Andrei C Ionescu1, Gloria Cazzaniga2, Marco Ottobelli2, Jack L Ferracane3, Gaetano Paolone4, Eugenio Brambilla2. 1. Department of Biomedical, Surgical and Dental Sciences, IRCCS Galeazzi Orthopedic Institute, University of Milan, Milan, Italy. Electronic address: Andrei.ionescu@unimi.it. 2. Department of Biomedical, Surgical and Dental Sciences, IRCCS Galeazzi Orthopedic Institute, University of Milan, Milan, Italy. 3. Department of Restorative Dentistry, Oregon Health & Science University, Portland, OR, USA. 4. Dental Clinic - Restorative Dentistry, Vita-Salute San Raffaele University, Milan, Italy.
Abstract
OBJECTIVES: The interfacial conditions occurring during light-curing procedures of resin-based composites (RBCs) influence their surface properties and therefore the biological behavior of the material. This study aimed to evaluate the influence of different surface curing conditions on in vitro biofilm formation by Streptococcus mutans and mixed oral microflora, in the presence or absence of surface salivary pre-conditioning. METHODS: Two nanohybrid RBCs and four interfacial curing conditions (open air, argon, nitrogen and glycerin) were evaluated. Surface roughness (SR), surface elemental composition (energy-dispersive X-ray spectrometry, EDS) and biofilm formation (S. mutans and oral microcosm) were assessed. Surfaces were observed using scanning electron microscopy (SEM). Microbiological tests were performed with and without saliva pre-conditioning of the surfaces. EDS analysis was performed before and after biofilm formation, and biofilm morphology was evaluated using confocal laser scanning microscopy (CLSM). Data were analyzed using multi-way ANOVA and Tukey post-hoc test (p < 0.05). RESULTS: Interfacial curing conditions significantly influenced SR depending on the tested RBC. EDS analysis showed that surface elemental composition was significantly influenced by the interfacial curing condition depending on the tested RBC. Interfacial curing conditions significantly influenced biofilm formation in both microbiological models in the absence of saliva pre-conditioning, depending on the tested RBC, whereas saliva pre-conditioning abrogated these effects. CONCLUSIONS: Surface curing conditions significantly impacted biofilm formation in a material-dependent manner, which was abrogated when surfaces were pre-conditioned with saliva. CLINICAL SIGNIFICANCE: Curing under glycerin did not improve the microbiological performances of the tested RBCs. These results, needing to be confirmed by in vivo data, have the potential to simplify operative procedures in restorative dentistry.
OBJECTIVES: The interfacial conditions occurring during light-curing procedures of resin-based composites (RBCs) influence their surface properties and therefore the biological behavior of the material. This study aimed to evaluate the influence of different surface curing conditions on in vitro biofilm formation by Streptococcus mutans and mixed oral microflora, in the presence or absence of surface salivary pre-conditioning. METHODS: Two nanohybrid RBCs and four interfacial curing conditions (open air, argon, nitrogen and glycerin) were evaluated. Surface roughness (SR), surface elemental composition (energy-dispersive X-ray spectrometry, EDS) and biofilm formation (S. mutans and oral microcosm) were assessed. Surfaces were observed using scanning electron microscopy (SEM). Microbiological tests were performed with and without saliva pre-conditioning of the surfaces. EDS analysis was performed before and after biofilm formation, and biofilm morphology was evaluated using confocal laser scanning microscopy (CLSM). Data were analyzed using multi-way ANOVA and Tukey post-hoc test (p < 0.05). RESULTS: Interfacial curing conditions significantly influenced SR depending on the tested RBC. EDS analysis showed that surface elemental composition was significantly influenced by the interfacial curing condition depending on the tested RBC. Interfacial curing conditions significantly influenced biofilm formation in both microbiological models in the absence of saliva pre-conditioning, depending on the tested RBC, whereas saliva pre-conditioning abrogated these effects. CONCLUSIONS: Surface curing conditions significantly impacted biofilm formation in a material-dependent manner, which was abrogated when surfaces were pre-conditioned with saliva. CLINICAL SIGNIFICANCE: Curing under glycerin did not improve the microbiological performances of the tested RBCs. These results, needing to be confirmed by in vivo data, have the potential to simplify operative procedures in restorative dentistry.
Authors: Sanaa M F Gad El-Rab; Amal A Ashour; Sakeenabi Basha; Amal Ahmed Alyamani; Nayef H Felemban; Enas Tawfik Enan Journal: Molecules Date: 2021-11-12 Impact factor: 4.411
Authors: Adriano Casaglia; Maria Antonietta Cassini; Roberta Condò; Flavia Iaculli; Loredana Cerroni Journal: Int J Environ Res Public Health Date: 2021-05-28 Impact factor: 3.390