Huimin Qian1, Guoye Liu2, Yin Chen2, Ping Ma2,3, Xiaoxiao Kong1, Lu Zhou1, Jie Hong1, Changjun Bao1, Bing Gu2,3. 1. Department of Acute Infectious Disease Prevention and Control, Jiangsu Provincial Center for Disease Prevention and Control, Nanjing 210029, China. 2. Medical Technology School, Xuzhou Medical University, Xuzhou 221004, China. 3. Department of Laboratory Medicine, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, China.
Abstract
BACKGROUND: The objective of this study is to evaluate the prevalence of Shigella sonnei (S. sonnei) and characterize the mechanism of its increasing resistance to cefotaxime, a third-generation cephalosporin agent between 2012 and 2015. METHODS: We investigated the drug resistance in 95 isolates of S. sonnei by K-B dilution method and isolates with the extended-spectrum beta-lactamases (ESBLs)-producing genes were detected by polymerase chain reaction (PCR) and sequencing. RESULTS: Over a 4-year period, the resistance rate of S. sonnei to cefotaxime increased from 31.6% to 64.3%, between 2012 and 2015. Molecular characterization of the ESBL genes, comprising 28 strains of CTX-M-1 group: blaCTX-M-55 (n=22), blaCTX-M-3 (n=3) and blaCTX-M-15 (n=3); 11 strains of CTX-M-9 group: blaCTX-M-14 (n=9) and blaCTX-M-65 (n=2), and 36 strains with blaTEM-1 gene. None of S. sonnei isolates carried blaCTX-M-2 group and SHV-type. CONCLUSIONS: The antimicrobial resistance rate of S. sonnei to cefotaxime significantly increased. Accordingly, regular surveillance of the cephalosporin-resistant S. sonnei should be emphasized. Moreover, exploration of the mechanism underlying the resistance of S. sonnei to cefotaxime contributes to the prophylaxis of further emergence of drug resistance.
BACKGROUND: The objective of this study is to evaluate the prevalence of Shigella sonnei (S. sonnei) and characterize the mechanism of its increasing resistance to cefotaxime, a third-generation cephalosporin agent between 2012 and 2015. METHODS: We investigated the drug resistance in 95 isolates of S. sonnei by K-B dilution method and isolates with the extended-spectrum beta-lactamases (ESBLs)-producing genes were detected by polymerase chain reaction (PCR) and sequencing. RESULTS: Over a 4-year period, the resistance rate of S. sonnei to cefotaxime increased from 31.6% to 64.3%, between 2012 and 2015. Molecular characterization of the ESBL genes, comprising 28 strains of CTX-M-1 group: blaCTX-M-55 (n=22), blaCTX-M-3 (n=3) and blaCTX-M-15 (n=3); 11 strains of CTX-M-9 group: blaCTX-M-14 (n=9) and blaCTX-M-65 (n=2), and 36 strains with blaTEM-1 gene. None of S. sonnei isolates carried blaCTX-M-2 group and SHV-type. CONCLUSIONS: The antimicrobial resistance rate of S. sonnei to cefotaxime significantly increased. Accordingly, regular surveillance of the cephalosporin-resistant S. sonnei should be emphasized. Moreover, exploration of the mechanism underlying the resistance of S. sonnei to cefotaxime contributes to the prophylaxis of further emergence of drug resistance.
Entities:
Keywords:
Shigella sonnei (S. sonnei); extended-spectrum beta-lactamases (ESBLs); resistance
Authors: S Qiu; X Xu; C Yang; J Wang; B Liang; P Li; H Li; S Yi; H Liu; X Cui; Z Wu; J Xie; L Jia; L Wang; R Hao; H Jin; Y Wang; Y Sun; H Song Journal: Clin Microbiol Infect Date: 2014-11-07 Impact factor: 8.067
Authors: K L Kotloff; J P Winickoff; B Ivanoff; J D Clemens; D L Swerdlow; P J Sansonetti; G K Adak; M M Levine Journal: Bull World Health Organ Date: 1999 Impact factor: 9.408
Authors: Edgar I Campos-Madueno; Odette J Bernasconi; Aline I Moser; Peter M Keller; Francesco Luzzaro; Carola Maffioli; Thomas Bodmer; Andreas Kronenberg; Andrea Endimiani Journal: Antimicrob Agents Chemother Date: 2020-09-21 Impact factor: 5.191