Seasonal recovery of Edwardsiella piscicida from wild European eels and natural waters: Isolation methods, virulence and reservoirs.

A total of 127 wild eels caught in the L'Albufera Lake (Spain) and 24 samples of lagoon freshwater were analysed for 1-year period. Edwardsiella strains were isolated from liver/kidney on TSA-1 plates in 31.9% of total diseased specimens, and the edwardsiellosis prevalence in the fishery was of 11.8%. The use of double-strength Salmonella-Shigella (DSSS) broth and SS agar yielded Edwardsiella isolation from intestine in 100% of those edwardsiellosis-diseased eels, but also in 40.4% of other sick fish with vibriosis or aeromonosis and in 28.8% of healthy eels, as well as from freshwater in 8.3% of samples. Pure cultures were isolated on SS agar from the former, but motile Aeromonas, Plesiomonas shigelloides and Hafnia alvei were recovered along with Edwardsiella in the other samples. Edwardsiella isolates identification at species level revealed that E. piscicida was distributed between wild eels and freshwater but E. tarda only did in freshwater. All E. piscicida strains were virulent for eels (LD  < 1.0 × 106 CFU/fish) but that of E. tarda was not. This is the first report of E. piscicida in wild eel intestines and natural freshwater, highlighting its role as potential reservoirs for the bacterium. A seasonal recovery was found for E. piscicida at water temperature above 20°C.