Manja Kurečič1,2, Tomaž Rijavec3, Silvo Hribernik1, Aleš Lapanje3, Karin S Kleinschek1,2, Uroš Maver4. 1. Laboratory for Characterization & Processing of Polymers, Faculty of Mechanical Engineering, University of Maribor, Smetanova 17, SI-2000 Maribor, Slovenia. 2. Institute for Chemistry & Technology of Materials, Graz University of Technology, Stremayrgasse 9, AT-8010 Graz, Austria. 3. Department for Environmental Sciences, Jožef Stefan Institute, Jamova 39, SI-1000 Ljubljana, Slovenia. 4. Department of Pharmacology, Faculty of Medicine, Institute of Biomedical Sciences & University of Maribor, Taborska ulica 8, SI-2000 Maribor, Slovenia.
Abstract
AIM: A novel electrospun biocompatible nanofibrous material loaded with commensal bacteria for potential preventive treatment of the diabetic foot was developed. MATERIALS & METHODS: Two biocompatible polymers (carboxymethylcellulose and polyethylene oxide) were combined with a bacterium isolate from the skin located between the toes of a healthy adult (identified using a matrix-assisted laser desorption/ionization mass spectrometry-based method as a strain of Staphylococcus epidermidis). Higher bacteria loads in the material were assured through their encapsulation in polyethylenimine. The nanofibrous material was characterized using scanning electron microscopy, zeta-potential measurements and through evaluation of cell growth and viability. RESULTS & DISCUSSION: nanometer formation was confirmed using scanning electron microscopy, while the zeta-potential measurements revealed successful bacteria encapsulation. Viable and sufficiently growing cells were confirmed prior and after their incorporation. CONCLUSION: The prepared materials were proven suitable to deliver viable commensal bacteria in a comparable share to the Staphylococcaceae in the foot microbiome making this approach promising for preventive diabetic foot treatment.
AIM: A novel electrospun biocompatible nanofibrous material loaded with commensal bacteria for potential preventive treatment of the diabetic foot was developed. MATERIALS & METHODS: Two biocompatible polymers (carboxymethylcellulose and polyethylene oxide) were combined with a bacterium isolate from the skin located between the toes of a healthy adult (identified using a matrix-assisted laser desorption/ionization mass spectrometry-based method as a strain of Staphylococcus epidermidis). Higher bacteria loads in the material were assured through their encapsulation in polyethylenimine. The nanofibrous material was characterized using scanning electron microscopy, zeta-potential measurements and through evaluation of cell growth and viability. RESULTS & DISCUSSION: nanometer formation was confirmed using scanning electron microscopy, while the zeta-potential measurements revealed successful bacteria encapsulation. Viable and sufficiently growing cells were confirmed prior and after their incorporation. CONCLUSION: The prepared materials were proven suitable to deliver viable commensal bacteria in a comparable share to the Staphylococcaceae in the foot microbiome making this approach promising for preventive diabetic foot treatment.
Authors: Panna Vass; Eszter Pantea; András Domokos; Edit Hirsch; Júlia Domján; Áron Németh; Mónika Molnár; Csaba Fehér; Sune K Andersen; Tamás Vigh; Geert Verreck; István Csontos; György Marosi; Zsombor K Nagy Journal: AAPS PharmSciTech Date: 2020-07-31 Impact factor: 3.246
Authors: Brixhilda Domi; Carlos Rumbo; Javier García-Tojal; Livia Elena Sima; Gabriela Negroiu; Juan Antonio Tamayo-Ramos Journal: Int J Mol Sci Date: 2019-12-27 Impact factor: 5.923