Literature DB >> 3007654

Transfection of Corynebacterium lilium protoplasts.

P Yeh, J Oreglia, A M Sicard.   

Abstract

A protoplast transfection system has been developed for a lysine-producing bacterium, Corynebacterium lilium, using the DNA of phage CL31. Phage CL31 is lytic and specific to C. lilium and has a genome of approximately 48 kb. The transfection procedure involves a polyethylene-glycol-mediated introduction of the DNA into lysozyme-treated cells and has a maximum efficiency of 3 X 10(4) transfectants per microgram DNA.

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Year:  1985        PMID： 3007654     DOI： 10.1099/00221287-131-12-3179

Source DB:  PubMed          Journal:  J Gen Microbiol        ISSN： 0022-1287


  7 in total

1.  Isolation and preliminary characterization of twenty bacteriophages infecting either brevibacterium or arthrobacter strains.

Authors:  A Trautwetter; C Blanco
Journal:  Appl Environ Microbiol       Date:  1988-06       Impact factor: 4.792

2.  Increased fertility of Corynebacterium glutamicum recipients in intergeneric matings with Escherichia coli after stress exposure.

Authors:  A Schäfer; J Kalinowski; A Pühler
Journal:  Appl Environ Microbiol       Date:  1994-02       Impact factor: 4.792

3.  Structural characteristics of the Corynebacterium lilium bacteriophage CL31.

Authors:  A Trautwetter; C Blanco; A M Sicard
Journal:  J Virol       Date:  1987-05       Impact factor: 5.103

4.  Cloning and characterization of a DNA region encoding a stress-sensitive restriction system from Corynebacterium glutamicum ATCC 13032 and analysis of its role in intergeneric conjugation with Escherichia coli.

Authors:  A Schäfer; A Schwarzer; J Kalinowski; A Pühler
Journal:  J Bacteriol       Date:  1994-12       Impact factor: 3.490

5.  Genetic characterization of site-specific integration functions of phi AAU2 infecting "Arthrobacter aureus" C70.

Authors:  C Le Marrec; S Moreau; S Loury; C Blanco; A Trautwetter
Journal:  J Bacteriol       Date:  1996-04       Impact factor: 3.490

6.  Electrotransformation of intact cells of Brevibacterium flavum MJ-233.

Authors:  Y Satoh; K Hatakeyama; K Kohama; M Kobayashi; Y Kurusu; H Yukawa
Journal:  J Ind Microbiol       Date:  1990 Apr-May

7.  Genome Sequence of the Bacteriophage CL31 and Interaction with the Host Strain Corynebacterium glutamicum ATCC 13032.

Authors:  Max Hünnefeld; Ulrike Viets; Vikas Sharma; Astrid Wirtz; Aël Hardy; Julia Frunzke
Journal:  Viruses       Date:  2021-03-17       Impact factor: 5.048
  7 in total

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