| Literature DB >> 30070754 |
Manabendra Das1,2, Yang Du3, Jonas S Mortensen4, Parameswaran Hariharan5, Hyun Sung Lee1, Bernadette Byrne6, Claus J Loland4, Lan Guan5, Brian K Kobilka3, Pil Seok Chae1.
Abstract
A new family of tandem facial glucosides/maltosides (TFGs/TFMs) for membrane protein manipulation was prepared. The best detergent varied depending on the hydrophobic thickness of the target protein, but ether-based TFMs (TFM-C0E, TFM-C3E, and TFM-C5E) were notable for their ability to confer higher membrane protein stability than the previously developed amide-based TFA-1 (P. S. Chae, K. Gotfryd, J. Pacyna, L. J. W. Miercke, S. G. F. Rasmussen, R. A. Robbins, R. R. Rana, C. J. Loland, B. Kobilka, R. Stroud, B. Byrne, U. Gether, S. H. Gellman, J. Am. Chem. Soc. 2010, 132, 16750-16752). Thus, this study not only introduces novel agents with the potential to be used in membrane protein research but also highlights the importance of both the hydrophobic length and linker functionality of the detergent in stabilizing membrane proteins.Entities:
Keywords: amphiphiles; detergent design; detergent faciality; protein stabilization; protein structures
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Year: 2018 PMID： 30070754 PMCID： PMC7233144 DOI： 10.1002/cbic.201800388
Source DB: PubMed Journal: Chembiochem ISSN： 1439-4227 Impact factor: 3.164