Ali Taghizadehghalehjoughi1, Ahmet Hacimuftuoglu2, Meltem Cetin3, Afife Busra Ugur3, Bianca Galateanu4, Yaroslav Mezhuev5, Ufuk Okkay2, Numan Taspinar2, Mehmet Taspinar6, Abdullah Uyanik7, Betul Gundogdu8, Maryam Mohammadzadeh2, Kemal Alp Nalci2, Polychronis Stivaktakis9,10, Aristidis Tsatsakis9,10, Tae Woo Jung11, Ji Hoon Jeong12, A M Abd El-Aty2,13. 1. Department of Pharmacology and Toxicology, Faculty of Veterinary Science, Atatürk University, 25240, Erzurum, Turkey. 2. Department of Medical Pharmacology, Faculty of Medicine, Atatürk University, 25240, Erzurum, Turkey. 3. Department of Pharmaceutical Technology, Faculty of Pharmacy, Atatürk University, 25240, Erzurum, Turkey. 4. Department of Biochemistry and Molecular Biology, 91-95 Splaiul Independentei, 050095, District 5, Bucharest, Romania. 5. Center of Biomaterials, D Mendeleev University of Chemical Technology of Russia, Miusskaya Sq. 9, 125047 Moscow, Russia. 6. Department of Medical Biology, School of Medicine, Yuzuncu Yil University, Van, Turkey. 7. Department of Nephrology, Faculty of Medicine, Ataturk University, 25240, Erzurum, Turkey. 8. Department of Pathology, Faculty of Medicine, Ataturk University, 25240, Erzurum, Turkey. 9. Laboratory of Toxicology, School of Medicine, University of Crete, 71003, Heraklion, Greece. 10. Toxplus SA, University of Crete Spin-Off, 71601, Heraklion, Greece. 11. Research Administration Team, Seoul National University Bundang Hospital, Gyeonggi, Republic of Korea. 12. Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul, Republic of Korea. 13. Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
Abstract
AIM: The present study was designed to evaluate the effects of irinotecan hydrochloride (IRI)- or metformin hydrochloride (MET)-loaded poly-lactic-co-glycolic acid (PLGA) nanoparticles (NPs) for the treatment of glioblastoma multiforme using in vitro neuron and U-87 MG glioblastoma cell cultures and in vivo animal model. METHODS: The cytotoxic and neurotoxic effects of pure drugs, blank NPs and MET- and IRI-loaded PLGA NPs were investigated in vitro (using methylthiazolyldiphenyl-tetrazolium bromide assay) and in vivo (using Cavalieri's principle for estimation of cancer volume). RESULTS: 1 and 2 mM doses of MET and MET-loaded PLGA NPs, respectively, significantly reduced the volume of extracted cancer. CONCLUSION: Consequently, MET- and IRI-loaded PLGA NPs may be a promising approach for the treatment of glioblastoma multiforme.
AIM: The present study was designed to evaluate the effects of irinotecan hydrochloride (IRI)- or metformin hydrochloride (MET)-loaded poly-lactic-co-glycolic acid (PLGA) nanoparticles (NPs) for the treatment of glioblastoma multiforme using in vitro neuron and U-87 MG glioblastoma cell cultures and in vivo animal model. METHODS: The cytotoxic and neurotoxic effects of pure drugs, blank NPs and MET- and IRI-loaded PLGA NPs were investigated in vitro (using methylthiazolyldiphenyl-tetrazolium bromide assay) and in vivo (using Cavalieri's principle for estimation of cancer volume). RESULTS: 1 and 2 mM doses of MET and MET-loaded PLGA NPs, respectively, significantly reduced the volume of extracted cancer. CONCLUSION: Consequently, MET- and IRI-loaded PLGA NPs may be a promising approach for the treatment of glioblastoma multiforme.
Authors: Norhan Elshony; Atef M K Nassar; Yasser S El-Sayed; Dalia Samak; Ahmed Noreldin; Lamiaa Wasef; Hamida Saleh; Yaser H A Elewa; Shereen E Tawfeek; Abdullah A Saati; Gaber El-Saber Batiha; Michał Tomczyk; Masakazu Umezawa; Hazem M Shaheen Journal: Front Neurosci Date: 2021-05-14 Impact factor: 4.677