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Literature DB >> 3006586

Rapid physical mapping by transposon Tn5 mutagenesis to localize the cloned yeast ILV2 gene.

Abstract

A rapid method for Tn5 mutagenesis of cloned genes on multicopy plasmids was used to map a yeast ILV2 mutant allele encoding a sulfometuron methyl-resistant acetolactate synthase. Twenty-one of 40 independent Tn5 insertions were within the 5.6-kilobase-pair cloned segment. Of these, seven adjacent transposition events inactivated the sulfometuron methyl resistance determinant, localizing the ILV2 gene to a minimum 1.4-kilobase-pair region.

Entities: Gene Species

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Year: 1986 PMID： 3006586 PMCID： PMC238841 DOI： 10.1128/aem.51.1.206-208.1986

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

15 in total

1. Acetolactate synthase is the site of action of two sulfonylurea herbicides in higher plants.

Authors: R S Chaleff; C J Mauvais
Journal: Science Date: 1984-06-29 Impact factor: 47.728

2. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.

Authors: M Dagert; S D Ehrlich
Journal: Gene Date: 1979-05 Impact factor: 3.688

3. A complementation analysis of the restriction and modification of DNA in Escherichia coli.

Authors: H W Boyer; D Roulland-Dussoix
Journal: J Mol Biol Date: 1969-05-14 Impact factor: 5.469

4. Rapid and efficient cosmid cloning.

Authors: D Ish-Horowicz; J F Burke
Journal: Nucleic Acids Res Date: 1981-07-10 Impact factor: 16.971

5. Control of transposon Tn5 transposition in Escherichia coli.

Authors: C Sasakawa; J B Lowe; L McDivitt; D E Berg
Journal: Proc Natl Acad Sci U S A Date: 1982-12 Impact factor: 11.205

Review 6. The use of transposon Tn5 mutagenesis in the rapid generation of correlated physical and genetic maps of DNA segments cloned into multicopy plasmids--a review.

Authors: F J de Bruijn; J R Lupski
Journal: Gene Date: 1984-02 Impact factor: 3.688

2. Molecular and genetic characterization of SPT4, a gene important for transcription initiation in Saccharomyces cerevisiae.

Authors: E A Malone; J S Fassler; F Winston
Journal: Mol Gen Genet Date: 1993-03

2 in total

Rapid physical mapping by transposon Tn5 mutagenesis to localize the cloned yeast ILV2 gene.

1. Acetolactate synthase is the site of action of two sulfonylurea herbicides in higher plants.

2. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.

3. A complementation analysis of the restriction and modification of DNA in Escherichia coli.

4. Rapid and efficient cosmid cloning.

5. Control of transposon Tn5 transposition in Escherichia coli.

Review 6. The use of transposon Tn5 mutagenesis in the rapid generation of correlated physical and genetic maps of DNA segments cloned into multicopy plasmids--a review.

7. Site of action of chlorsulfuron: inhibition of valine and isoleucine biosynthesis in plants.

8. Escherichia coli K-12 auxotrophs induced by insertion of the transposable element Tn5.

9. Cloning and expression of the fhu genes involved in iron(III)-hydroxamate uptake by Escherichia coli.

10. Genetic analysis of mutants of Saccharomyces cerevisiae resistant to the herbicide sulfometuron methyl.

1. Cloning by gene amplification of two loci conferring multiple drug resistance in Saccharomyces.

2. Molecular and genetic characterization of SPT4, a gene important for transcription initiation in Saccharomyces cerevisiae.