| Literature DB >> 30042597 |
Aiswarya S Panicker1, Syed Azmal Ali2, Santosh Anand1, Narender Raju Panjagari3, Sudarshan Kumar2, A K Mohanty2, Pradip V Behare1.
Abstract
This study aimed to check the in vitro probiotic properties of eleven Lactobacillus fermentum strains previously isolated from fermented dairy products and infant faeces. These cultures were tested for their tolerance to different pH such as 2.0, 2.5, 3.0, 3.5 and 6.5, bile salt hydrolysis and cell surface hydrophobicity. All the strains were persistent at pH 3.5 for 3 h whereas only faecal origin isolates such as L. fermentum BIF-19, BIF-20, BIF-18 and MTCC 8711 had shown considerable growth at pH 2.5. The strains NCDC-400, MTCC 8711, BIF-18, BIF-19 and BIF-20 showed slight to intense precipitation zone of bile salt hydrolase activity by agar plate assay. The strain L. fermentum BIF-19 exhibited best preliminary probiotic properties was selected for the adhesion to Caco-2 cell lines, which shows similar adhesion to that observed for standard probiotic Lactobacillus rhamnosus GG.Entities:
Keywords: Acid tolerance; BSH; Caco-2; Cell adhesion; Lactobacillus fermentum; Probiotic
Year: 2018 PMID： 30042597 PMCID： PMC6033835 DOI： 10.1007/s13197-018-3197-8
Source DB: PubMed Journal: J Food Sci Technol ISSN： 0022-1155 Impact factor: 2.701