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Literature DB >> 3005650

Lack of evidence for VPg priming of poliovirus RNA synthesis in the host factor-dependent in vitro replicase reaction.

Abstract

Anti-VPg immunoprecipitable RNA labeled in vitro during a poliovirus RNA polymerase reaction was formed by the elongation of VPg-containing template fragments rather than by initiation with VPg. The reaction was dependent on a host factor (terminal uridylyl transferase). The incorporation of labeled UTP could be detected with only the host factor present.

Entities: Chemical Species

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Year: 1986 PMID： 3005650 PMCID： PMC252895

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

16 in total

1. Identification of a protein linked to nascent poliovirus RNA and to the polyuridylic acid of negative-strand RNA.

Authors: R F Pettersson; V Ambros; D Baltimore
Journal: J Virol Date: 1978-08 Impact factor: 5.103

2. Covalent linkage of a protein to a defined nucleotide sequence at the 5'-terminus of virion and replicative intermediate RNAs of poliovirus.

Authors: J B Flanegan; R F Petterson; V Ambros; N J Hewlett; D Baltimore
Journal: Proc Natl Acad Sci U S A Date: 1977-03 Impact factor: 11.205

3. The location of the polio genome protein in viral RNAs and its implication for RNA synthesis.

Authors: A Nomoto; B Detjen; R Pozzatti; E Wimmer
Journal: Nature Date: 1977-07-21 Impact factor: 49.962

4. Dependence of the activity of the poliovirus replicase on the host cell protein.

Authors: A Dasgupta; P Zabel; D Baltimore
Journal: Cell Date: 1980-02 Impact factor: 41.582

5. Isolation of a soluble and template-dependent poliovirus RNA polymerase that copies virion RNA in vitro.

Authors: J B Flanegan; T A Van Dyke
Journal: J Virol Date: 1979-10 Impact factor: 5.103

6. Replication of picornaviruses. I. Evidence from in vitro RNA synthesis that poly(A) of the poliovirus genome is genetically coded.

Authors: K Dorsch-Häsler; Y Yogo; E Wimmer
Journal: J Virol Date: 1975-12 Impact factor: 5.103

2. Characterization of product RNAs synthesized in vitro by poliovirus RNA polymerase purified by chromatography on hydroxylapatite or poly(U) Sepharose.

Authors: D C Young; G J Tobin; J B Flanegan
Journal: J Virol Date: 1987-02 Impact factor: 5.103

3. Host factor-induced template modification during synthesis of poliovirus RNA in vitro.

Authors: T D Hey; O C Richards; E Ehrenfeld
Journal: J Virol Date: 1987-03 Impact factor: 5.103

4. Purification and properties of poliovirus RNA polymerase expressed in Escherichia coli.

Authors: S J Plotch; O Palant; Y Gluzman
Journal: J Virol Date: 1989-01 Impact factor: 5.103

4 in total

Lack of evidence for VPg priming of poliovirus RNA synthesis in the host factor-dependent in vitro replicase reaction.

1. Identification of a protein linked to nascent poliovirus RNA and to the polyuridylic acid of negative-strand RNA.

2. Covalent linkage of a protein to a defined nucleotide sequence at the 5'-terminus of virion and replicative intermediate RNAs of poliovirus.

3. The location of the polio genome protein in viral RNAs and its implication for RNA synthesis.

4. Dependence of the activity of the poliovirus replicase on the host cell protein.

5. Isolation of a soluble and template-dependent poliovirus RNA polymerase that copies virion RNA in vitro.

6. Replication of picornaviruses. I. Evidence from in vitro RNA synthesis that poly(A) of the poliovirus genome is genetically coded.

7. Poliovirus replicase stimulation by terminal uridylyl transferase.

8. Polyadenylic acid at the 3'-terminus of poliovirus RNA.

9. Identification of poliovirus polypeptide P63 as a soluble RNA-dependent RNA polymerase.

10. Genome-length copies of poliovirion RNA are synthesized in vitro by the poliovirus RNA-dependent RNA polymerase.

1. Expression, purification, and properties of recombinant encephalomyocarditis virus RNA-dependent RNA polymerase.

2. Characterization of product RNAs synthesized in vitro by poliovirus RNA polymerase purified by chromatography on hydroxylapatite or poly(U) Sepharose.

3. Host factor-induced template modification during synthesis of poliovirus RNA in vitro.

4. Purification and properties of poliovirus RNA polymerase expressed in Escherichia coli.