Juan Lv1, Lanxiu Cao2, Rui Zhang3, Fu Bai4, Pengfei Wei5. 1. MD, Physician, Department of Traditional Chinese Medicine, Shanxi University, China. Acquisition, analysis and interpretation of data; manuscript preparation. 2. MD, Physician, Department of Prescription, Basic Medical College, Shanxi University of Traditional Chinese Medicine, China. Conception and design of the study, manuscript preparation, final approval. 3. MD, Physician, Department of Diabetes, Second Affiliated Hospital, Shanxi University of Traditional Chinese Medicine, China. Technical procedures and acquisition of data. 4. MD, Physician, Department of Traditional Chinese Medicine, Shanxi University, China. Technical procedures. 5. MD, Physician, Department of Radiotherapy, First Affiliated Hospital, Shanxi University of Traditional Chinese Medicine, China. Technical procedures.
Abstract
PURPOSE: To investigate the specific molecular mechanisms and effects of curcumin derivative J147 on diabetic peripheral neuropathy (DPN). METHODS: We constructed streptozotocin (STZ)-induced DPN rat models to detected mechanical withdrawal threshold (MWT) in vivo using Von Frey filaments. In vitro, we measured cell viability and apoptosis, adenosine 5'-monophosphate-activated protein kinase (AMPK) and transient receptor potential A1 (TRPA1) expression using MTT, flow cytometry, qRT-PCR and western blot. Then, TRPA1 expression level and calcium reaction level were assessed in agonist AICAR treated RSC96cells. RESULTS: The results showed that J147reduced MWT in vivo, increased the mRNA and protein level of AMPK, reduced TRPA1 expression and calcium reaction level in AITCR treated RSC96 cells, and had no obvious effect on cell viability and apoptosis. Besides, AMPK negative regulated TRPA1 expression in RSC96 cells. CONCLUSIONS: J147 could ameliorate DPN via negative regulation AMPK on TRPA1 in vivo and in vitro. A curcumin derivative J147might be a new therapeutic potential for the treatment of DPN.
PURPOSE: To investigate the specific molecular mechanisms and effects of curcumin derivative J147 on diabetic peripheral neuropathy (DPN). METHODS: We constructed streptozotocin (STZ)-induced DPNrat models to detected mechanical withdrawal threshold (MWT) in vivo using Von Frey filaments. In vitro, we measured cell viability and apoptosis, adenosine 5'-monophosphate-activated protein kinase (AMPK) and transient receptor potential A1 (TRPA1) expression using MTT, flow cytometry, qRT-PCR and western blot. Then, TRPA1 expression level and calcium reaction level were assessed in agonist AICAR treated RSC96cells. RESULTS: The results showed that J147reduced MWT in vivo, increased the mRNA and protein level of AMPK, reduced TRPA1 expression and calcium reaction level in AITCR treated RSC96 cells, and had no obvious effect on cell viability and apoptosis. Besides, AMPK negative regulated TRPA1 expression in RSC96 cells. CONCLUSIONS: J147 could ameliorate DPN via negative regulation AMPK on TRPA1 in vivo and in vitro. A curcumin derivative J147might be a new therapeutic potential for the treatment of DPN.
Authors: Sarah O A M Costa; Ianny B Rodrigues; Alysson V Braga; Bárbara C M Barbosa; Roger R L Silva; Felipe F Rodrigues; Ivo S F Melo; Marcela Í Morais; Brenda F M Castro; Armando S Cunha Júnior; Vinícius G Maltarollo; Renata B Oliveira; Márcio M Coelho; Renes R Machado Journal: Inflammopharmacology Date: 2022-01-30 Impact factor: 4.473