Thaer Khoury1, Xuan Peng2, Li Yan2, Dan Wang2, Vidya Nagrale1. 1. Department of Pathology, Roswell Park Cancer Institute, Buffalo, NY. 2. Department of Biostatistics, Roswell Park Cancer Institute, Buffalo, NY.
Abstract
OBJECTIVES: The aims were to evaluate breast cancer (BC) tumor-infiltrating lymphocytes (TILs) interobserver variability, heterogeneity, and the fidelity of scoring TILs in core needle biopsy (CNB). METHODS: Matching CNB and two full-face sections (FFSs) of BC cases (n = 100) were independently reviewed by two pathologists. Percentage of stromal lymphocytes (TIL-str) and intratumoral lymphocytes (iTu-Ly) were recorded. RESULTS: The weighted κ values for the degree of agreement between both raters were 0.53 to 0.71. However, there was a slight improvement in the interobserver variability for TIL-str and slight decline in iTu-Ly when ER+/HER2- cases were excluded. The intraclass correlation coefficient for FFS1 vs FFS2 was 0.91 for TIL-str and 0.96 for iTu-Ly. Spearman correlation coefficient for CNB vs FFS1/FFS2 was 0.81 for TIL-str and 0.79 for iTu-Ly. CONCLUSIONS: We conclude that the agreement in TILs scoring between the raters is acceptable. Caution should be practiced when scoring iTu-Ly in CNBs.
OBJECTIVES: The aims were to evaluate breast cancer (BC) tumor-infiltrating lymphocytes (TILs) interobserver variability, heterogeneity, and the fidelity of scoring TILs in core needle biopsy (CNB). METHODS: Matching CNB and two full-face sections (FFSs) of BC cases (n = 100) were independently reviewed by two pathologists. Percentage of stromal lymphocytes (TIL-str) and intratumoral lymphocytes (iTu-Ly) were recorded. RESULTS: The weighted κ values for the degree of agreement between both raters were 0.53 to 0.71. However, there was a slight improvement in the interobserver variability for TIL-str and slight decline in iTu-Ly when ER+/HER2- cases were excluded. The intraclass correlation coefficient for FFS1 vs FFS2 was 0.91 for TIL-str and 0.96 for iTu-Ly. Spearman correlation coefficient for CNB vs FFS1/FFS2 was 0.81 for TIL-str and 0.79 for iTu-Ly. CONCLUSIONS: We conclude that the agreement in TILs scoring between the raters is acceptable. Caution should be practiced when scoring iTu-Ly in CNBs.
Authors: Iris Nederlof; Davide De Bortoli; Yacine Bareche; Bastien Nguyen; Michiel de Maaker; Gerrit K J Hooijer; Laurence Buisseret; Marleen Kok; Marcel Smid; Gert G G M Van den Eynden; Arie B Brinkman; Jan Hudecek; Jan Koster; Christos Sotiriou; Denis Larsimont; John W M Martens; Marc J van de Vijver; Hugo M Horlings; Roberto Salgado; Elia Biganzoli; Christine Desmedt Journal: Breast Cancer Res Date: 2019-12-26 Impact factor: 6.466
Authors: Serdar Altinay; Laurent Arnould; Maschenka Balkenhol; Glenn Broeckx; Octavio Burguès; Cecile Colpaert; Franceska Dedeurwaerdere; Benjamin Dessauvagie; Valérie Duwel; Giuseppe Floris; Stephen Fox; Clara Gerosa; Delfyne Hastir; Shabnam Jaffer; Eline Kurpershoek; Magali Lacroix-Triki; Andoni Laka; Kathleen Lambein; Gaëtan Marie MacGrogan; Caterina Marchiò; Maria-Dolores Martin Martinez; Sharon Nofech-Mozes; Dieter Peeters; Alberto Ravarino; Emily Reisenbichler; Erika Resetkova; Souzan Sanati; Anne-Marie Schelfhout; Vera Schelfhout; Abeer Shaaban; Renata Sinke; Claudia M Stanciu-Pop; Carolien H M van Deurzen; Koen K Van de Vijver; Anne-Sophie Van Rompuy; Anne Vincent-Salomon; Hannah Y Wen; Serena Wong; Mieke R Van Bockstal; Aline François; Caroline Bouzin; Christine Galant Journal: Mod Pathol Date: 2021-07-03 Impact factor: 7.842