Literature DB >> 30062424

Using GAL4-Inducible Transgenics to Modulate Rho GTPase Activity in Zebrafish.

Nicholas J Hanovice1, Emily McMains2, Jeffrey M Gross3.   

Abstract

Rho GTPases are Ras-family G proteins that regulate many critical cellular functions. Due to their requirement during early embryonic development, investigations into the function of Rho GTPases at a tissue-specific level require inducible and spatially targeted modulation of Rho GTPase activity. Here, we describe the use of ten novel zebrafish transgenics enabling GAL4-specific expression of Rho GTPases to modulate Rho GTPase activity with spatial and temporal control.

Entities:  

Keywords:  Cdc42; GAL4/UAS; Heat shock; Rac1; RhoA; Transgenics; Zebrafish

Mesh：

Substances：

Year:  2018        PMID： 30062424      PMCID： PMC6891047          DOI： 10.1007/978-1-4939-8612-5_24

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  17 in total

Review 1.  Small GTP-binding proteins.

Authors:  Y Takai; T Sasaki; T Matozaki
Journal:  Physiol Rev       Date:  2001-01       Impact factor: 37.312

Review 2.  Rho GTPases in neuronal morphogenesis.

Authors:  L Luo
Journal:  Nat Rev Neurosci       Date:  2000-12       Impact factor: 34.870

Review 3.  Microinjection into zebrafish embryos.

Authors:  Q Xu
Journal:  Methods Mol Biol       Date:  1999

Review 4.  Rho GTPases in cell biology.

Authors:  Sandrine Etienne-Manneville; Alan Hall
Journal:  Nature       Date:  2002-12-12       Impact factor: 49.962

5.  Rac1 is required for the formation of three germ layers during gastrulation.

Authors:  K Sugihara; N Nakatsuji; K Nakamura; K Nakao; R Hashimoto; H Otani; H Sakagami; H Kondo; S Nozawa; A Aiba; M Katsuki
Journal:  Oncogene       Date:  1998-12-31       Impact factor: 9.867

6.  A GAL4-inducible transgenic tool kit for the in vivo modulation of Rho GTPase activity in zebrafish.

Authors:  Nicholas J Hanovice; Emily McMains; Jeffrey M Gross
Journal:  Dev Dyn       Date:  2016-05-17       Impact factor: 3.780

7.  Epidermal transient down-regulation of retinol-binding protein 4 and mirror expression of apolipoprotein Eb and estrogen receptor 2a during zebrafish fin and scale development.

Authors:  Angèle Tingaud-Sequeira; Jean Forgue; Michèle André; Patrick J Babin
Journal:  Dev Dyn       Date:  2006-11       Impact factor: 3.780

8.  Lamina-specific axonal projections in the zebrafish tectum require the type IV collagen Dragnet.

Authors:  Tong Xiao; Herwig Baier
Journal:  Nat Neurosci       Date:  2007-11-04       Impact factor: 24.884

9.  An instructive function for Notch in promoting gliogenesis in the zebrafish retina.

Authors:  N Scheer; A Groth; S Hans; J A Campos-Ortega
Journal:  Development       Date:  2001-04       Impact factor: 6.868

10.  A laser pointer driven microheater for precise local heating and conditional gene regulation in vivo. Microheater driven gene regulation in zebrafish.

Authors:  Mike Placinta; Meng-Chieh Shen; Marc Achermann; Rolf O Karlstrom
Journal:  BMC Dev Biol       Date:  2009-12-30       Impact factor: 1.978
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  1 in total

Review 1.  Rho GTPases Signaling in Zebrafish Development and Disease.

Authors:  Marie-José Boueid; Aya Mikdache; Emilie Lesport; Cindy Degerny; Marcel Tawk
Journal:  Cells       Date:  2020-12-08       Impact factor: 6.600
  1 in total

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