BACKGROUND/AIMS: Lysyl oxidase (LOX) family members play a key role in modifying the primary tumor microenvironment by crosslinking collagens and elastin in the extracellular matrix. The aim of this study was to analyze the LOX-like (LOXL)1, LOXL3, and LOXL4 expressions in gastric cancer tissue by immunohistochemical staining. METHODS: The correlations between the clinicopathological features of 597 primary gastric carcinomas and LOX family members - LOXL1, LOXL3, and LOXL4 - were investigated by immunohistochemical studies. The effect of the transforming growth -factor β1 (TGFβ1) on the expressions of LOXL1, LOXL3, and LOXL4 in gastric cancer was examined using diffuse-type gastric cancer cell lines in vitro. RESULTS: The expressions of LOXL1, LOXL3, and LOXL4 were correlated with T invasion, lymph node metastasis, and lymphatic and venous invasion. LOXL1 expression was associated with histological intestinal-type and expanding growth patterns. The overall survival of patients with LOXL1-, LOXL3-, or LOXL4-positive cancer was poorer than those with negative cancer. LOXL3 and LOXL4 mRNA expressions were significantly high in diffuse-type gastric cancer cells with high invasion ability. TGFβ decreased the LOXL1 expression and increased LOXL3 and LOXL4 expression. CONCLUSION: LOXL1, LOXL3, and LOXL4 expressions are associated with distant metastasis of gastric cancer.
BACKGROUND/AIMS: Lysyl oxidase (LOX) family members play a key role in modifying the primary tumor microenvironment by crosslinking collagens and elastin in the extracellular matrix. The aim of this study was to analyze the LOX-like (LOXL)1, LOXL3, and LOXL4 expressions in gastric cancer tissue by immunohistochemical staining. METHODS: The correlations between the clinicopathological features of 597 primary gastric carcinomas and LOX family members - LOXL1, LOXL3, and LOXL4 - were investigated by immunohistochemical studies. The effect of the transforming growth -factor β1 (TGFβ1) on the expressions of LOXL1, LOXL3, and LOXL4 in gastric cancer was examined using diffuse-type gastric cancer cell lines in vitro. RESULTS: The expressions of LOXL1, LOXL3, and LOXL4 were correlated with T invasion, lymph node metastasis, and lymphatic and venous invasion. LOXL1 expression was associated with histological intestinal-type and expanding growth patterns. The overall survival of patients with LOXL1-, LOXL3-, or LOXL4-positive cancer was poorer than those with negative cancer. LOXL3 and LOXL4 mRNA expressions were significantly high in diffuse-type gastric cancer cells with high invasion ability. TGFβ decreased the LOXL1 expression and increased LOXL3 and LOXL4 expression. CONCLUSION:LOXL1, LOXL3, and LOXL4 expressions are associated with distant metastasis of gastric cancer.
Authors: Lei Zhao; Haiya Niu; Yutao Liu; Lei Wang; Ning Zhang; Gaiqiang Zhang; Rongqing Liu; Mei Han Journal: J Cancer Date: 2019-10-20 Impact factor: 4.207
Authors: Deborah A Smithen; Leo M H Leung; Mairi Challinor; Rae Lawrence; HaoRan Tang; Dan Niculescu-Duvaz; Simon P Pearce; Robert Mcleary; Filipa Lopes; Mohammed Aljarah; Michael Brown; Louise Johnson; Graeme Thomson; Richard Marais; Caroline Springer Journal: J Med Chem Date: 2019-09-04 Impact factor: 7.446
Authors: Victoria Harle; Gemma Turner; Louise van der Weyden; Victoria Offord; Vivek Iyer; Alastair Droop; Agnieszka Swiatkowska; Roy Rabbie; Andrew D Campbell; Owen J Sansom; Mercedes Pardo; Jyoti S Choudhary; Ingrid Ferreira; Mark Tullett; Mark J Arends; Anneliese O Speak; David J Adams Journal: Commun Biol Date: 2021-03-23