/PIM1 axis provides novel insight into the pathogenesis of osteosarcoma.
OBJECTIVE:Osteosarcoma is the most common malignant tumor of bone with high recurrent rate. miR-486 was downregulated and acted as a tumor suppressor in plenty of tumors. The purpose of this study was to explore how miR-486 worked in osteosarcoma on cell invasion and EMT. RESULTS:miR-486 was low expressed in osteosarcoma while PIM1 was overexpressed, and it had negative correlation between miR-486 and PIM1. miR-486 upregulation or PIM1 downregulation could inhibit osteosarcoma cell invasion and EMT. Meanwhile, miR-486 mediated PIM1 expression through binding to PIM1 mRNA 3'-UTR. PIM1 could reveal partial function of miR-486 on osteosarcoma invasion. In addition, miR-486 low expression or PIM1 overexpression predicted poor prognosis of osteosarcomapatients. CONCLUSION:miR-486 regulated osteosarcoma cell invasion and EMT through targeting to PIM1. miR-486 low expression or PIM1 overexpression predicted poor prognosis of osteosarcomapatients. The newly identified miR-486/PIM1 axis provides novel insight into the pathogenesis of osteosarcoma.